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Flowcytometric assessment of fetomaternal hemorrhage during external cephalic version at term
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Christoph Scholz
Veröffentlicht/Copyright:
17. März 2009
Abstract
External cephalic version (ECV) at term is a safe procedure and reduces the incidence of cesarean sections for breech presentation. One of the known complications, however, is an ECV-related disruption of the placental barrier and a subsequent transfusion of fetal blood into maternal circulation. While the incidence of ECV-related fetomaternal hemorrhage (FMH) has been determined recently in a large trial using a manual Kleihauer-Betke test (KBT), questions remain on the amount of ECV-related FMH. KBT, which detects fetal red blood cells (RBC) on the basis of acidic resistance of fetal hemoglobin (HbF), is known to be a sensitive test, yet prone to procedural errors limiting its accuracy in quantifying FMH. In this study we investigated 50 patients for FMH before and after ECV, using a dual-color flow cytometric test kit with a lower limit of quantification of 0.05% fetal RBC in maternal peripheral blood. Three patients had a quantifiable increase of fetal RBC detected after ECV (0.06%; 0.08%; 0.1%). None of these subtle increments was predictable by ECV-related clinical parameters or translated into fetal compromise. Using a sensitive and accurate flow cytometric test method, our data provide further assurance to mothers on the safety of ECV at term.
Keywords: Carbonic anhydrase; external cephalic version; F-cells; fetomaternal hemorrhage; flow cytometry
Received: 2008-9-9
Revised: 2008-11-24
Accepted: 2008-11-27
Published Online: 2009-03-17
Published Online: 2009-03-17
Published in Print: 2009-07-01
©2009 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
Carbonic anhydrase;
external cephalic version;
F-cells;
fetomaternal hemorrhage;
flow cytometry
Artikel in diesem Heft
- Original articles – Obstetrics
- The effect of bilateral pelvic neurectomy on uterine and abdominal electrical and pressure activity, as measured by telemetry in conscious, unrestrained pregnant rats
- Parental mental representations during late pregnancy and early parenthood following assisted reproductive technology
- Does progesterone inhibit bacteria-stimulated interleukin-8 production by lower genital tract epithelial cells?
- Flowcytometric assessment of fetomaternal hemorrhage during external cephalic version at term
- Progesterone – induced blocking factor (PIBF) and Th1/Th2 cytokine in women with threatened spontaneous abortion
- Coagulation and fibrinolysis related cytokine imbalance in preeclampsia: the role of placental trophoblasts
- Maternal serum adiponectin multimers in preeclampsia
- Effect of maternal smoking on cord blood estriol, placental lactogen, chorionic gonadotropin, FSH, LH, and cortisol
- Changes in symphysis pubis width during labor
- Temporal trends in pregnancy weight gain and birth weight in Bavaria 2000–2007: slightly decreasing birth weight with increasing weight gain in pregnancy
- Original articles – Fetus
- Embryo vascularization by three-dimensional power Doppler ultrasonography at 7–10 weeks of pregnancy
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- Original articles – Newborn
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