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Hypoxia related growth factors and p53 in preoperative sera from patients with colorectal cancer – evaluation of the prognostic significance of these agents

  • Stanislaw Sulkowski , Andrzej Wincewicz , Bogdan Zalewski , Waldemar Famulski , Joanna Maria Lotowska , Mariusz Koda , Maria Elzbieta Sobaniec-Lotowska , Michal Mysliwiec , Marek Baltaziak , Krystyna Pawlak and Mariola Sulkowska
Published/Copyright: October 12, 2009
Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 47 Issue 11

Abstract

Background: Insulin-like growth factor-I (IGF-I) and vascular endothelial growth factor (VEGF) belong to a group of hypoxia related proteins. IGF-I induces expression of VEGF and decomposes wild type p53 in cancer cell lines. The goal of our study was to evaluate serum IGF-I, VEGF and p53 with respect to overall and disease free survival of patients with colorectal cancer (CRC) patients compared with healthy volunteers.

Methods: Preoperative blood samples from 125 patients with CRC and 16 healthy volunteers were examined using ELISA for serum IGF-I, p53 and VEGF concentrations.

Results: Concentrations of p53 and VEGF were significantly higher in CRC patients than in controls (p<0.0006 and p<0.0001, respectively). IGF-I was not statistically different between both groups. Serum IGF-I showed negative correlation with p53 in CRC patients (p<0.04, r=−0.193). IGF-I and VEGF showed negative correlation in poorly differentiated cancers (G3) (p<0.03, r=−0.339). Patients with VEGF concentrations that were above average for the cancer population survived for a shorter period of time (p=0.065 in evaluation of overall survival and 0.071 in estimation of disease-free survival during a 3-year follow-up) compared with patients with serum VEGF lower than the highest values seen in controls.

Conclusions: Comparisons between serum IGF-I and p53 appear to confirm the metabolism of p53 by IGF-I. Serum VEGF showed prognostic significance in our study. Serum concentrations of IGF-I and VEGF did not show positive correlation, as expected due to IGF-I induction of VEGF in malignant colon cell lines.

Clin Chem Lab Med 2009;47:1439–45.

Keywords: colorectal cancer; insulin-like growth factor-I; p53; serum; vascular endothelial growth factor
Corresponding author: Andrzej Wincewicz, MD, PhD, Department of Medical Pathomorphology, Medical University of Bialystok, Waszyngtona St 13, 15-269 Bialystok, Poland Phone: +4885 748 59 45, Fax: +4885 748 59 44,
Received: 2009-6-8
Accepted: 2009-7-22
Published Online: 2009-10-12
Published in Print: 2009-11-01

©2009 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2009.305
Keywords for this article
colorectal cancer; insulin-like growth factor-I; p53; serum; vascular endothelial growth factor

Articles in the same Issue

  1. Editorial
  2. The journal impact factor: navigating between Scylla and Charybdis
  3. Opinion Papers
  4. Journal Impact Factor: it will go away soon
  5. The Journal Impact Factor: don't expect its demise any time soon
  6. Reviews
  7. Discordant total and free prostate-specific antigen (PSA) assays: does calibration with WHO reference materials diminish the problem?
  8. Molecular diagnostics in acute leukemias
  9. Opinion Paper
  10. Considerations for the development of a reference method for sequencing of haploid DNA – an opinion paper on behalf of the IFCC Committee on Molecular Diagnostics. International Federation of Clinical Chemistry and Laboratory Medicine
  11. General Clinical Chemistry and Laboratory Medicine
  12. Meta-analyses of diagnostic studies
  13. Development and implementation of an automatic system for verification, validation and delivery of laboratory test results
  14. Comparison of visual vs. automated detection of lipemic, icteric and hemolyzed specimens: can we rely on a human eye?
  15. Prevalence of thyroid autoimmunity and subclinical hypothyroidism in persons with chronic kidney disease not requiring chronic dialysis
  16. Serum albumin fragmentation in end-stage renal disease patients – a pilot study
  17. Enhanced bone metabolism in vegetarians – the role of vitamin B12 deficiency
  18. Impaired anti-inflammatory function of apolipoprotein A-II concentrations predicts metabolic syndrome and diabetes at 4 years follow-up in elderly Turks
  19. Detection of myelin autoantibodies: evaluation of an assay system for diagnosis of multiple sclerosis in differentiation from other central nervous system diseases
  20. The rate of manual peripheral blood smear reviews in outpatients
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  28. Hypoxia related growth factors and p53 in preoperative sera from patients with colorectal cancer – evaluation of the prognostic significance of these agents
  29. Letters to the Editor
  30. Quick and easy colorimetric test for calibration control of liquid handling workstations
  31. GSTP1*B allele increases the risk for asthma in children
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