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Identification and functional characterization of a sesquiterpene synthase gene from Eleutherococcus trifoliatus
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Chi-Hsiang Wen
Veröffentlicht/Copyright:
11. August 2011
Abstract
In the present study, one sesquiterpene synthase gene in Eleutherococcustrifoliatus was identified and characterized. Full-length cDNA was obtained from stems. It contained an open reading frame of 1671 bp (EtCop) with a predicted molecular mass of 64.5 kDa. The amino acid sequence of EtCop contained the common terpene synthase family motifs RR(x)8W, RxR and DDxxD. The recombinant protein from Escherichia coli was incubated with farnesyl diphosphate in order to identify the function of EtCop. The product of EtCop could be identified as an α-copaene by means of gas chromatography-mass spectrometry analysis and comparison with an authentic standard.
Received: 2011-4-11
Accepted: 2011-7-7
Published Online: 2011-08-11
Published in Print: 2012-02-01
©2012 by Walter de Gruyter Berlin Boston
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Artikel in diesem Heft
- Original Papers
- Oxidative stabilisation of kraft lignin for carbon fibre production
- Antithrombotic properties of sulfated wood-derived galactoglucomannans
- Recycling potential of unbleached and bleached chemical pulps from juvenile and mature wood of Populus deltoides
- Natural and artificial ageing of spruce wood as observed by FTIR-ATR and UVRR spectroscopy
- Characterization of phenolic compounds from inner bark of Betula pendula
- Identification and functional characterization of a sesquiterpene synthase gene from Eleutherococcus trifoliatus
- Microstructure and stiffness of Scots pine (Pinus sylvestris L) sapwood degraded by Gloeophyllum trabeum and Trametes versicolor – Part I: Changes in chemical composition, density and equilibrium moisture content
- Changes in microstructure and stiffness of Scots pine (Pinus sylvestris L) sapwood degraded by Gloeophyllum trabeum and Trametes versicolor – Part II: Anisotropic stiffness properties
- Off-axis Young’s modulus and off-axis shear modulus of wood measured by flexural vibration tests
- Empirical models for radial and tangential fibre width in tree rings of Norway spruce in north-western Europe
- Automatic grain texture analysis using integral transforms
- The fungal resistance of wood modified with glutaraldehyde
- Relative fungal efficacy results from the soil block test with a long incubation period of three commercial copper wood preservatives
- Uneven distribution of preservative in kiln-dried sapwood lumber of Scots pine: Impact of wood structure and resin allocation
- Utilization of surfactants for consolidation of wooden work of art
- Screening for xylanase and β-xylosidase production from wood-inhabiting Penicillium strains for potential use in biotechnological applications
- Short Note
- Furfuryl alcohol impregnation for improved plasticization and fixation during the densification of wood
- Meetings
- Meetings
Artikel in diesem Heft
- Original Papers
- Oxidative stabilisation of kraft lignin for carbon fibre production
- Antithrombotic properties of sulfated wood-derived galactoglucomannans
- Recycling potential of unbleached and bleached chemical pulps from juvenile and mature wood of Populus deltoides
- Natural and artificial ageing of spruce wood as observed by FTIR-ATR and UVRR spectroscopy
- Characterization of phenolic compounds from inner bark of Betula pendula
- Identification and functional characterization of a sesquiterpene synthase gene from Eleutherococcus trifoliatus
- Microstructure and stiffness of Scots pine (Pinus sylvestris L) sapwood degraded by Gloeophyllum trabeum and Trametes versicolor – Part I: Changes in chemical composition, density and equilibrium moisture content
- Changes in microstructure and stiffness of Scots pine (Pinus sylvestris L) sapwood degraded by Gloeophyllum trabeum and Trametes versicolor – Part II: Anisotropic stiffness properties
- Off-axis Young’s modulus and off-axis shear modulus of wood measured by flexural vibration tests
- Empirical models for radial and tangential fibre width in tree rings of Norway spruce in north-western Europe
- Automatic grain texture analysis using integral transforms
- The fungal resistance of wood modified with glutaraldehyde
- Relative fungal efficacy results from the soil block test with a long incubation period of three commercial copper wood preservatives
- Uneven distribution of preservative in kiln-dried sapwood lumber of Scots pine: Impact of wood structure and resin allocation
- Utilization of surfactants for consolidation of wooden work of art
- Screening for xylanase and β-xylosidase production from wood-inhabiting Penicillium strains for potential use in biotechnological applications
- Short Note
- Furfuryl alcohol impregnation for improved plasticization and fixation during the densification of wood
- Meetings
- Meetings
