Article
Licensed
Unlicensed
Requires Authentication
Pre-analytical variation of some haematological quantities
-
Mariona García-Panyella
Published/Copyright:
June 20, 2008
Abstract
Background: It is customary to assume that pre-analytical variation is negligible if all pre-analytical sources of variation have been standardised. The aim of this study was to quantify the pre-analytical variation of some haematological quantities frequently measured in clinical laboratories.
Methods: The experimental design considers different sources of pre-analytical variation that usually occur day-to-day for samples from patients.
Results: The results demonstrate that for concentrations of erythrocytes, haemoglobin, leukocytes, neutrophilocytes (segmented) and lymphocytes, and for the volume fraction of erythrocytes (“packed cell volume”) in blood, the pre-analytical variance is not negligible.
Conclusions: We suggest that these variances should be taken into account when estimating the uncertainty of measurement for patient results.
Clin Chem Lab Med 2008;46:1168–70.
Received: 2008-1-5
Accepted: 2008-4-10
Published Online: 2008-06-20
Published in Print: 2008-08-01
©2008 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Review
- Insulin-like growth factor-I and complications of prematurity: a focus on bronchopulmonary dysplasia
- Minireview
- Highly sensitive thyroglobulin measurements in differentiated thyroid carcinoma management
- Genetics and Molecular Diagnostics
- Proteomic pattern of plasma in off-pump coronary artery bypass surgery
- Association of CHRNA2 polymorphisms with overweight/obesity and clinical characteristics in a Korean population
- Relevance of glutathione S-transferase M1 and cytochrome P450 1A1 genetic polymorphisms to the development of head and neck cancers
- Association of the insertion/deletion gene polymorphism of the apolipoprotein B signal peptide with myocardial infarction in Tunisian patients
- The influence of smoking and homocysteine on subclinical atherosclerosis is modified by the connexin37 C1019T polymorphism – The Cardiovascular Risk in Young Finns Study
- Tests for the measurement of factor VII-activating protease (FSAP) activity and antigen levels in citrated plasma, their correlation to PCR testing, and utility for the detection of the Marburg I-polymorphism of FSAP
- An improved quality control for bisulfite-PCR-based DNA methylation analysis: cycle threshold value
- General Clinical Chemistry and Laboratory Medicine
- Can E-cadherin and CD34 be used as indicators of prognosis for hepatocellular carcinoma patients?
- Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems
- Calcium oxalate stone formation risk – a case of disturbed relative concentrations of urinary components
- Half-lives of albumin and cholinesterase in critically ill patients
- Early detection of an early onset infection in the neonate based on measurements of procalcitonin and C-reactive protein concentrations in cord blood
- Serum neopterin, nitric oxide, inducible nitric oxide synthase and tumor necrosis factor-α levels in patients with ischemic heart disease
- Estimation of serum M-protein concentration from polyclonal immunoglobulins: an alternative to serum protein electrophoresis and standard immunochemical procedures
- Are serum lipids measured on stroke admission prognostic?
- Reference Values and Biological Variations
- Pre-analytical variation of some haematological quantities
- Long-term stability of endogenous B-type natriuretic peptide after storage at –20°C or –80°C
- Can both EDTA and citrate plasma samples be used in measurements of fibrinogen and C-reactive protein concentrations?
- Non-homogeneous separation of triglycerides, γ-glutamyltransferase, C-reactive protein and lactate dehydrogenase after centrifugation of lithium-heparin tubes
- Validation and Outcome Studies
- Computer-assisted detection of monoclonal components: results from the multicenter study for the evaluation of CASPER (Computer Assisted Serum Protein Electrophoresis Recognizer) algorithm
- Quality indicators and specifications for strategic and support processes in laboratory medicine
- Evaluation of the DCA Vantage analyzer for HbA1c assay
- Letters to the Editor
- Optimization of imprecision and cost saving on hemoglobin A1c controls through aliquot freezing
- Estimation of glomerular filtration rate by the modification of diet in renal disease (MDRD) equation in patients with sickle cell disease
- Abstracts
- Swiss MedLab 2008: International Congress, September 16–19, 2008, Montreux, Switzerland
- 4th Biologie Prospective Santorini Conference, September 21–23, 2008, Santorini, Greece
Keywords for this article
pre-analytical variation;
pre-metrological variation;
uncertainty of measurement
Articles in the same Issue
- Review
- Insulin-like growth factor-I and complications of prematurity: a focus on bronchopulmonary dysplasia
- Minireview
- Highly sensitive thyroglobulin measurements in differentiated thyroid carcinoma management
- Genetics and Molecular Diagnostics
- Proteomic pattern of plasma in off-pump coronary artery bypass surgery
- Association of CHRNA2 polymorphisms with overweight/obesity and clinical characteristics in a Korean population
- Relevance of glutathione S-transferase M1 and cytochrome P450 1A1 genetic polymorphisms to the development of head and neck cancers
- Association of the insertion/deletion gene polymorphism of the apolipoprotein B signal peptide with myocardial infarction in Tunisian patients
- The influence of smoking and homocysteine on subclinical atherosclerosis is modified by the connexin37 C1019T polymorphism – The Cardiovascular Risk in Young Finns Study
- Tests for the measurement of factor VII-activating protease (FSAP) activity and antigen levels in citrated plasma, their correlation to PCR testing, and utility for the detection of the Marburg I-polymorphism of FSAP
- An improved quality control for bisulfite-PCR-based DNA methylation analysis: cycle threshold value
- General Clinical Chemistry and Laboratory Medicine
- Can E-cadherin and CD34 be used as indicators of prognosis for hepatocellular carcinoma patients?
- Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems
- Calcium oxalate stone formation risk – a case of disturbed relative concentrations of urinary components
- Half-lives of albumin and cholinesterase in critically ill patients
- Early detection of an early onset infection in the neonate based on measurements of procalcitonin and C-reactive protein concentrations in cord blood
- Serum neopterin, nitric oxide, inducible nitric oxide synthase and tumor necrosis factor-α levels in patients with ischemic heart disease
- Estimation of serum M-protein concentration from polyclonal immunoglobulins: an alternative to serum protein electrophoresis and standard immunochemical procedures
- Are serum lipids measured on stroke admission prognostic?
- Reference Values and Biological Variations
- Pre-analytical variation of some haematological quantities
- Long-term stability of endogenous B-type natriuretic peptide after storage at –20°C or –80°C
- Can both EDTA and citrate plasma samples be used in measurements of fibrinogen and C-reactive protein concentrations?
- Non-homogeneous separation of triglycerides, γ-glutamyltransferase, C-reactive protein and lactate dehydrogenase after centrifugation of lithium-heparin tubes
- Validation and Outcome Studies
- Computer-assisted detection of monoclonal components: results from the multicenter study for the evaluation of CASPER (Computer Assisted Serum Protein Electrophoresis Recognizer) algorithm
- Quality indicators and specifications for strategic and support processes in laboratory medicine
- Evaluation of the DCA Vantage analyzer for HbA1c assay
- Letters to the Editor
- Optimization of imprecision and cost saving on hemoglobin A1c controls through aliquot freezing
- Estimation of glomerular filtration rate by the modification of diet in renal disease (MDRD) equation in patients with sickle cell disease
- Abstracts
- Swiss MedLab 2008: International Congress, September 16–19, 2008, Montreux, Switzerland
- 4th Biologie Prospective Santorini Conference, September 21–23, 2008, Santorini, Greece
