Article
Licensed
Unlicensed Requires Authentication

Detection of Meningeosis neoplastica by real-time quantitation of telomerase activity

  • , , , , , , , , , , , , , and
Published/Copyright: September 11, 2009
Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 47 Issue 10

Abstract

Background: Analysis of cerebrospinal fluid (CSF) to discriminate between benign and malignant conditions is of fundamental importance for the physician and the patient because of the differential therapeutic options and resulting morbidity and mortality. Most human tumours demonstrate increased telomerase activity (TA). Recent technical advances in the detection of TA allow for sensitive and specific detection within 4 h. Thus, the detection of TA is suitable for routine clinical testing.

Methods: This study examines TA in cellular proteins in CSF from 111 patients compared to cytomorphological and laboratory examination.

Results: A positive result for TA in cellular proteins of CSF was correlated significantly with Meningeosis neoplastica, but not with non-malignant conditions. Telomerase was not detected in CSF supernatant, despite positive results in cellular proteins from identical patients. Furthermore, a 48-h time delay during the pre-analytic processing is not critical for detection of TA detection in native CSF when stored at room temperature.

Conclusions: We conclude that TA is a promising marker for the detection of Meningeosis neoplastica and warrants further study.

Clin Chem Lab Med 2009;47:1271–6.

Keywords: cerebrospinal fluid; Meningeosis neoplastica; real-time quantitative telomere repeat amplification protocol; telomerase activity
Corresponding author: Dr. Thomas Widmann, Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Homburg, 66421 Homburg/Saar, Germany Phone: +49-6841-1623944, Fax: +49-6841-1623965,
Received: 2009-4-4
Accepted: 2009-6-22
Published Online: 2009-09-11
Published in Print: 2009-10-01

©2009 by Walter de Gruyter Berlin New York

Articles in the same Issue

  1. Editorial
  2. Cardiac troponins: what we knew, what we know – where are we now?
  3. Review
  4. Measurement of circulating concentrations of cardiac troponin I and T in healthy subjects: a tool for monitoring myocardial tissue renewal?
  5. Opinion Papers
  6. A critical appraisal of experimental factors influencing the definition of the 99th percentile limit for cardiac troponins
  7. Genetic and biochemical heterogeneity of cardiac troponins: clinical and laboratory implications
  8. The future of hospital laboratories. Position statement from the Royal Belgian Society of Clinical Chemistry (RBSCC)
  9. Point
  10. Quantity quotient reporting. A proposal for a standardized presentation of laboratory results
  11. Counterpoint
  12. Quantity quotient reporting. Counterpoint
  13. Perspectives
  14. Increasing laboratory medicine activities in China: research and publications
  15. Contributions in Biochemistry and Molecular Biology from China and other top-ranking countries: a 10-year survey of the literature
  16. Genetics and Molecular Diagnostics
  17. SOCS3 and IRS-1 gene expression differs between genotype 1 and genotype 2 hepatitis C virus-infected HepG2 cells
  18. Polymorphisms of the β1-adrenergic receptor gene are associated with essential hypertension in Chinese
  19. Egyptian glycogen storage disease type III – identification of six novel AGL mutations, including a large 1.5 kb deletion and a missense mutation p.L620P with subtype IIId
  20. Novel rare alleles of ABCA1 are exclusively associated with extreme high-density lipoprotein-cholesterol levels among the Han Chinese
  21. –308G>A and –1031T>C tumor necrosis factor gene polymorphisms in Tunisian patients with coronary artery disease
  22. General Clinical Chemistry and Laboratory Medicine
  23. Error tracking in a clinical biochemistry laboratory
  24. HbA1c measurements from dried blood spots: validation and patient satisfaction
  25. Pre-transplant serum concentrations of anti-endothelial cell antibody in panel reactive antibody negative renal recipients and its impact on acute rejection
  26. Cancer Diagnostics
  27. Detection of Meningeosis neoplastica by real-time quantitation of telomerase activity
  28. The prognostic utility of haptoglobin genotypes in squamous cell carcinoma of the head and neck
  29. Evaluation of a new method for the diagnosis of alterations of Lens culinaris agglutinin binding of thyroglobulin molecules in thyroid carcinoma
  30. The soluble transferrin receptor (TfR)-F-Index is not applicable as a test for iron status in patients with chronic lymphocytic leukemia
  31. Impact of calibration fitting models on the clinical value of chromogranin A
  32. Letters to the Editor
  33. Clinical value of a competitive NT-proBNP enzyme immunoassay compared to the Roche NT-proBNP platform
  34. Should gender-related reference values be used for total bilirubin?
  35. A novel nonsense mutation in the albumin gene (c.1275 C>A) causing analbuminemia in a Tunisian boy
  36. Abstracts
  37. 41st National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, 2nd Joint National Event SIBioC-SIMeL
https://doi.org/10.1515/CCLM.2009.258
Keywords for this article
cerebrospinal fluid; Meningeosis neoplastica; real-time quantitative telomere repeat amplification protocol; telomerase activity

Articles in the same Issue

  1. Editorial
  2. Cardiac troponins: what we knew, what we know – where are we now?
  3. Review
  4. Measurement of circulating concentrations of cardiac troponin I and T in healthy subjects: a tool for monitoring myocardial tissue renewal?
  5. Opinion Papers
  6. A critical appraisal of experimental factors influencing the definition of the 99th percentile limit for cardiac troponins
  7. Genetic and biochemical heterogeneity of cardiac troponins: clinical and laboratory implications
  8. The future of hospital laboratories. Position statement from the Royal Belgian Society of Clinical Chemistry (RBSCC)
  9. Point
  10. Quantity quotient reporting. A proposal for a standardized presentation of laboratory results
  11. Counterpoint
  12. Quantity quotient reporting. Counterpoint
  13. Perspectives
  14. Increasing laboratory medicine activities in China: research and publications
  15. Contributions in Biochemistry and Molecular Biology from China and other top-ranking countries: a 10-year survey of the literature
  16. Genetics and Molecular Diagnostics
  17. SOCS3 and IRS-1 gene expression differs between genotype 1 and genotype 2 hepatitis C virus-infected HepG2 cells
  18. Polymorphisms of the β1-adrenergic receptor gene are associated with essential hypertension in Chinese
  19. Egyptian glycogen storage disease type III – identification of six novel AGL mutations, including a large 1.5 kb deletion and a missense mutation p.L620P with subtype IIId
  20. Novel rare alleles of ABCA1 are exclusively associated with extreme high-density lipoprotein-cholesterol levels among the Han Chinese
  21. –308G>A and –1031T>C tumor necrosis factor gene polymorphisms in Tunisian patients with coronary artery disease
  22. General Clinical Chemistry and Laboratory Medicine
  23. Error tracking in a clinical biochemistry laboratory
  24. HbA1c measurements from dried blood spots: validation and patient satisfaction
  25. Pre-transplant serum concentrations of anti-endothelial cell antibody in panel reactive antibody negative renal recipients and its impact on acute rejection
  26. Cancer Diagnostics
  27. Detection of Meningeosis neoplastica by real-time quantitation of telomerase activity
  28. The prognostic utility of haptoglobin genotypes in squamous cell carcinoma of the head and neck
  29. Evaluation of a new method for the diagnosis of alterations of Lens culinaris agglutinin binding of thyroglobulin molecules in thyroid carcinoma
  30. The soluble transferrin receptor (TfR)-F-Index is not applicable as a test for iron status in patients with chronic lymphocytic leukemia
  31. Impact of calibration fitting models on the clinical value of chromogranin A
  32. Letters to the Editor
  33. Clinical value of a competitive NT-proBNP enzyme immunoassay compared to the Roche NT-proBNP platform
  34. Should gender-related reference values be used for total bilirubin?
  35. A novel nonsense mutation in the albumin gene (c.1275 C>A) causing analbuminemia in a Tunisian boy
  36. Abstracts
  37. 41st National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, 2nd Joint National Event SIBioC-SIMeL
Downloaded on 12.4.2026 from https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2009.258/html
Scroll to top button