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Measurement uncertainty of test kit results – the ELISA example
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Miloslav Suchanek
Veröffentlicht/Copyright:
20. Mai 2009
Abstract
The measurement uncertainty budget for a clinical test-kit – an ELISA technique based on experimental data provided in the instruction manual – is presented. The proper characterization of the calibrators, within-plate homogeneity and the trueness of ELISA test-kits are identified as being major contributors to the combined uncertainty.
Clin Chem Lab Med 2009;47:808–10.
Received: 2009-2-4
Accepted: 2009-3-28
Published Online: 2009-05-20
Published in Print: 2009-07-01
©2009 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
calibrators;
combined uncertainty;
GUM;
In Vitro Diagnostics (IVD);
test-kit
Artikel in diesem Heft
- Editorials
- Change through continuity
- EFCC and Labs are Vital announce an Award for Outcomes Research in Laboratory Medicine
- Review
- The prevalence and risk factors for gallstone disease
- Opinion Paper
- Measurement uncertainty of test kit results – the ELISA example
- Genetics and Molecular Diagnostics
- Expression of RhoA and RhoC in colorectal carcinoma and its relations with clinicopathological parameters
- High-sensitive microarray substrates specifically designed to improve sensitivity for the identification of fetal paternally inherited sequences in maternal plasma
- A new CYP21A2 nonsense mutation causing severe 21-hydroxylase deficiency
- The haptoglobin phenotype is associated with the Epstein-Barr virus antibody titer
- General Clinical Chemistry and Laboratory Medicine
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- Cerebrospinal fluid and serum uric acid levels in patients with multiple sclerosis
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