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HbA1c measurements from dried blood spots: validation and patient satisfaction

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Published/Copyright: September 14, 2009
Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 47 Issue 10

Abstract

Background: This study evaluates HbA1c measurements from dried blood spots collected on filter paper and compares HbA1c from filter paper (capillary blood) with HbA1c measured in venous blood.

Methods: Patient satisfaction was evaluated using a questionnaire. The performance with the filter paper method was assessed by comparing HbA1c results from EDTA-blood samples obtained via dried blood spots with HbA1c results obtained with freshly hemolyzed blood (routine HbA1c). Adult patients visiting the outpatient clinic for HbA1c analyses were recruited for the evaluation of dried blood spot sampling at home. Laboratory personnel collected a capillary blood sample on filter paper as well as a venous EDTA-blood sample. The participants collected another capillary blood sample at home and sent the dried filter paper back to the laboratory. Samples were analyzed with an immunoturbidimetric assay.

Results: Between-filter coefficient of variation was 1.8%. Filter paper HbA1c increased slightly during storage, particularly during the first 5 days. Filter paper HbA1c highly correlated with routine HbA1c (r=0.987). The evaluation of samples collected at home showed comparable HbA1c values by filter paper and routine sampling methods (n=93). Eighty-three percent of participants said they would like the filter method to be brought into practice.

Conclusions: Home HbA1c sampling on filter paper is an acceptable sampling alternative for analysis of HbA1c.

Clin Chem Lab Med 2009;47:1259–64.

Keywords: capillary blood; dried blood spots; filter paper; HbA1c; patient satisfaction
Corresponding author: Dr. M.R. Fokkema, Universitair Medisch Centrum Groningen, Department of Laboratory Medicine, CMC-V, Room Y2.119, HPA EA61, Hanzeplein 1, 9713 GZ Groningen, The Netherlands Phone: +31 50 3615118,
Received: 2009-3-12
Accepted: 2009-7-6
Published Online: 2009-09-14
Published in Print: 2009-10-01

©2009 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2009.274
Keywords for this article
capillary blood; dried blood spots; filter paper; HbA1c; patient satisfaction

Articles in the same Issue

  1. Editorial
  2. Cardiac troponins: what we knew, what we know – where are we now?
  3. Review
  4. Measurement of circulating concentrations of cardiac troponin I and T in healthy subjects: a tool for monitoring myocardial tissue renewal?
  5. Opinion Papers
  6. A critical appraisal of experimental factors influencing the definition of the 99th percentile limit for cardiac troponins
  7. Genetic and biochemical heterogeneity of cardiac troponins: clinical and laboratory implications
  8. The future of hospital laboratories. Position statement from the Royal Belgian Society of Clinical Chemistry (RBSCC)
  9. Point
  10. Quantity quotient reporting. A proposal for a standardized presentation of laboratory results
  11. Counterpoint
  12. Quantity quotient reporting. Counterpoint
  13. Perspectives
  14. Increasing laboratory medicine activities in China: research and publications
  15. Contributions in Biochemistry and Molecular Biology from China and other top-ranking countries: a 10-year survey of the literature
  16. Genetics and Molecular Diagnostics
  17. SOCS3 and IRS-1 gene expression differs between genotype 1 and genotype 2 hepatitis C virus-infected HepG2 cells
  18. Polymorphisms of the β1-adrenergic receptor gene are associated with essential hypertension in Chinese
  19. Egyptian glycogen storage disease type III – identification of six novel AGL mutations, including a large 1.5 kb deletion and a missense mutation p.L620P with subtype IIId
  20. Novel rare alleles of ABCA1 are exclusively associated with extreme high-density lipoprotein-cholesterol levels among the Han Chinese
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  24. HbA1c measurements from dried blood spots: validation and patient satisfaction
  25. Pre-transplant serum concentrations of anti-endothelial cell antibody in panel reactive antibody negative renal recipients and its impact on acute rejection
  26. Cancer Diagnostics
  27. Detection of Meningeosis neoplastica by real-time quantitation of telomerase activity
  28. The prognostic utility of haptoglobin genotypes in squamous cell carcinoma of the head and neck
  29. Evaluation of a new method for the diagnosis of alterations of Lens culinaris agglutinin binding of thyroglobulin molecules in thyroid carcinoma
  30. The soluble transferrin receptor (TfR)-F-Index is not applicable as a test for iron status in patients with chronic lymphocytic leukemia
  31. Impact of calibration fitting models on the clinical value of chromogranin A
  32. Letters to the Editor
  33. Clinical value of a competitive NT-proBNP enzyme immunoassay compared to the Roche NT-proBNP platform
  34. Should gender-related reference values be used for total bilirubin?
  35. A novel nonsense mutation in the albumin gene (c.1275 C>A) causing analbuminemia in a Tunisian boy
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  37. 41st National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, 2nd Joint National Event SIBioC-SIMeL
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