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Level of selenoprotein transcripts in peripheral leukocytes of patients with bladder cancer and healthy individuals
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Edyta Reszka
Published/Copyright:
September 1, 2009
Abstract
Background: Low concentrations of selenium (Se) in humans have been associated with risk of cancer. Selenoprotein mRNAs can potentially be regulated by Se status.
Methods: Se status, GPx1 Pro198Leu and Sep15 1125G/A genetic polymorphism and human (h)GPx1, hGPx3, hSep15 and hSeP1 transcript levels in peripheral leukocytes of 33 males with bladder cancer and 47 healthy male controls were analysed.
Results: All the subjects expressed detectable selenoprotein mRNA concentrations in leukocytes. Significantly lower expression of hGPx1, hGPx3, hSep15 and hSeP1 in leukocytes of bladder cancer patients compared to controls was observed. hGPx1, hGPx3 and hSep15 expression was significantly lower in non-smokers in the control group compared with smokers in the control group. A positive relationship between expression of all studied genes was also observed in non-smoking controls. Expression of hGPx3 and hSep15 gradually increased with tumour grade in patients with cancer. We did not find any association between selenoprotein mRNA levels, Se status and selenoprotein genetic polymorphism.
Conclusions: This study showed significant down-regulation of hGPx1, hGPx3, hSep15 and hSeP1 mRNA levels in leukocytes of patients with bladder cancer compared to controls. Selenoprotein transcript levels in circulating leukocytes of patients with bladder cancer and controls revealed no potential impact of Se status on selenoprotein expression.
Clin Chem Lab Med 2009;47:1125–32.
Received: 2009-3-31
Accepted: 2009-6-26
Published Online: 2009-09-01
Published in Print: 2009-09-01
©2009 by Walter de Gruyter Berlin New York
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Keywords for this article
bladder cancer;
genetic polymorphism;
leukocytes;
real-time PCR;
selenoproteins
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- Review
- Glomerular filtration rate measurement and prediction equations
- Genetics and Molecular Diagnostics
- Leptin gene (TTTC)n microsatellite polymorphism in pre-eclampsia and HELLP syndrome
- TaqIB polymorphism in cholesterol ester transfer protein (CETP) gene predicts future cardiovascular death in patients experiencing an acute coronary syndrome
- Peroxisome proliferator-activated receptor-γ (PPARγ) Pro12Ala polymorphism and risk for pediatric obesity
- Application of pyrosequencing to the identification of sequence variations in the cystic fibrosis transmembrane conductance regulator gene
- General Clinical Chemistry and Laboratory Medicine
- Relationship of serum bilirubin concentrations to kidney function and albuminuria in the United States adult population. Findings from the National Health and Nutrition Examination Survey 2001–2006
- Efficiency of test report delivery to the requesting physician in an outpatient setting: an observational study
- A case-control study on the relationship between HDL2b and non-alcoholic fatty liver disease in Chinese type 2 diabetic patients
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