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Detection and Species Identification of Wood-Decaying Fungi by Hybridization of Immobilized Sequence-Specific Oligonucleotide Probes with PCR-Amplified Fungal Ribosomal DNA Internal Transcribed Spacers
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S. Oh
Published/Copyright:
June 1, 2005
Summary
We developed an effective detection method for wood-decaying fungi by hybridization of immobilized Sequence-Specific Oligonucleotide Probes with florescent-labeled PCR-amplified fungal rDNA internal transcribed spacer sequences. This method takes advantage of both the sequence specificity of Southern blot hybridization and the sensitivity of the previously reported PCR-based fungal species identification methods. Both in vitro cultured fungal strains and naturally decaying wood samples were used to demonstrate that this method is robust and practical for detection of incipient wood-decaying fungi. It can be a useful tool for microbial ecology, plant pathology, protection of wood products in service, preservation efforts for high-value furniture and wood-based art and DNA fingerprinting for tracking the source of contamination of wood decay fungi.
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Published Online: 2005-06-01
Published in Print: 2003-06-26
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Topochemical Characterisation of Phenolic Extractives in Discoloured Beechwood (Fagus sylvatica L.)
- Detection and Species Identification of Wood-Decaying Fungi by Hybridization of Immobilized Sequence-Specific Oligonucleotide Probes with PCR-Amplified Fungal Ribosomal DNA Internal Transcribed Spacers
- Analysis of Protein Expression along the Normal to Tension Wood Gradient in Eucalyptus gunnii
- Phenolic and Lipophilic Extractives in Scots Pine Knots and Stemwood
- Chemical Characterisation of Wood of Roman Ships Brought to Light in the Recently Discovered Ancient Harbour of Pisa (Tuscany, Italy)
- Lignin Structures in Normal and Compression Wood. Evaluation by Thioacidolysis Using Ethanethiol and Methanethiol
- Lignin-Carbohydrate Condensation Product Formation in a Biomimetic Model Pulp Bleaching System
- Viscoelastic Behaviour of Wood Fibres during the Hot Pressing of Medium Density Fibreboard
- Measuring Relative Fibre Length in Scots Pine by Non-Destructive Wood Sampling
- Modelling Log-End Cracks Due to Growth Stresses: Calculation of the Elastic Energy Release Rate
- Modeling Ovendry Softwood Resistivity Based on the Resistor Network Theory
- Comparison of the TL-Shear Strength of Normal and Compression Wood of European Larch
- A Theoretical Analysis of Timber Drying in Oscillating Climates
- Comparing the Effect of Chemical and Physical Properties on Complex Electrical Impedance of Scots Pine Wood
- Wood-Derived Porous Ceramics via Infiltration of SiO2-Sol and Carbothermal Reduction
- Manufacture of Fiberboard from Wood Fibers Activated with Fentons Reagent (H2O2/FeSO4)
