Ultrastructural Localisation of Glucomannan in Kraft Pulp Fibres
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I. Duchesne
Summary
The localisation of glucomannan on the surface of Norway spruce kraft pulp fibres with varying glucomannan (GM) contents (10.3 and 3.8%) was studied using immunogold labelling, rapid freeze deep etching (RFDE) followed by metal replication and transmission electron microscopy (TEM). The ultrastructure of P, S1 and S2 of pulp fibres with low hemicellulose content tended to be more compact than that of pulp fibres with high hemicellulose content, suggesting aggregation of cellulose microfibrils upon hemicellulose removal. The degree of immunogold labelling visualised as globular structures was similar for the two pulps, despite the large difference in total glucomannan content. Thus, no correlation between the bulk and surface contents of glucomannan was found. The globular structures were heterogeneously located along the cellulose macrofibrils (fibril aggregates) and their frequency varied greatly within and between TEM micrographs.
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- The Relationship Between Variability of Cell Wall Mass of Earlywood and Latewood Tracheids in Larch Tree-Rings, the Rate of Tree-Ring Growth and Climatic Changes
- How Variability in OSB Mechanical Properties Affects Biological Durability Testing
- Microfibril Angles Inside and Outside Crossfields of Norway Spruce Tracheids
- Reactivity of a Fungal Laccase Towards Lignin in Softwood Kraft Pulp
- Lignans and Lipophilic Extractives in Norway Spruce Knots and Stemwood
- Investigation of Lignin Oligomers Using Electrospray Ionisation Mass Spectrometry
- Reactions of Lignin with Peroxymonophosphoric Acid: The Degradation of Lignin Model Compounds
- The Reactions of Lignin Model Compounds with Hydrogen Peroxide at Low pH
- Spin Distribution in Dehydrogenated Coniferyl Alcohol and Associated Dilignol Radicals
- Ultrastructural Localisation of Glucomannan in Kraft Pulp Fibres
- New Method for Quantitative Preparation of Lignin- Carbohydrate Complex from Unbleached Softwood Kraft Pulp: Lignin-Polysaccharide Networks I
- Peroxide Bleaching of Parquet Blocks and Glue Lams
- Easily Degradable Chlorinated Compounds Derived from Glucuronoxylan in Filtrates from Chlorine Dioxide Bleaching of Eucalyptus globulus Kraft Pulp
- Non-Deterministic Description of Wood Radio Frequency Vacuum Drying
- Bending Creep of High-Temperature Dried Spruce Timber
- Optimization of a Violin Top with a Combined Laminate Theory and Honeycomb Model of Wood
- Optimisation of Soda Pulping Variables for Preparation of Dissolving Pulps from Oil Palm Fibre
Articles in the same Issue
- The Relationship Between Variability of Cell Wall Mass of Earlywood and Latewood Tracheids in Larch Tree-Rings, the Rate of Tree-Ring Growth and Climatic Changes
- How Variability in OSB Mechanical Properties Affects Biological Durability Testing
- Microfibril Angles Inside and Outside Crossfields of Norway Spruce Tracheids
- Reactivity of a Fungal Laccase Towards Lignin in Softwood Kraft Pulp
- Lignans and Lipophilic Extractives in Norway Spruce Knots and Stemwood
- Investigation of Lignin Oligomers Using Electrospray Ionisation Mass Spectrometry
- Reactions of Lignin with Peroxymonophosphoric Acid: The Degradation of Lignin Model Compounds
- The Reactions of Lignin Model Compounds with Hydrogen Peroxide at Low pH
- Spin Distribution in Dehydrogenated Coniferyl Alcohol and Associated Dilignol Radicals
- Ultrastructural Localisation of Glucomannan in Kraft Pulp Fibres
- New Method for Quantitative Preparation of Lignin- Carbohydrate Complex from Unbleached Softwood Kraft Pulp: Lignin-Polysaccharide Networks I
- Peroxide Bleaching of Parquet Blocks and Glue Lams
- Easily Degradable Chlorinated Compounds Derived from Glucuronoxylan in Filtrates from Chlorine Dioxide Bleaching of Eucalyptus globulus Kraft Pulp
- Non-Deterministic Description of Wood Radio Frequency Vacuum Drying
- Bending Creep of High-Temperature Dried Spruce Timber
- Optimization of a Violin Top with a Combined Laminate Theory and Honeycomb Model of Wood
- Optimisation of Soda Pulping Variables for Preparation of Dissolving Pulps from Oil Palm Fibre
