Artikel
Lizenziert
Nicht lizenziert
Erfordert eine Authentifizierung
Use of SELDI-TOF mass spectrometry for identification of new biomarkers: potential and limitations
Veröffentlicht/Copyright:
1. November 2007
Abstract
Surface-enhanced laser desorption time of flight mass spectrometry (SELDI-TOF-MS) is an important proteomic technology that is immediately available for the high throughput analysis of complex protein samples. Over the last few years, several studies have demonstrated that comparative protein profiling using SELDI-TOF-MS breaks new ground in diagnostic protein analysis particularly with regard to the identification of novel biomarkers. Importantly, researchers have acquired a better understanding also of the limitations of this technology and various pitfalls in biomarker discovery. Bearing these in mind, great emphasis must be placed on the development of rigorous standards and quality control procedures for the pre-analytical as well as the analytical phase and subsequent bioinformatics applied to analysis of the data. To avoid the risk of false-significant results studies must be designed carefully and control groups accurately selected. In addition, appropriate tools, already established for analysis of highly complex microarray data, need to be applied to protein profiling data. To validate the significance of any candidate biomarker derived from pilot studies in appropriately designed prospective multi-center studies is mandatory; reproducibility of the clinical results must be shown over time and in different diagnostic settings. SELDI-TOF-MS-based studies that are in compliance with these requirements are now required; only a few have been published so far. In the meantime, further evaluation and optimization of both technique and marker validation strategies are called for before MS-based proteomic algorithms can be translated into routine laboratory testing.
Clin Chem Lab Med 2007;45:1435–49.
Keywords: biomarker identification; mass spectrometry; protein profiling; proteomics; surface-enhanced laser desorption time of flight mass spectrometry (SELDI-TOF-MS)
Published Online: 2007-11-01
Published in Print: 2007-11-01
©2007 by Walter de Gruyter Berlin New York
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Artikel in diesem Heft
- Current state and future directions of neurochemical biomarkers for Alzheimer's disease
- Use of SELDI-TOF mass spectrometry for identification of new biomarkers: potential and limitations
- Analysis of ferritin genes in Parkinson disease
- Multimers and adiponectin gene 276G>T polymorphism in the Japanese population residing in rural areas
- Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp.
- A polymorphism in the resistin gene promoter is related to increased C-reactive protein levels in patients with coronary artery disease
- Association of a 27-bp repeat polymorphism in intron 4 of endothelial constitutive nitric oxide synthase gene with myocardial infarction in Tunisian patients
- How useful are housekeeping genes? Variable expression in melanoma metastases
- Combined androgen blockade therapy can convert RT-PCR detection of prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSMA) transcripts from positive to negative in the peripheral blood of patients with clinically localized prostate cancer and increase biochemical failure-free survival after curative therapy
- Isobutyryl-CoA dehydrogenase deficiency with a novel ACAD8 gene mutation detected by tandem mass spectrometry newborn screening
- Circulating levels of osteoprotegerin and receptor activator of NF-κB ligand in patients with chronic renal failure
- Altered angiogenesis in preeclampsia: evaluation of a new test system for measuring placental growth factor
- Impact of rosiglitazone on the expression of β3-AR in the stable cell lines expressed β3-AR gene
- Biochemical and hormonal composition, cytological examination of thyroid cyst fluid, and comparison according to gender and color of cyst fluid
- Redefining functional sensitivity of thyroglobulin assay on Immulite platform: implications in thyroid cancer management
- Developmental changes in the L-arginine/nitric oxide pathway from infancy to adulthood: plasma asymmetric dimethylarginine levels decrease with age
- Umbilical cord blood serum procalcitonin by Time-Resolved Amplified Cryptate Emission (TRACE) technology: reference values of a potential marker of vertically transmitted neonatal sepsis
- Point-of-care creatinine testing in children at risk for sudden deterioration of renal function
- Utility of PFA-100® closure time vs. optical aggregometry in assessing the efficacy of platelet membrane glycoprotein IIb/IIIa antagonists in vitro
- Reliability assessment of glucose measurement by HemoCue analyser in a neonatal intensive care unit
- Validation study of Axis-Shield carbohydrate-deficient transferrin (CDT) on ARCHITECT® chemistry analyzer
- High free serum acrolein levels in bacterial infection and other disease states associated with oxidative stress: a potential biomarker?
- Increased serum S100B protein in chronic schizophrenic patients in Korea
- Addendum to the recent report of EC4 on the accreditation of medical laboratories in the European Union
- Reply to the letter written by Haeckel
- The systemic inflammatory response syndrome C-reactive protein and transthyretin conundrum
- Transthyretin, nutrition, and inflammation: response to Dr. Bernstein
- Erratum
Schlagwörter für diesen Artikel
biomarker identification;
mass spectrometry;
protein profiling;
proteomics;
surface-enhanced laser desorption time of flight mass spectrometry (SELDI-TOF-MS)
Artikel in diesem Heft
- Current state and future directions of neurochemical biomarkers for Alzheimer's disease
- Use of SELDI-TOF mass spectrometry for identification of new biomarkers: potential and limitations
- Analysis of ferritin genes in Parkinson disease
- Multimers and adiponectin gene 276G>T polymorphism in the Japanese population residing in rural areas
- Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp.
- A polymorphism in the resistin gene promoter is related to increased C-reactive protein levels in patients with coronary artery disease
- Association of a 27-bp repeat polymorphism in intron 4 of endothelial constitutive nitric oxide synthase gene with myocardial infarction in Tunisian patients
- How useful are housekeeping genes? Variable expression in melanoma metastases
- Combined androgen blockade therapy can convert RT-PCR detection of prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSMA) transcripts from positive to negative in the peripheral blood of patients with clinically localized prostate cancer and increase biochemical failure-free survival after curative therapy
- Isobutyryl-CoA dehydrogenase deficiency with a novel ACAD8 gene mutation detected by tandem mass spectrometry newborn screening
- Circulating levels of osteoprotegerin and receptor activator of NF-κB ligand in patients with chronic renal failure
- Altered angiogenesis in preeclampsia: evaluation of a new test system for measuring placental growth factor
- Impact of rosiglitazone on the expression of β3-AR in the stable cell lines expressed β3-AR gene
- Biochemical and hormonal composition, cytological examination of thyroid cyst fluid, and comparison according to gender and color of cyst fluid
- Redefining functional sensitivity of thyroglobulin assay on Immulite platform: implications in thyroid cancer management
- Developmental changes in the L-arginine/nitric oxide pathway from infancy to adulthood: plasma asymmetric dimethylarginine levels decrease with age
- Umbilical cord blood serum procalcitonin by Time-Resolved Amplified Cryptate Emission (TRACE) technology: reference values of a potential marker of vertically transmitted neonatal sepsis
- Point-of-care creatinine testing in children at risk for sudden deterioration of renal function
- Utility of PFA-100® closure time vs. optical aggregometry in assessing the efficacy of platelet membrane glycoprotein IIb/IIIa antagonists in vitro
- Reliability assessment of glucose measurement by HemoCue analyser in a neonatal intensive care unit
- Validation study of Axis-Shield carbohydrate-deficient transferrin (CDT) on ARCHITECT® chemistry analyzer
- High free serum acrolein levels in bacterial infection and other disease states associated with oxidative stress: a potential biomarker?
- Increased serum S100B protein in chronic schizophrenic patients in Korea
- Addendum to the recent report of EC4 on the accreditation of medical laboratories in the European Union
- Reply to the letter written by Haeckel
- The systemic inflammatory response syndrome C-reactive protein and transthyretin conundrum
- Transthyretin, nutrition, and inflammation: response to Dr. Bernstein
- Erratum
