Home Long-term stability of endogenous B-type natriuretic peptide after storage at –20°C or –80°C
Article
Licensed
Unlicensed Requires Authentication

Long-term stability of endogenous B-type natriuretic peptide after storage at –20°C or –80°C

  • Marta Pereira , Ana Azevedo , Milton Severo and Henrique Barros
Published/Copyright: June 27, 2008
Become an author with De Gruyter Brill
Submit Manuscript Author Information
Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 46 Issue 8

Abstract

Background: B-type natriuretic peptide (BNP) is a biomarker used in the investigation of heart failure and other cardiovascular diseases. Our aim was to analyze the degradation of BNP in plasma samples kept at –20°C or at –80°C after 3 months or 1 year of storage.

Methods: Using a Biosite assay-Triage BNP test, we evaluated 20 subjects with plasma BNP concentrations ranging from normal to high. Plasma aliquots were separated in five eppendorfs, one for immediate measurement after centrifugation, two for storage at –20°C and two at –80°C. We measured BNP after 3 months and 1 year. The significance level was set at p<0.05 (one-tailed test).

Results: There was a significant decrease of 1% per month in BNP concentration. However, there was a significant interaction between time and group (high vs. low BNP). Low BNP values did not change over time, while high BNP decreased by 2% per month. The decrease over time did not depend on the storage temperature.

Conclusions: The storage of the samples at –20°C or at –80°C did not prevent BNP degradation, especially for high BNP values. For low BNP values, we did not observe a significant decay at either 3 months or 1 year. These results are valid for the Biosite assay-Triage BNP test.

Clin Chem Lab Med 2008;46:1171–4.

Keywords: B-type natriuretic peptide; immunoassay; storage; temperature
Corresponding author: Marta Pereira, Department of Hygiene and Epidemiology, University of Porto Medical School, Alameda Prof Hernâni Monteiro, 4200-319 Porto, Portugal Phone: +351-22-551-3652, Fax: +351-22-551-3653,
Received: 2008-2-14
Accepted: 2008-4-8
Published Online: 2008-06-27
Published in Print: 2008-08-01

©2008 by Walter de Gruyter Berlin New York

Articles in the same Issue

  1. Review
  2. Insulin-like growth factor-I and complications of prematurity: a focus on bronchopulmonary dysplasia
  3. Minireview
  4. Highly sensitive thyroglobulin measurements in differentiated thyroid carcinoma management
  5. Genetics and Molecular Diagnostics
  6. Proteomic pattern of plasma in off-pump coronary artery bypass surgery
  7. Association of CHRNA2 polymorphisms with overweight/obesity and clinical characteristics in a Korean population
  8. Relevance of glutathione S-transferase M1 and cytochrome P450 1A1 genetic polymorphisms to the development of head and neck cancers
  9. Association of the insertion/deletion gene polymorphism of the apolipoprotein B signal peptide with myocardial infarction in Tunisian patients
  10. The influence of smoking and homocysteine on subclinical atherosclerosis is modified by the connexin37 C1019T polymorphism – The Cardiovascular Risk in Young Finns Study
  11. Tests for the measurement of factor VII-activating protease (FSAP) activity and antigen levels in citrated plasma, their correlation to PCR testing, and utility for the detection of the Marburg I-polymorphism of FSAP
  12. An improved quality control for bisulfite-PCR-based DNA methylation analysis: cycle threshold value
  13. General Clinical Chemistry and Laboratory Medicine
  14. Can E-cadherin and CD34 be used as indicators of prognosis for hepatocellular carcinoma patients?
  15. Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems
  16. Calcium oxalate stone formation risk – a case of disturbed relative concentrations of urinary components
  17. Half-lives of albumin and cholinesterase in critically ill patients
  18. Early detection of an early onset infection in the neonate based on measurements of procalcitonin and C-reactive protein concentrations in cord blood
  19. Serum neopterin, nitric oxide, inducible nitric oxide synthase and tumor necrosis factor-α levels in patients with ischemic heart disease
  20. Estimation of serum M-protein concentration from polyclonal immunoglobulins: an alternative to serum protein electrophoresis and standard immunochemical procedures
  21. Are serum lipids measured on stroke admission prognostic?
  22. Reference Values and Biological Variations
  23. Pre-analytical variation of some haematological quantities
  24. Long-term stability of endogenous B-type natriuretic peptide after storage at –20°C or –80°C
  25. Can both EDTA and citrate plasma samples be used in measurements of fibrinogen and C-reactive protein concentrations?
  26. Non-homogeneous separation of triglycerides, γ-glutamyltransferase, C-reactive protein and lactate dehydrogenase after centrifugation of lithium-heparin tubes
  27. Validation and Outcome Studies
  28. Computer-assisted detection of monoclonal components: results from the multicenter study for the evaluation of CASPER (Computer Assisted Serum Protein Electrophoresis Recognizer) algorithm
  29. Quality indicators and specifications for strategic and support processes in laboratory medicine
  30. Evaluation of the DCA Vantage analyzer for HbA1c assay
  31. Letters to the Editor
  32. Optimization of imprecision and cost saving on hemoglobin A1c controls through aliquot freezing
  33. Estimation of glomerular filtration rate by the modification of diet in renal disease (MDRD) equation in patients with sickle cell disease
  34. Abstracts
  35. Swiss MedLab 2008: International Congress, September 16–19, 2008, Montreux, Switzerland
  36. 4th Biologie Prospective Santorini Conference, September 21–23, 2008, Santorini, Greece
https://doi.org/10.1515/CCLM.2008.223
Keywords for this article
B-type natriuretic peptide; immunoassay; storage; temperature

Articles in the same Issue

  1. Review
  2. Insulin-like growth factor-I and complications of prematurity: a focus on bronchopulmonary dysplasia
  3. Minireview
  4. Highly sensitive thyroglobulin measurements in differentiated thyroid carcinoma management
  5. Genetics and Molecular Diagnostics
  6. Proteomic pattern of plasma in off-pump coronary artery bypass surgery
  7. Association of CHRNA2 polymorphisms with overweight/obesity and clinical characteristics in a Korean population
  8. Relevance of glutathione S-transferase M1 and cytochrome P450 1A1 genetic polymorphisms to the development of head and neck cancers
  9. Association of the insertion/deletion gene polymorphism of the apolipoprotein B signal peptide with myocardial infarction in Tunisian patients
  10. The influence of smoking and homocysteine on subclinical atherosclerosis is modified by the connexin37 C1019T polymorphism – The Cardiovascular Risk in Young Finns Study
  11. Tests for the measurement of factor VII-activating protease (FSAP) activity and antigen levels in citrated plasma, their correlation to PCR testing, and utility for the detection of the Marburg I-polymorphism of FSAP
  12. An improved quality control for bisulfite-PCR-based DNA methylation analysis: cycle threshold value
  13. General Clinical Chemistry and Laboratory Medicine
  14. Can E-cadherin and CD34 be used as indicators of prognosis for hepatocellular carcinoma patients?
  15. Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems
  16. Calcium oxalate stone formation risk – a case of disturbed relative concentrations of urinary components
  17. Half-lives of albumin and cholinesterase in critically ill patients
  18. Early detection of an early onset infection in the neonate based on measurements of procalcitonin and C-reactive protein concentrations in cord blood
  19. Serum neopterin, nitric oxide, inducible nitric oxide synthase and tumor necrosis factor-α levels in patients with ischemic heart disease
  20. Estimation of serum M-protein concentration from polyclonal immunoglobulins: an alternative to serum protein electrophoresis and standard immunochemical procedures
  21. Are serum lipids measured on stroke admission prognostic?
  22. Reference Values and Biological Variations
  23. Pre-analytical variation of some haematological quantities
  24. Long-term stability of endogenous B-type natriuretic peptide after storage at –20°C or –80°C
  25. Can both EDTA and citrate plasma samples be used in measurements of fibrinogen and C-reactive protein concentrations?
  26. Non-homogeneous separation of triglycerides, γ-glutamyltransferase, C-reactive protein and lactate dehydrogenase after centrifugation of lithium-heparin tubes
  27. Validation and Outcome Studies
  28. Computer-assisted detection of monoclonal components: results from the multicenter study for the evaluation of CASPER (Computer Assisted Serum Protein Electrophoresis Recognizer) algorithm
  29. Quality indicators and specifications for strategic and support processes in laboratory medicine
  30. Evaluation of the DCA Vantage analyzer for HbA1c assay
  31. Letters to the Editor
  32. Optimization of imprecision and cost saving on hemoglobin A1c controls through aliquot freezing
  33. Estimation of glomerular filtration rate by the modification of diet in renal disease (MDRD) equation in patients with sickle cell disease
  34. Abstracts
  35. Swiss MedLab 2008: International Congress, September 16–19, 2008, Montreux, Switzerland
  36. 4th Biologie Prospective Santorini Conference, September 21–23, 2008, Santorini, Greece
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 7.9.2025 from https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2008.223/html
Scroll to top button