Multidimensional protein identification technology for clinical proteomic analysis
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Pierluigi Mauri
Abstract
Proteomics technologies demonstrate enormous data-gathering capabilities to discover disease-specific biomarkers in serum, plasma, urine, tissue and other biological samples. The traditional way to achieve this consists of protein separation performed using two-dimensional polyacrylamide gel electrophoresis (2DE). However, the 2DE approach has its drawbacks with respect to automation, sensitivity, and throughput. Considerable efforts have been devoted to the development of non-gel-based proteome separation technologies able to resolve complex protein and peptide mixtures prior to mass spectrometric (MS) analysis. This review discusses some of the most recent advances in multidimensional peptide separation techniques compatible with MS analysis, including gel electrophoresis, isoelectric focusing, capillary electrophoresis and liquid chromatography techniques. Based on future perspectives and our experiences, special attention is given to the application of two-dimensional chromatographic separation coupled to MS, the so-called multidimensional protein identification technology approach.
Clin Chem Lab Med 2009;47:636–46.
©2009 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Editorial
- A focus on recent advances in proteomics – one step closer to entrance into the clinical arena
- Reviews
- Proteomics in protein misfolding diseases
- Multidimensional protein identification technology for clinical proteomic analysis
- Technical advances in proteomics mass spectrometry: identification of post-translational modifications
- Mass spectrometry based proteomics profiling as diagnostic tool in oncology: current status and future perspective
- Research Papers
- Thrombin induces broad spectrum proteolysis in human serum samples
- Influence of sample storage duration on serum protein profiles assessed by surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF MS)
- Proteomics of exhaled breath: methodological nuances and pitfalls
- Pancreatic cancer biomarkers discovery by surface-enhanced laser desorption and ionization time-of-flight mass spectrometry
- Approaching clinical proteomics: current state and future fields of application in fluid proteomics
- Genetics and Molecular Diagnostics
- Detection of Hpdel in healthy individuals and cancer patients in Taiwan
- Increased CA 19-9 level in patients without malignant disease
- General Clinical Chemistry and Laboratory Medicine
- Quantitation of cerebrospinal fluid lactic acid in infectious and non-infectious neurological diseases
- Proficiency testing project for brain natriuretic peptide (BNP) and the N-terminal part of the propeptide of BNP (NT-proBNP) immunoassays: the CardioOrmocheck study
- Reference Values and Biological Variations
- Influence of tourniquet application on venous blood sampling for serum chemistry, hematological parameters, leukocyte activation and erythrocyte mechanical properties
- Systemic cytokine response following exercise-induced muscle damage in humans
- Letters to the Editor
- CACNA1A gene non-synonymous single nucleotide polymorphisms and common migraine in Italy: a case-control association study with a micro-array technology
- Proficiency test of plasma free and total metanephrines: report from a study group
