Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi
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Ulrika Råberg
Abstract
In the present work PCR technology was used as a tool to detect the early stages of wood decay and was compared with microscopic evaluation. The wood decay fungi Postia placenta and Coniophora puteana were detectable in interior wood samples by terminal restriction fragment length polymorphism (T-RFLP) after 2weeks of incubation with monocultures, while microscopic detection of hyphae was not possible until after 7 weeks. A potential problem when fungal communities are studied with T-RFLPs of rDNA is that intra-specific variation complicates data analysis. In this work, we show that intra-specific sequence variation in the internal transcribed spacer of the rDNA in Coniophora puteana allows T-RFLP identification of this species. This is due to intra-specific variations in fragment length, in combination with the absence of point mutations in the selected restriction sites.
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©2005 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Contents
- Species index (scientific names)
- Subject Index
- Acknowledgement
- Author Index
- Ultrastructural changes in a holocellulose pulp revealed by enzymes, thermoporosimetry and atomic force microscopy
- Development of wet strength additives from wheat gluten
- Characterization of electrolyzed magnesium spent-sulfite liquor
- Molecular weight-functional group relations in softwood residual kraft lignins
- Structure-activity relationships of cadinane-type sesquiterpene derivatives against wood-decay fungi
- Effect of water on wood liquefaction and the properties of phenolated wood
- Effect of wood species and molecular weight of phenolic resins on curing behavior and bonding development
- Contact-free measurement and non-linear finite element analyses of strain distribution along wood adhesive bonds
- Comparison between HT-dried and LT-dried spruce timber in terms of shape and dimensional stability
- Physical properties of earlywood and latewood of Pinus radiata D. Don: Anisotropic shrinkage, equilibrium moisture content and fibre saturation point
- Effect of stress levels on compressive low-cycle fatigue behaviour of softwood
- Comparison of morphological and chemical properties between juvenile wood and compression wood of loblolly pine
- Ultrastructure of commercial recycled pulp fibers for the production of packaging paper
- Oxalate regulation by two brown rot fungi decaying oxalate-amended and non-amended wood
- Pine and spruce roundwood species classification using multivariate image analysis on bark
- Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi
- Personalia
- Award presentation on the occasion of the 13th International Symposium on Wood, Fibre and Pulping Chemistry, May 16–19, 2005, Auckland, New Zealand
- NMR studies on Fraser fir Abies fraseri (Pursh) Poir. Lignins
Articles in the same Issue
- Contents
- Species index (scientific names)
- Subject Index
- Acknowledgement
- Author Index
- Ultrastructural changes in a holocellulose pulp revealed by enzymes, thermoporosimetry and atomic force microscopy
- Development of wet strength additives from wheat gluten
- Characterization of electrolyzed magnesium spent-sulfite liquor
- Molecular weight-functional group relations in softwood residual kraft lignins
- Structure-activity relationships of cadinane-type sesquiterpene derivatives against wood-decay fungi
- Effect of water on wood liquefaction and the properties of phenolated wood
- Effect of wood species and molecular weight of phenolic resins on curing behavior and bonding development
- Contact-free measurement and non-linear finite element analyses of strain distribution along wood adhesive bonds
- Comparison between HT-dried and LT-dried spruce timber in terms of shape and dimensional stability
- Physical properties of earlywood and latewood of Pinus radiata D. Don: Anisotropic shrinkage, equilibrium moisture content and fibre saturation point
- Effect of stress levels on compressive low-cycle fatigue behaviour of softwood
- Comparison of morphological and chemical properties between juvenile wood and compression wood of loblolly pine
- Ultrastructure of commercial recycled pulp fibers for the production of packaging paper
- Oxalate regulation by two brown rot fungi decaying oxalate-amended and non-amended wood
- Pine and spruce roundwood species classification using multivariate image analysis on bark
- Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi
- Personalia
- Award presentation on the occasion of the 13th International Symposium on Wood, Fibre and Pulping Chemistry, May 16–19, 2005, Auckland, New Zealand
- NMR studies on Fraser fir Abies fraseri (Pursh) Poir. Lignins