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Technical advances in proteomics mass spectrometry: identification of post-translational modifications
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Angela Amoresano
Published/Copyright:
May 11, 2009
Abstract
The importance of post-translational modifications (PTMs) of proteins has become evident in the proteomic era as it plays a critical role in modulating cellular function, and can vary in response to different stimuli thereby tuning cellular mechanisms. Assessment of PTMs on a proteomic scale is a challenging task since they are substoichiometric, transient and reversible. Moreover, the amount of post-translationally modified proteins is generally very small when compared to their unmodified counterparts. Existing methodologies for identification of PTMs essentially relies on enrichment procedure to selectively increase the amount of modified peptides. These procedures need to be integrated with sophisticated mass spectrometric methods to enable the identifications of PTMs. Although the strategies developed so far are not optimal, a number of examples will be given where the combination of innovative separation methods along with advanced mass spectrometric analyses provide positive results. These experiences are leading the way for the next generation of proteomic approaches for identification of a wide range of PTMs.
Clin Chem Lab Med 2009;47:647–65.
Keywords: glycosylation; nitration; phosphorylation; post-translational modifications (PTMs); proteomics; tandem mass spectrometry (MS/MS)
Received: 2008-10-11
Accepted: 2009-3-23
Published Online: 2009-05-11
Published in Print: 2009-06-01
©2009 by Walter de Gruyter Berlin New York
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Keywords for this article
glycosylation;
nitration;
phosphorylation;
post-translational modifications (PTMs);
proteomics;
tandem mass spectrometry (MS/MS)
Articles in the same Issue
- Editorial
- A focus on recent advances in proteomics – one step closer to entrance into the clinical arena
- Reviews
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- Mass spectrometry based proteomics profiling as diagnostic tool in oncology: current status and future perspective
- Research Papers
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- Influence of sample storage duration on serum protein profiles assessed by surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF MS)
- Proteomics of exhaled breath: methodological nuances and pitfalls
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