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Quantitative telomerase activity in circulating human leukocytes: utility of real-time telomeric repeats amplification protocol (RQ-TRAP) in a clinical/epidemiological setting
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Vasco Lança
Veröffentlicht/Copyright:
4. Juni 2009
Abstract
Background: There is accumulating evidence from the epidemiological field of telomere biology that telomere length plays an important role in the pathophysiology of cardiovascular disease. The RNA-dependent DNA polymerase, telomerase, is essential in regulating telomere length by acting as a reverse transcriptase. However, the relationship between telomerase activity and telomere length in cardiovascular disease is unclear. This is due, in part, to the paucity of information on the utility of a quantitative and routine assay for the determination of telomerase activity in circulating blood leukocytes.
Methods: We used a validated, high-sensitive real-time quantitative telomeric repeat amplification protocol (RQ-TRAP) to determine telomerase activity in circulating blood leukocytes.
Results: The present investigation demonstrated direct and reliable detection of telomerase activity of circulating blood leukocytes.
Conclusion: The present investigation suggests the feasibility of using RQ-TRAP assay in routine screening of telomerase activity in blood specimens typically collected in a clinical/epidemiological setting.
Clin Chem Lab Med 2009;47:870–3.
Keywords: circulating leukocytes; real-time quantitative telomeric repeat amplification protocol (RQ-TRAP); telomerase activity
Received: 2009-2-6
Accepted: 2009-4-6
Published Online: 2009-06-04
Published in Print: 2009-07-01
©2009 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
circulating leukocytes;
real-time quantitative telomeric repeat amplification protocol (RQ-TRAP);
telomerase activity
Artikel in diesem Heft
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- Review
- The prevalence and risk factors for gallstone disease
- Opinion Paper
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- Genetics and Molecular Diagnostics
- Expression of RhoA and RhoC in colorectal carcinoma and its relations with clinicopathological parameters
- High-sensitive microarray substrates specifically designed to improve sensitivity for the identification of fetal paternally inherited sequences in maternal plasma
- A new CYP21A2 nonsense mutation causing severe 21-hydroxylase deficiency
- The haptoglobin phenotype is associated with the Epstein-Barr virus antibody titer
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