Identification of Indoor Rot Fungi by Taxon-Specific Priming Polymerase Chain Reaction
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Ute Moreth
Summary
The internal transcribed spacer (ITS) of the nuclear ribosomal DNA (rDNA) of the main fungal species causing wood rot damages in European buildings was amplified by the polymerase chain reaction (PCR). After sequencing the ITS, fungus-specific oligonucleotide primers were designed for taxon-specific priming PCR. These DNA marker molecules were suitable for the differential diagnosis of the Dry rot fungus, Serpula lacrymans, the Wild merulius, S. himantioides, the Oak polypore, Donkioporia expansa, the Brown cellar fungus, Coniophora puteana, the Broad-spored white polypore, Antrodia vaillantii, the Sap polypore, Tyromyces placenta, and the Yellow-red gill polypore, Gloeophyllum sepiarium. Each specific marker identified isolates of its respective target species. Cross reaction with ‘foreign’ fungi was the exception. Species detection from rot samples in buildings was possible, since DNA from contaminating organisms does not response to the marker molecules. The diagnosis was rapid, since preceding fungal pure cultures, special DNA extraction/purification and restriction by endonucleases are not required.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
Artikel in diesem Heft
- Identification of Indoor Rot Fungi by Taxon-Specific Priming Polymerase Chain Reaction
- Characteristics of Spruce [Picea abies (L.) Karst] Latewood Formed under Abnormally Low Temperatures
- The Chemical Nature of Kiln Brown Stain in Radiata Pine
- Microdistribution of Some Copper and Zinc Containing Waterborne and Organic Solvent Wood Preservatives in Spruce Wood Cell Walls
- X-Ray Diffraction as an Analytical Method in Wood Preservatives
- Modifications of Hybrid Walnut (Juglans nigra 23 x Juglans regia) Wood Colour and Phenolic Composition Under Various Steaming Conditions
- Incorporation of 13C-Labeled Coniferyl Alcohol into Developing Ginkgo biloba L. Lignin Revealed by Analytical Pyrolysis and CuO Oxidation in Combination with Isotope Ratio Monitoring-Gas Chromatography-Mass Spectrometry
- Atomic Force Microscopy (AFM) Investigation of Langmuir-Blodgett (LB) Films of Sugar Cane Bagasse Lignin
- Comparison of Acetic Acid Lignin with Milled Wood and Alkaline Lignins from Wheat Straw
- Monitoring of Lignin and Hemicelluloses in Spent Cooking Liquor during Kraft Delignification
- Preparation of Wood with Thermoplastic Properties, Part 1. Classical Synthesis
- Preparation of Wood with Thermoplastic Properties, Part 2. Simplified Technologies
- The Effect of Hydrostatic Pressure on Physical Properties and Microstructure of Spruce and Cherry
- Application of Liquefied Wood to a New Resin System—Synthesis and Properties of Liquefied Wood/Epoxy Resins
- Lab-Scale Synthesis of Isotopically Labeled Formaldehyde for the Production of Formaldehyde-Based Wood Adhesives
- Confirmation of the Presence of Formaldehyde and N-(Methylene)morpholinium Cations as Reactive Species in the Cellulose/NMMO/Water System by Trapping Reactions
- Compressive Creep of Wood under High Temperature Steam
- Literature Reports
Artikel in diesem Heft
- Identification of Indoor Rot Fungi by Taxon-Specific Priming Polymerase Chain Reaction
- Characteristics of Spruce [Picea abies (L.) Karst] Latewood Formed under Abnormally Low Temperatures
- The Chemical Nature of Kiln Brown Stain in Radiata Pine
- Microdistribution of Some Copper and Zinc Containing Waterborne and Organic Solvent Wood Preservatives in Spruce Wood Cell Walls
- X-Ray Diffraction as an Analytical Method in Wood Preservatives
- Modifications of Hybrid Walnut (Juglans nigra 23 x Juglans regia) Wood Colour and Phenolic Composition Under Various Steaming Conditions
- Incorporation of 13C-Labeled Coniferyl Alcohol into Developing Ginkgo biloba L. Lignin Revealed by Analytical Pyrolysis and CuO Oxidation in Combination with Isotope Ratio Monitoring-Gas Chromatography-Mass Spectrometry
- Atomic Force Microscopy (AFM) Investigation of Langmuir-Blodgett (LB) Films of Sugar Cane Bagasse Lignin
- Comparison of Acetic Acid Lignin with Milled Wood and Alkaline Lignins from Wheat Straw
- Monitoring of Lignin and Hemicelluloses in Spent Cooking Liquor during Kraft Delignification
- Preparation of Wood with Thermoplastic Properties, Part 1. Classical Synthesis
- Preparation of Wood with Thermoplastic Properties, Part 2. Simplified Technologies
- The Effect of Hydrostatic Pressure on Physical Properties and Microstructure of Spruce and Cherry
- Application of Liquefied Wood to a New Resin System—Synthesis and Properties of Liquefied Wood/Epoxy Resins
- Lab-Scale Synthesis of Isotopically Labeled Formaldehyde for the Production of Formaldehyde-Based Wood Adhesives
- Confirmation of the Presence of Formaldehyde and N-(Methylene)morpholinium Cations as Reactive Species in the Cellulose/NMMO/Water System by Trapping Reactions
- Compressive Creep of Wood under High Temperature Steam
- Literature Reports
