Accuracy and influence of ascorbic acid on glucose-test with urine dip sticks in prenatal care
-
Claudia Ritterath
Abstract
Objective: In Germany, urine analysis with dip sticks are used for screening of gestational diabetes. Our goal was to find the cut-off levels of glucose dip sticks and evaluate the influence of ascorbic acid on the result.
Study design: We took urine samples of 152 pregnant patients between 01/2004 and 10/2004. The glucose content of the urine was analyzed using several dip sticks (Combur10® Roche®, Multistix® 8SG Bayer®, Diabur5000® Roche®) and by the hexokinase method. The ascorbic acid concentration of the specimen was determined.
Results: Glycosuria test dip sticks have a good sensitivity and specificity of the expected positive/negative-reaction compared with the hexokinase method but showed a significant reduction of the color reaction at high concentrations of ascorbic acid.
Conclusion: High ascorbic acid concentrations cause a reduction in the color reaction of urine dip sticks. False-negative test stick measurements can appear in patients with low glycosuria. The rate of glycosuria shows high individual differences and dependent on the concentration of vitamin C. We conclude that dip sticks are not useful in prenatal management.
References
1 American Diabetes Association: Gestational Diabetes Mellitus. Diabetes Care 25 (Suppl 1) (2002) S9410.2337/diacare.25.2007.S94Suche in Google Scholar
2 Bayer® Vital Product Information: Bayer®, Munich, Germany 2002Suche in Google Scholar
3 Brigden ML, D Edgell, M McPherson, A Leadbeater, G Hoang: High incidence of the significant urinary ascorbic acid concentrations in a West Coast population – implications for routine urinanalysis. Clin Chem38 (1991) 426Suche in Google Scholar
4 Buhling Kai J, L Elze, H Henrich, et al.: The usefulness of glycouria and the influence of maternal blood pressure in screening for gestation diabetes. Eur J Obstet Gyn Repro Biol113 (2004) 145Suche in Google Scholar
5 Conrad KP. Mechanism use of renal vasodilatation and hyperfiltration during pregnancy. J Soc Gynecol Investig11 (2004) 43810.1016/j.jsgi.2004.05.002Suche in Google Scholar PubMed
6 Deutsche Diabetes Gesellschaft: Diagnostik und Therapie des Gestations diabetes: http://www.deutsche-diabetes-gesellschaft.de, 2001Suche in Google Scholar
7 Hooper DE: Detecting GD and preeclampsia. Effectiveness of routine urine screening for glucose and protein. J Reprod Med41 (1996) 885Suche in Google Scholar
8 Roche® Product Information: Roche Diagnostics®, Mannheim, Germany 2001Suche in Google Scholar
9 Sutherland HW, JM Stowers, RJ Christie: Factors affecting sensitivity of glucose-oxidase strips used to test for glycosuria. Lancet1 (1970) 1071Suche in Google Scholar
10 Yamane N, F Sakamoto, F Matsuura, et al.: Quantification of urinary glucose and protein with test strips through reflectometric analysis. Clin Biochem21 (1988) 271Suche in Google Scholar
©2006 by Walter de Gruyter Berlin New York
Artikel in diesem Heft
- Advances in Perinatal Medicine - 5th Annual meeting of the Italian Society of Perinatal Medicine held in Parma, Italy on June 15th – 17th 2006
- Hemorrhagic shock in obstetrics
- Altered protease expression by periarterial trophoblast cells in severe early-onset preeclampsia with IUGR
- Gestational diabetes mellitus in patients receiving long-term corticosteroid therapy during pregnancy
- Accuracy and influence of ascorbic acid on glucose-test with urine dip sticks in prenatal care
- Protein C, protein S, and thrombomodulin in amniotic fluid. A preliminary study
- Timing of cord clamping revisited
- Intrapartum cardiotocography – the dilemma of interpretational variation
- Maturation of the autonomic nervous system: differences in heart rate variability in premature vs. term infants
- Four chamber view plus three-vessel and trachea view for a complete evaluation of the fetal heart during the second trimester
- Pleural fluid/serum immunoglobulin ratio is a diagnostic marker for congenital chylothorax in utero
- Fetal brain sparing is strongly related to the degree of increased placental vascular impedance
- Outcome of fetuses in women with pregestational diabetes mellitus
- Outcome of fetuses in women with pregestational diabetes mellitus
- Inhaled nitric oxide therapy might reduce the need for hyperventilation therapy in infants with persistent pulmonary hypertension of the newborn
- To drain or not to drain: a single institution experience with neonatal intestinal perforation
- Infective puerperal endocarditis caused by Escherichia coli
- Correlation between plasma and urinary caffeine levels in preterm infants
- Fetal ascites secondary to urinary hydrocolpos
- Congress Calendar
- Suppression of IL-2 and IFN-γ production in women with spontaneous preterm labor
Artikel in diesem Heft
- Advances in Perinatal Medicine - 5th Annual meeting of the Italian Society of Perinatal Medicine held in Parma, Italy on June 15th – 17th 2006
- Hemorrhagic shock in obstetrics
- Altered protease expression by periarterial trophoblast cells in severe early-onset preeclampsia with IUGR
- Gestational diabetes mellitus in patients receiving long-term corticosteroid therapy during pregnancy
- Accuracy and influence of ascorbic acid on glucose-test with urine dip sticks in prenatal care
- Protein C, protein S, and thrombomodulin in amniotic fluid. A preliminary study
- Timing of cord clamping revisited
- Intrapartum cardiotocography – the dilemma of interpretational variation
- Maturation of the autonomic nervous system: differences in heart rate variability in premature vs. term infants
- Four chamber view plus three-vessel and trachea view for a complete evaluation of the fetal heart during the second trimester
- Pleural fluid/serum immunoglobulin ratio is a diagnostic marker for congenital chylothorax in utero
- Fetal brain sparing is strongly related to the degree of increased placental vascular impedance
- Outcome of fetuses in women with pregestational diabetes mellitus
- Outcome of fetuses in women with pregestational diabetes mellitus
- Inhaled nitric oxide therapy might reduce the need for hyperventilation therapy in infants with persistent pulmonary hypertension of the newborn
- To drain or not to drain: a single institution experience with neonatal intestinal perforation
- Infective puerperal endocarditis caused by Escherichia coli
- Correlation between plasma and urinary caffeine levels in preterm infants
- Fetal ascites secondary to urinary hydrocolpos
- Congress Calendar
- Suppression of IL-2 and IFN-γ production in women with spontaneous preterm labor
