Production of 2,5-dimethoxyhydroquinone by the brown-rot fungus Serpula lacrymans to drive extracellular Fenton reaction
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T. Shimokawa
Abstract
The brown-rot fungus Serpula lacrymans MAFF 420003 was grown in a liquid culture medium containing 0.5% carboxymethyl cellulose (CMC) and 1% glucose as carbon sources. Although little extracellular cellulase was secreted, the fungus produced an oxidized quinone-type chelator, 2,5-dimethoxy-1,4-benzoquinone (2,5-DMBQ). The concentration of 2,5-DMBQ in the medium reached a maximum of 90 μmm after a month of cultivation. S. lacrymans could reduce 2,5-DMBQ to 2,5-dimethoxyhydroquinone (2,5-DMHQ), thus a biological Fenton reaction was adopted by the fungus. The changes in the molecular weight distribution of CMC and arabinogalactan were analyzed after the addition of 2,5-DMHQ and Fe3+. CMC was apparently depolymerized by the reaction, but the same reaction conditions showed no significant effect on arabinogalactan. These differences suggest the specificities of the biological Fenton reaction via 2,5-DMBQ toward soluble polysaccharides. In addition, the crystallinity index of α-cellulose did not decrease as a result of the reaction with 2,5-DMHQ and Fe3+. These results provide indirect evidence that S. lacrymans employs a biological Fenton reaction mediated by a quinone-type chelator, and preferentially degrades amorphous regions of cellulose rather than crystalline regions in the non-enzymatic cellulose degradation.
Copyright © 2004 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- The Bremen Cog of 1380 – An electron microscopic study of its degraded wood before and after stabilization with PEG
- Ultrastructure of iodine treated wood
- Effects of refining on the fibre structure of kraft pulps as revealed by FE-SEM and TEM: Influence of alkaline degradation
- Changes in the fiber wall during refining of bleached pine kraft pulp
- An unusual formation of tension wood in a natural forest Acacia sp.
- Changes in the surface properties of wood due to sanding
- Use of near infrared spectroscopy to predict the mechanical properties of six softwoods
- The creep of wood destabilized by change in moisture content. Part 1: The creep behaviors of wood during and immediately after drying
- Modeling the process of desorption of water in oak (Quercus robur L.) wood
- Moisture adsorption thermodynamics of wood from fractal-geometry approach
- Application of ionic liquids for electrostatic control in wood
- Ionic liquids in wood preservation
- New bis-quaternary ammonium and bis-imidazolium chloride wood preservatives
- Environmentally-benign wood preservatives based on an organic biocide:antioxidant combination: Ground-contact efficacy ratings and BHT depletion after four years of exposure
- Production of 2,5-dimethoxyhydroquinone by the brown-rot fungus Serpula lacrymans to drive extracellular Fenton reaction
- Fungal decay resistance of wood reacted with phosphorus pentoxide-amine system
- The use of organo alkoxysilane coupling agents for wood preservation
- Phenolic extractives from wood of birch (Betula pendula)
- Stability of arylglycerols during alkaline cooking
