Analysis of Protein Expression along the Normal to Tension Wood Gradient in Eucalyptus gunnii
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C. Plomion
Abstract
This paper examines the contribution of various xylem proteins to wood formation in Eucalyptus gunnii. Proteins were extracted from differentiating xylem harvested from a crooked tree, separated by high-resolution two-dimensional polyacrylamide gel electrophoresis, visualised by silver nitrate staining and analysed with a computer-assisted system for single protein spot quantification. Growth strain measurements allowed xylem samples to be classified quantitatively from tension wood to normal wood. Regression of lignin content on growth strain showed that a decrease in lignin content corresponded to decreasing growth strain values, i.e., presence of tension wood. Out of the 140 studied protein spots, 12 were significantly associated with growth strain: 7 being less abundant in tension wood and 5 being more abundant in tension wood. A clustered-correlation analysis was performed to study protein expression simultaneously along the gradient of gravistimulated stressed xylem tissue. Proteins were found to form “expression clusters”.
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Topochemical Characterisation of Phenolic Extractives in Discoloured Beechwood (Fagus sylvatica L.)
- Detection and Species Identification of Wood-Decaying Fungi by Hybridization of Immobilized Sequence-Specific Oligonucleotide Probes with PCR-Amplified Fungal Ribosomal DNA Internal Transcribed Spacers
- Analysis of Protein Expression along the Normal to Tension Wood Gradient in Eucalyptus gunnii
- Phenolic and Lipophilic Extractives in Scots Pine Knots and Stemwood
- Chemical Characterisation of Wood of Roman Ships Brought to Light in the Recently Discovered Ancient Harbour of Pisa (Tuscany, Italy)
- Lignin Structures in Normal and Compression Wood. Evaluation by Thioacidolysis Using Ethanethiol and Methanethiol
- Lignin-Carbohydrate Condensation Product Formation in a Biomimetic Model Pulp Bleaching System
- Viscoelastic Behaviour of Wood Fibres during the Hot Pressing of Medium Density Fibreboard
- Measuring Relative Fibre Length in Scots Pine by Non-Destructive Wood Sampling
- Modelling Log-End Cracks Due to Growth Stresses: Calculation of the Elastic Energy Release Rate
- Modeling Ovendry Softwood Resistivity Based on the Resistor Network Theory
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- A Theoretical Analysis of Timber Drying in Oscillating Climates
- Comparing the Effect of Chemical and Physical Properties on Complex Electrical Impedance of Scots Pine Wood
- Wood-Derived Porous Ceramics via Infiltration of SiO2-Sol and Carbothermal Reduction
- Manufacture of Fiberboard from Wood Fibers Activated with Fentons Reagent (H2O2/FeSO4)
