Artikel
Lizenziert
Nicht lizenziert
Erfordert eine Authentifizierung
Evaluation of cell counting and leukocyte differentiation in cerebrospinal fluid controls using hematology analyzers by the German Society for Clinical Chemistry and Laboratory Medicine
-
Tilmann O. Kleine
Veröffentlicht/Copyright:
4. Mai 2010
Abstract
Background: Manual cell counting in cerebrospinal fluid (CSF) is technique-dependent, time-consuming, and thus costly and prone to inter-operator variability and low precision. Flow cytometry (FCM) with fast hematology analyzers (HAs) appears to improve accuracy and precision of CSF cell analysis; rapid CSF cell analysis is especially needed in emergency laboratories. Ten external trials of the German Society for Clinical Chemistry and Laboratory Medicine evaluated FCM with Coulter (LH750, 755), Abbott CD3200™, CD3500™, CD3700™, CD4000™, Sapphire™, ADVIA120® CSF assay, and Sysmex® XE-2100 single platform analyzers.
Methods: CSF controls were produced using native blood leukocytes and erythrocytes, resembling CSF and thus rendering the trials feasible and allowing comparison with native manual counting in a Fuchs-Rosenthal chamber and FACScan-CD45-CD14 dual platform analysis, which was used as the reference method. Statistical evaluation was performed using Passing/Bablok regression analysis.
Results: Our evaluation revealed significant differences with respect to target values in leukocyte and erythrocyte counts, as well as leukocyte differentiation. These differences were attributed to inaccuracies produced by the HAs, due to blank correction in connection with impedance analysis, leukocyte loss, especially through monocyte injury due to the erythrocyte lysing agent, incomplete erythrocyte lysis, ADVIA cell sphering, cell differentiation using algorithms and peroxidase activity. Erythrocyte counting in the CSF controls was inaccurate with the Coulter and ADVIA analyzers.
Conclusions: Evaluation of HAs by means of the CSF controls revealed inaccuracies in cell counting and leukocyte differentiation. Analyzer techniques, used for CSF cell assays, therefore need to be improved.
Clin Chem Lab Med 2010;48:839–48.
Keywords: cerebrospinal fluid cell analysis; chamber cell counting; flow cytometry; hematology analyzer
Received: 2009-6-16
Accepted: 2010-2-3
Published Online: 2010-05-4
Published in Print: 2010-06-01
©2010 by Walter de Gruyter Berlin New York
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Artikel in diesem Heft
- Editorials
- The “Obamanomics”: a revolution in laboratory diagnostics
- Skeletal muscle biomarkers: not new but still interesting diagnostic tools
- Necessity for high-quality reference materials in the harmonization of laboratory assays
- Reviews
- Rhabdomyolysis: historical background, clinical, diagnostic and therapeutic features
- Biochemical markers of muscular damage
- Minireview
- Interpretation of serum parathyroid hormone concentrations in dialysis patients: what do the KDIGO guidelines change for the clinical laboratory?
- Guidelines and Recommendations
- 2010 Consensus statement on the worldwide standardization of the hemoglobin A1c measurement
- Genetics and Molecular Diagnostics
- Development of a low-cost real-time reverse transcriptase-polymerase chain reaction technique for the detection and quantification of hepatitis C viral load
- Microsatellite polymorphisms in intron 2 of the toll-like receptor 2 gene and their association with susceptibility to pulmonary tuberculosis in Han Chinese
- Evaluation of a panel of circulating DNA, RNA and protein potential markers for pathologies of pregnancy
- General Clinical Chemistry and Laboratory Medicine
- Traceability of values for catalytic activity concentration of enzymes: a Certified Reference Material for aspartate transaminase
- Development and preparation of a new serum protein reference material: feasibility studies and processing
- A WHO Reference Reagent for the Serum Transferrin Receptor (sTfR): international collaborative study to evaluate a recombinant soluble transferrin receptor preparation
- Hyperhomocysteinemia is not associated with reduced bone quality in humans with hip osteoarthritis
- Diagnostic performance of anti-citrullinated peptide antibodies for the diagnosis of rheumatoid arthritis: the relevance of likelihood ratios
- New quantitative electrochemiluminescence method (ECLIA) for interleukin-6 (IL-6) measurement
- Evaluation of cell counting and leukocyte differentiation in cerebrospinal fluid controls using hematology analyzers by the German Society for Clinical Chemistry and Laboratory Medicine
- Reference Values and Biological Variations
- The relationship of coffee and green tea consumption with high-sensitivity C-reactive protein in Japanese men and women
- Cancer Diagnostics
- Potential biomarkers for esophageal carcinoma detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- Identification of candidate molecular markers predicting chemotherapy resistance in non-small cell lung cancer
- Factors affecting soluble mesothelin related protein levels in an asbestos-exposed population
- Cardiovascular Diseases
- Dynamic changes in N-terminal pro-brain natriuretic peptide in acute coronary syndromes treated with percutaneous coronary intervention: a marker of ischemic burden, reperfusion and outcome
- Performance characteristics of a microfluidic Lab-on-Chip electrophoresis system for high-density lipoprotein (HDL) subfraction separation and measurement
- Letters to the Editor
- Hemolysis correction factor in the measurement of serum neuron-specific enolase
- Serum paraoxonase-1 activity and genetic polymorphisms: common errors in measurement and interpretation of results
- Neuropharmacology of schizophrenia: is there a role for leptin?
- Relationship between serum concentrations of keratin-18 and apoptosis in chronic hepatitis C
Schlagwörter für diesen Artikel
cerebrospinal fluid cell analysis;
chamber cell counting;
flow cytometry;
hematology analyzer
Artikel in diesem Heft
- Editorials
- The “Obamanomics”: a revolution in laboratory diagnostics
- Skeletal muscle biomarkers: not new but still interesting diagnostic tools
- Necessity for high-quality reference materials in the harmonization of laboratory assays
- Reviews
- Rhabdomyolysis: historical background, clinical, diagnostic and therapeutic features
- Biochemical markers of muscular damage
- Minireview
- Interpretation of serum parathyroid hormone concentrations in dialysis patients: what do the KDIGO guidelines change for the clinical laboratory?
- Guidelines and Recommendations
- 2010 Consensus statement on the worldwide standardization of the hemoglobin A1c measurement
- Genetics and Molecular Diagnostics
- Development of a low-cost real-time reverse transcriptase-polymerase chain reaction technique for the detection and quantification of hepatitis C viral load
- Microsatellite polymorphisms in intron 2 of the toll-like receptor 2 gene and their association with susceptibility to pulmonary tuberculosis in Han Chinese
- Evaluation of a panel of circulating DNA, RNA and protein potential markers for pathologies of pregnancy
- General Clinical Chemistry and Laboratory Medicine
- Traceability of values for catalytic activity concentration of enzymes: a Certified Reference Material for aspartate transaminase
- Development and preparation of a new serum protein reference material: feasibility studies and processing
- A WHO Reference Reagent for the Serum Transferrin Receptor (sTfR): international collaborative study to evaluate a recombinant soluble transferrin receptor preparation
- Hyperhomocysteinemia is not associated with reduced bone quality in humans with hip osteoarthritis
- Diagnostic performance of anti-citrullinated peptide antibodies for the diagnosis of rheumatoid arthritis: the relevance of likelihood ratios
- New quantitative electrochemiluminescence method (ECLIA) for interleukin-6 (IL-6) measurement
- Evaluation of cell counting and leukocyte differentiation in cerebrospinal fluid controls using hematology analyzers by the German Society for Clinical Chemistry and Laboratory Medicine
- Reference Values and Biological Variations
- The relationship of coffee and green tea consumption with high-sensitivity C-reactive protein in Japanese men and women
- Cancer Diagnostics
- Potential biomarkers for esophageal carcinoma detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- Identification of candidate molecular markers predicting chemotherapy resistance in non-small cell lung cancer
- Factors affecting soluble mesothelin related protein levels in an asbestos-exposed population
- Cardiovascular Diseases
- Dynamic changes in N-terminal pro-brain natriuretic peptide in acute coronary syndromes treated with percutaneous coronary intervention: a marker of ischemic burden, reperfusion and outcome
- Performance characteristics of a microfluidic Lab-on-Chip electrophoresis system for high-density lipoprotein (HDL) subfraction separation and measurement
- Letters to the Editor
- Hemolysis correction factor in the measurement of serum neuron-specific enolase
- Serum paraoxonase-1 activity and genetic polymorphisms: common errors in measurement and interpretation of results
- Neuropharmacology of schizophrenia: is there a role for leptin?
- Relationship between serum concentrations of keratin-18 and apoptosis in chronic hepatitis C
