Article
Licensed
Unlicensed
Requires Authentication
Potential biomarkers for esophageal carcinoma detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
-
Li-Hua Liu
Published/Copyright:
March 26, 2010
Abstract
Background: Currently, no satisfactory biomarkers are available to screen for esophageal squamous cell carcinoma (ESCC). The goal of this study was to find biomarkers and establish a serum protein fingerprint model for early diagnosis of ESCC using the ClinProt protocol of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Methods: Serum samples were collected from 62 patients with ESCC, nine patients with esophageal adenocarcinoma (EA) and 38 healthy individuals. Proteomic spectra of mass to charge ratio (m/z) were generated following the application of plasma to weak cationic-exchanger magnetic beads (WCX-MB). The spectral data were analyzed using a support vector machine, and potential biomarkers were chosen for system training and used to construct diagnostic models.
Results: Three differential patterns were established using MALDI-TOF MS. Pattern 1, consisting of 11 protein peaks, separated ESCC patients from the healthy individuals with a sensitivity of 90.0% and a specificity of 88.4%. Pattern 2, consisting of eight protein peaks, separated ESCC in stage I and stage II from stage III and stage IV with a sensitivity of 92.9% and a specificity of 82.3%. Pattern 3, consisting of seven protein peaks, separated ESCC from EA with a sensitivity of 91.3% and a specificity of 80.0%.
Conclusions: These results suggested that MALDI-TOF MS combined with MB separation yields significantly higher sensitivity and specificity for the detection of serum protein in patients with ESCC.
Clin Chem Lab Med 2010;48:855–61.
Received: 2009-10-6
Accepted: 2010-1-12
Published Online: 2010-03-26
Published in Print: 2010-06-01
©2010 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorials
- The “Obamanomics”: a revolution in laboratory diagnostics
- Skeletal muscle biomarkers: not new but still interesting diagnostic tools
- Necessity for high-quality reference materials in the harmonization of laboratory assays
- Reviews
- Rhabdomyolysis: historical background, clinical, diagnostic and therapeutic features
- Biochemical markers of muscular damage
- Minireview
- Interpretation of serum parathyroid hormone concentrations in dialysis patients: what do the KDIGO guidelines change for the clinical laboratory?
- Guidelines and Recommendations
- 2010 Consensus statement on the worldwide standardization of the hemoglobin A1c measurement
- Genetics and Molecular Diagnostics
- Development of a low-cost real-time reverse transcriptase-polymerase chain reaction technique for the detection and quantification of hepatitis C viral load
- Microsatellite polymorphisms in intron 2 of the toll-like receptor 2 gene and their association with susceptibility to pulmonary tuberculosis in Han Chinese
- Evaluation of a panel of circulating DNA, RNA and protein potential markers for pathologies of pregnancy
- General Clinical Chemistry and Laboratory Medicine
- Traceability of values for catalytic activity concentration of enzymes: a Certified Reference Material for aspartate transaminase
- Development and preparation of a new serum protein reference material: feasibility studies and processing
- A WHO Reference Reagent for the Serum Transferrin Receptor (sTfR): international collaborative study to evaluate a recombinant soluble transferrin receptor preparation
- Hyperhomocysteinemia is not associated with reduced bone quality in humans with hip osteoarthritis
- Diagnostic performance of anti-citrullinated peptide antibodies for the diagnosis of rheumatoid arthritis: the relevance of likelihood ratios
- New quantitative electrochemiluminescence method (ECLIA) for interleukin-6 (IL-6) measurement
- Evaluation of cell counting and leukocyte differentiation in cerebrospinal fluid controls using hematology analyzers by the German Society for Clinical Chemistry and Laboratory Medicine
- Reference Values and Biological Variations
- The relationship of coffee and green tea consumption with high-sensitivity C-reactive protein in Japanese men and women
- Cancer Diagnostics
- Potential biomarkers for esophageal carcinoma detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- Identification of candidate molecular markers predicting chemotherapy resistance in non-small cell lung cancer
- Factors affecting soluble mesothelin related protein levels in an asbestos-exposed population
- Cardiovascular Diseases
- Dynamic changes in N-terminal pro-brain natriuretic peptide in acute coronary syndromes treated with percutaneous coronary intervention: a marker of ischemic burden, reperfusion and outcome
- Performance characteristics of a microfluidic Lab-on-Chip electrophoresis system for high-density lipoprotein (HDL) subfraction separation and measurement
- Letters to the Editor
- Hemolysis correction factor in the measurement of serum neuron-specific enolase
- Serum paraoxonase-1 activity and genetic polymorphisms: common errors in measurement and interpretation of results
- Neuropharmacology of schizophrenia: is there a role for leptin?
- Relationship between serum concentrations of keratin-18 and apoptosis in chronic hepatitis C
Articles in the same Issue
- Editorials
- The “Obamanomics”: a revolution in laboratory diagnostics
- Skeletal muscle biomarkers: not new but still interesting diagnostic tools
- Necessity for high-quality reference materials in the harmonization of laboratory assays
- Reviews
- Rhabdomyolysis: historical background, clinical, diagnostic and therapeutic features
- Biochemical markers of muscular damage
- Minireview
- Interpretation of serum parathyroid hormone concentrations in dialysis patients: what do the KDIGO guidelines change for the clinical laboratory?
- Guidelines and Recommendations
- 2010 Consensus statement on the worldwide standardization of the hemoglobin A1c measurement
- Genetics and Molecular Diagnostics
- Development of a low-cost real-time reverse transcriptase-polymerase chain reaction technique for the detection and quantification of hepatitis C viral load
- Microsatellite polymorphisms in intron 2 of the toll-like receptor 2 gene and their association with susceptibility to pulmonary tuberculosis in Han Chinese
- Evaluation of a panel of circulating DNA, RNA and protein potential markers for pathologies of pregnancy
- General Clinical Chemistry and Laboratory Medicine
- Traceability of values for catalytic activity concentration of enzymes: a Certified Reference Material for aspartate transaminase
- Development and preparation of a new serum protein reference material: feasibility studies and processing
- A WHO Reference Reagent for the Serum Transferrin Receptor (sTfR): international collaborative study to evaluate a recombinant soluble transferrin receptor preparation
- Hyperhomocysteinemia is not associated with reduced bone quality in humans with hip osteoarthritis
- Diagnostic performance of anti-citrullinated peptide antibodies for the diagnosis of rheumatoid arthritis: the relevance of likelihood ratios
- New quantitative electrochemiluminescence method (ECLIA) for interleukin-6 (IL-6) measurement
- Evaluation of cell counting and leukocyte differentiation in cerebrospinal fluid controls using hematology analyzers by the German Society for Clinical Chemistry and Laboratory Medicine
- Reference Values and Biological Variations
- The relationship of coffee and green tea consumption with high-sensitivity C-reactive protein in Japanese men and women
- Cancer Diagnostics
- Potential biomarkers for esophageal carcinoma detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- Identification of candidate molecular markers predicting chemotherapy resistance in non-small cell lung cancer
- Factors affecting soluble mesothelin related protein levels in an asbestos-exposed population
- Cardiovascular Diseases
- Dynamic changes in N-terminal pro-brain natriuretic peptide in acute coronary syndromes treated with percutaneous coronary intervention: a marker of ischemic burden, reperfusion and outcome
- Performance characteristics of a microfluidic Lab-on-Chip electrophoresis system for high-density lipoprotein (HDL) subfraction separation and measurement
- Letters to the Editor
- Hemolysis correction factor in the measurement of serum neuron-specific enolase
- Serum paraoxonase-1 activity and genetic polymorphisms: common errors in measurement and interpretation of results
- Neuropharmacology of schizophrenia: is there a role for leptin?
- Relationship between serum concentrations of keratin-18 and apoptosis in chronic hepatitis C
