–308G>A and –1031T>C tumor necrosis factor gene polymorphisms in Tunisian patients with coronary artery disease
-
Lakhdar Ghazouani
Abstract
Background: Recent research has shown that inflammation plays a key role in coronary artery disease (CAD) and other manifestations of atherosclerosis. Several lines of evidence support a key role for tumor necrosis factor-α (TNF-α), a potent immunomodulator and pro-inflammatory cytokine, in the development of atherosclerosis and in complications of CAD.
Methods: We investigated the possible association between CAD and the TNF gene promoter polymorphisms –308G>A and –1031T>C in a Tunisian population. We compared the distribution of these polymorphisms between 418 patients with CAD and 406 healthy controls using polymerase chain reaction restriction fragment length-polymorphism analysis.
Results: The frequency of the TNF-α –308A allele in the control group was similar to that observed in CAD patients [p=0.78; odds ratio (OR)=1.15; 95% confidence interval (CI)=0.86–1.55], but higher than those described in other Europeans, such as in the French, Finnish and Spanish. Concerning the TNF-α –1031T/C polymorphism, the same distribution was observed between patients with CAD and controls (p=0.12; OR=1.27; 95% CI=0.94–1.72). In addition, the genotype and allele frequencies of control individuals were comparable to those previously reported in healthy Tunisian controls and other ethnic groups. Haplotype analysis (TNF-α –308G>A and –1031T>C) demonstrated no significant association between TNF haplotypes and CAD.
Conclusions: We conclude that TNF promoter gene polymorphisms at position –308G>A and –1031T>C do not play a major role in the pathogenesis of CAD in the Tunisian population.
Clin Chem Lab Med 2009;47:1247–51.
©2009 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Editorial
- Cardiac troponins: what we knew, what we know – where are we now?
- Review
- Measurement of circulating concentrations of cardiac troponin I and T in healthy subjects: a tool for monitoring myocardial tissue renewal?
- Opinion Papers
- A critical appraisal of experimental factors influencing the definition of the 99th percentile limit for cardiac troponins
- Genetic and biochemical heterogeneity of cardiac troponins: clinical and laboratory implications
- The future of hospital laboratories. Position statement from the Royal Belgian Society of Clinical Chemistry (RBSCC)
- Point
- Quantity quotient reporting. A proposal for a standardized presentation of laboratory results
- Counterpoint
- Quantity quotient reporting. Counterpoint
- Perspectives
- Increasing laboratory medicine activities in China: research and publications
- Contributions in Biochemistry and Molecular Biology from China and other top-ranking countries: a 10-year survey of the literature
- Genetics and Molecular Diagnostics
- SOCS3 and IRS-1 gene expression differs between genotype 1 and genotype 2 hepatitis C virus-infected HepG2 cells
- Polymorphisms of the β1-adrenergic receptor gene are associated with essential hypertension in Chinese
- Egyptian glycogen storage disease type III – identification of six novel AGL mutations, including a large 1.5 kb deletion and a missense mutation p.L620P with subtype IIId
- Novel rare alleles of ABCA1 are exclusively associated with extreme high-density lipoprotein-cholesterol levels among the Han Chinese
- –308G>A and –1031T>C tumor necrosis factor gene polymorphisms in Tunisian patients with coronary artery disease
- General Clinical Chemistry and Laboratory Medicine
- Error tracking in a clinical biochemistry laboratory
- HbA1c measurements from dried blood spots: validation and patient satisfaction
- Pre-transplant serum concentrations of anti-endothelial cell antibody in panel reactive antibody negative renal recipients and its impact on acute rejection
- Cancer Diagnostics
- Detection of Meningeosis neoplastica by real-time quantitation of telomerase activity
- The prognostic utility of haptoglobin genotypes in squamous cell carcinoma of the head and neck
- Evaluation of a new method for the diagnosis of alterations of Lens culinaris agglutinin binding of thyroglobulin molecules in thyroid carcinoma
- The soluble transferrin receptor (TfR)-F-Index is not applicable as a test for iron status in patients with chronic lymphocytic leukemia
- Impact of calibration fitting models on the clinical value of chromogranin A
- Letters to the Editor
- Clinical value of a competitive NT-proBNP enzyme immunoassay compared to the Roche NT-proBNP platform
- Should gender-related reference values be used for total bilirubin?
- A novel nonsense mutation in the albumin gene (c.1275 C>A) causing analbuminemia in a Tunisian boy
- Abstracts
- 41st National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, 2nd Joint National Event SIBioC-SIMeL
Articles in the same Issue
- Editorial
- Cardiac troponins: what we knew, what we know – where are we now?
- Review
- Measurement of circulating concentrations of cardiac troponin I and T in healthy subjects: a tool for monitoring myocardial tissue renewal?
- Opinion Papers
- A critical appraisal of experimental factors influencing the definition of the 99th percentile limit for cardiac troponins
- Genetic and biochemical heterogeneity of cardiac troponins: clinical and laboratory implications
- The future of hospital laboratories. Position statement from the Royal Belgian Society of Clinical Chemistry (RBSCC)
- Point
- Quantity quotient reporting. A proposal for a standardized presentation of laboratory results
- Counterpoint
- Quantity quotient reporting. Counterpoint
- Perspectives
- Increasing laboratory medicine activities in China: research and publications
- Contributions in Biochemistry and Molecular Biology from China and other top-ranking countries: a 10-year survey of the literature
- Genetics and Molecular Diagnostics
- SOCS3 and IRS-1 gene expression differs between genotype 1 and genotype 2 hepatitis C virus-infected HepG2 cells
- Polymorphisms of the β1-adrenergic receptor gene are associated with essential hypertension in Chinese
- Egyptian glycogen storage disease type III – identification of six novel AGL mutations, including a large 1.5 kb deletion and a missense mutation p.L620P with subtype IIId
- Novel rare alleles of ABCA1 are exclusively associated with extreme high-density lipoprotein-cholesterol levels among the Han Chinese
- –308G>A and –1031T>C tumor necrosis factor gene polymorphisms in Tunisian patients with coronary artery disease
- General Clinical Chemistry and Laboratory Medicine
- Error tracking in a clinical biochemistry laboratory
- HbA1c measurements from dried blood spots: validation and patient satisfaction
- Pre-transplant serum concentrations of anti-endothelial cell antibody in panel reactive antibody negative renal recipients and its impact on acute rejection
- Cancer Diagnostics
- Detection of Meningeosis neoplastica by real-time quantitation of telomerase activity
- The prognostic utility of haptoglobin genotypes in squamous cell carcinoma of the head and neck
- Evaluation of a new method for the diagnosis of alterations of Lens culinaris agglutinin binding of thyroglobulin molecules in thyroid carcinoma
- The soluble transferrin receptor (TfR)-F-Index is not applicable as a test for iron status in patients with chronic lymphocytic leukemia
- Impact of calibration fitting models on the clinical value of chromogranin A
- Letters to the Editor
- Clinical value of a competitive NT-proBNP enzyme immunoassay compared to the Roche NT-proBNP platform
- Should gender-related reference values be used for total bilirubin?
- A novel nonsense mutation in the albumin gene (c.1275 C>A) causing analbuminemia in a Tunisian boy
- Abstracts
- 41st National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, 2nd Joint National Event SIBioC-SIMeL
