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Anti-neutrophil cytoplasmic antibodies (ANCA) specific for one or several antigens: useful markers for subtypes of ulcerative colitis and associated primary sclerosing cholangitis

  • Senka Dobric , Dragan Popovic , Milos Nikolic , Sladjana Andrejevic , Milan Spuran and Branka Bonaci-Nikolic EMAIL logo
Published/Copyright: November 23, 2011
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 50 Issue 3

Abstract

Background: Dysregulation of antimicrobial response may trigger inflammatory bowel diseases (IBD). This study analyzed specificity of anti-neutrophil cytoplasmic antibodies (ANCA) in IBD patients and its clinical significance.

Methods: Data from 52 ulcerative colitis (UC) patients with 32 Crohn’s disease (CD) patients were compared. Primary sclerosing cholangitis (PSC) was present in 12/84 patients. ANCA, ANA and anti-smooth muscle antibodies (ASMA) were detected by IIF. ANCA were tested by ELISA for proteinase 3 (PR3), myeloperoxidase, bactericidal/permeability increasing protein, elastase, cathepsin G, lysozyme and lactoferrin.

Results: pANCA were more frequently present in UC than in CD patients (p<0.001). ANCA titer correlated with the disease activity only in UC patients (p<0.05). UC patients more frequently had two or more ANCA specificities compared to CD patients (p<0.01). Multi-specific ANCA in medium and/or high concentrations were associated with long-lasting (p<0.05) and left-sided UC (p<0.001). Multi-specific ANCA with ANA and ASMA had sensitivity of 67% for PSC.

Conclusions: Higher concentrations of multi-specific ANCA in long-lasting, left-sided UC suggest an influence of bacterial stimulation on the break of tolerance. Multi-specific ANCA with ANA and ASMA could be markers for PSC. ANCA specific to several antigens may worsen inflammation by reducing antimicrobial capacity of neutrophil proteases and cationic proteins.

Keywords: anti-neutrophil cytoplasmic antibodies (ANCA); anti-nuclear antibodies; anti-smooth muscle antibodies; bactericidal/permeability increasing-ANCA; primary sclerosing cholangitis; ulcerative colitis
Corresponding author: Branka Bonaci-Nikolic, MD, PhD, Department of Allergy and Clinical Immunology, Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Koste Todorovica 2, 11000 Belgrade, Serbia Phone: +381 11 66 8300 407, Fax: +381 11 2682 652
Received: 2011-7-13
Accepted: 2011-10-26
Published Online: 2011-11-23
Published in Print: 2012-03-01

©2012 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm.2011.797
Keywords for this article
anti-neutrophil cytoplasmic antibodies (ANCA); anti-nuclear antibodies; anti-smooth muscle antibodies; bactericidal/permeability increasing-ANCA; primary sclerosing cholangitis; ulcerative colitis

Articles in the same Issue

  1. Editorials
  2. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports
  3. Cancer diagnosis: from dogs to DNA or from DNA to dogs?
  4. The never-ending search of an acceptable compromise for pancreatic lipase standardisation
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  9. Genetics and Molecular Diagnostics
  10. SLCO1B1 gene variability influences lipid-lowering efficacy on simvastatin therapy in Southern Brazilians
  11. Elevated plasma matrix metalloproteinase-9 protein and its gene polymorphism in patients with community-acquired pneumonia
  12. General Clinical Chemistry and Laboratory Medicine
  13. Minimum analytical quality specifications of inter-laboratory comparisons: agreement among Spanish EQAP organizers
  14. Monitoring quality indicators in laboratory medicine does not automatically result in quality improvement
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