Does progesterone inhibit bacteria-stimulated interleukin-8 production by lower genital tract epithelial cells?
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Morgan R. Peltier
Abstract
Objective: Progesterone (P4) has been clinically shown to prevent the recurrence of preterm birth. The mechanism(s) of action is unclear, but may involve modulation of the immunologic inflammatory response of the lower genital tract. We evaluated the effects of P4 on interleukin-8 (IL-8) production by vaginal and cervical epithelial cells stimulated with bacterial species that are commonly associated with preterm birth.
Methods: Vaginal and endocervical epithelial cells were incubated with up to 10,000 ng/mL P4 overnight and stimulated with heat-killed Escherichia coli,Gardnerella vaginalis, or Ureaplasma urealyticum. Concentrations of IL-8 in conditioned medium were quantified by ELISA and viability of the cell cultures was measured by the reduction of a tetrazolium salt.
Results:E. coli, G. vaginalis and U. urealyticum-stimulated IL-8 production for both cell lines. P4 inhibited basal and bacteria-stimulated IL-8 production for vaginal epithelial cells but enhanced IL-8 production by endocervical cells. P4 reduced the number of viable cells for both cell lines.
Conclusions: P4 inhibits IL-8 production by vaginal epithelial cells stimulated with pathogens associated with preterm birth, possibly by reducing the number of viable cells or by inhibiting their proliferation. Although P4 also reduces proliferation of endocervical cells it also increases their production of IL-8.
©2009 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Original articles – Obstetrics
- The effect of bilateral pelvic neurectomy on uterine and abdominal electrical and pressure activity, as measured by telemetry in conscious, unrestrained pregnant rats
- Parental mental representations during late pregnancy and early parenthood following assisted reproductive technology
- Does progesterone inhibit bacteria-stimulated interleukin-8 production by lower genital tract epithelial cells?
- Flowcytometric assessment of fetomaternal hemorrhage during external cephalic version at term
- Progesterone – induced blocking factor (PIBF) and Th1/Th2 cytokine in women with threatened spontaneous abortion
- Coagulation and fibrinolysis related cytokine imbalance in preeclampsia: the role of placental trophoblasts
- Maternal serum adiponectin multimers in preeclampsia
- Effect of maternal smoking on cord blood estriol, placental lactogen, chorionic gonadotropin, FSH, LH, and cortisol
- Changes in symphysis pubis width during labor
- Temporal trends in pregnancy weight gain and birth weight in Bavaria 2000–2007: slightly decreasing birth weight with increasing weight gain in pregnancy
- Original articles – Fetus
- Embryo vascularization by three-dimensional power Doppler ultrasonography at 7–10 weeks of pregnancy
- Determination of fetal heart rate reactivity from a single 20-min window of non-stress testing in compromised fetuses
- Detailed multigrade evaluation of fetal disorders with the quantified actocardiogram
- Original articles – Newborn
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- Oxygen saturation in healthy newborns; influence of birth weight and mode of delivery
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- Letters to the Editor
- Obstetrical chondro-epiphyseal separation of the distal humerus: a case report and review of literature
- Post-term pregnancy as a risk factor for intrapartum fetal distress
- Severe neonatal hyporegenerative anemia due to anti-Vw (anti-MNS9) alloantibody
- A new device to facilitate the chorion villus sampling
- Correct definition of pentalogy of Cantrell
- Congress Calender
- Congress Calendar
