Article
Licensed
Unlicensed
Requires Authentication
The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin
-
Brendan Byrne
Published/Copyright:
November 30, 2009
Abstract
Background: Commercially available prolactin immunoassays detect macroprolactin to variable degrees. Best practice requires laboratories to assess the cross-reactivity of their prolactin assay with macroprolactin, and where appropriate, introduce a screen for the presence of macroprolactin. Our policy has been to reanalyse hyperprolactinaemic samples following polyethylene glycol (PEG) precipitation and to report the resultant value as the monomeric prolactin content of the sample. The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800.
Methods: A total of 317 apparently hyperprolactinaemic samples were analysed for prolactin using the Beckman DxI 800 and results compared with those determined with the PEG screening technique on the Wallac AutoDELFIA. Any samples demonstrating a discordance of >25% were re-analysed using gel filtration chromatography (GFC) for a definitive result.
Results: The results indicate the Beckman DxI overestimates the prolactin concentration in 1%–2% of hyperprolactinaemic samples. PEG precipitation prior to analysis with the Wallac AutoDELFIA resulted in a 1% false negative diagnosis of hyperprolactinaemia.
Conclusions: Prolactin results can be reported directly from the DxI. When results are discordant with the clinical presentation, prolactin should be measured using GFC.
Clin Chem Lab Med 2010;48:205–8.
Received: 2009-7-20
Accepted: 2009-10-2
Published Online: 2009-11-30
Published in Print: 2010-02-01
©2010 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Review
- Elevated serum γ-glutamyltransferase activity is associated with increased risk of mortality, incident type 2 diabetes, cardiovascular events, chronic kidney disease and cancer – a narrative review
- Minireview
- Artificial intelligence for diagnostic purposes: principles, procedures and limitations
- General Clinical Chemistry and Laboratory Medicine
- Association between serum alkaline phosphatase and C-reactive protein in the United States National Health and Nutrition Examination Survey 2005–2006
- Association between serum uric acid and non-alcoholic fatty liver disease in Korean adults
- Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?
- Determination of globotriaosylceramide in plasma and urine by mass spectrometry
- Effect of inflammation induced by prolonged exercise on circulating erythroid progenitors and markers of erythropoiesis
- The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin
- Stability of cerebrospinal fluid/serum glucose ratio and cerebrospinal fluid lactate concentrations over 24 h: analysis of repeated measurements
- O-β-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots
- Validation and Outcome Studies
- Experimental validation of specificity of the squamous cell carcinoma antigen-immunoglobulin M (SCCA-IgM) assay in patients with cirrhosis
- Performance analysis of the ARCHITECT anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis
- Reference Values and Biological Variations
- Applicability of common reference intervals for serum creatinine concentrations to the Croatian population
- Laboratory reference intervals during pregnancy, delivery and the early postpartum period
- Determination of serum holotranscobalamin concentrations with the AxSYM active B12 assay: cut-off point evaluation in the clinical laboratory
- Definition of reference ranges for the platelet distribution width (PDW): a local need
- Cancer Diagnostics
- Mean leukocyte telomere length and risk of incident colorectal carcinoma in women: a prospective, nested case-control study
- Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma
- Soluble mesothelin related peptides (SMRP) and osteopontin as protein biomarkers for malignant mesothelioma: analytical validation of ELISA based assays and characterization at mRNA and protein levels
- A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen
- Prostate cancer screening: clinical impact of WHO calibration of Beckman Coulter Access® prostate-specific antigen assays
- Infectious Diseases
- Use of flow cytometry (Sysmex® UF-100) to screen for positive urine cultures: in search for the ideal cut-off
- Letters to the Editor
- Improving laboratory efficiencies through significant time reduction in the preanalytical phase
- Comparison of the Vitek 2 system with the CLSI broth microdilution, disk diffusion, and sterol quantitation methods for determining fluconazole susceptibility against Candida spp.
Keywords for this article
hyperprolactinaemia;
macroprolactin;
macroprolactinaemia;
screening;
prolactin
Articles in the same Issue
- Review
- Elevated serum γ-glutamyltransferase activity is associated with increased risk of mortality, incident type 2 diabetes, cardiovascular events, chronic kidney disease and cancer – a narrative review
- Minireview
- Artificial intelligence for diagnostic purposes: principles, procedures and limitations
- General Clinical Chemistry and Laboratory Medicine
- Association between serum alkaline phosphatase and C-reactive protein in the United States National Health and Nutrition Examination Survey 2005–2006
- Association between serum uric acid and non-alcoholic fatty liver disease in Korean adults
- Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?
- Determination of globotriaosylceramide in plasma and urine by mass spectrometry
- Effect of inflammation induced by prolonged exercise on circulating erythroid progenitors and markers of erythropoiesis
- The Beckman DxI 800 prolactin assay demonstrates superior specificity for monomeric prolactin
- Stability of cerebrospinal fluid/serum glucose ratio and cerebrospinal fluid lactate concentrations over 24 h: analysis of repeated measurements
- O-β-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots
- Validation and Outcome Studies
- Experimental validation of specificity of the squamous cell carcinoma antigen-immunoglobulin M (SCCA-IgM) assay in patients with cirrhosis
- Performance analysis of the ARCHITECT anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis
- Reference Values and Biological Variations
- Applicability of common reference intervals for serum creatinine concentrations to the Croatian population
- Laboratory reference intervals during pregnancy, delivery and the early postpartum period
- Determination of serum holotranscobalamin concentrations with the AxSYM active B12 assay: cut-off point evaluation in the clinical laboratory
- Definition of reference ranges for the platelet distribution width (PDW): a local need
- Cancer Diagnostics
- Mean leukocyte telomere length and risk of incident colorectal carcinoma in women: a prospective, nested case-control study
- Heat shock protein 27 is over-expressed in tumor tissues and increased in sera of patients with gastric adenocarcinoma
- Soluble mesothelin related peptides (SMRP) and osteopontin as protein biomarkers for malignant mesothelioma: analytical validation of ELISA based assays and characterization at mRNA and protein levels
- A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen
- Prostate cancer screening: clinical impact of WHO calibration of Beckman Coulter Access® prostate-specific antigen assays
- Infectious Diseases
- Use of flow cytometry (Sysmex® UF-100) to screen for positive urine cultures: in search for the ideal cut-off
- Letters to the Editor
- Improving laboratory efficiencies through significant time reduction in the preanalytical phase
- Comparison of the Vitek 2 system with the CLSI broth microdilution, disk diffusion, and sterol quantitation methods for determining fluconazole susceptibility against Candida spp.
