Article
Licensed
Unlicensed
Requires Authentication
Methylprednisolone, cortisol and the cell-mediated immune response in children after ventricular septal defect repair
-
Stefan Grosek
Published/Copyright:
October 10, 2007
Abstract
Background: This study evaluated the effects of methylprednisolone on cortisol and cell-mediated immune response (T-lymphocytes and HLA-DR+ monocytes) in peripheral blood after open-heart surgery with cardiopulmonary bypass (CPB) for ventricular septal defect.
Methods: A prospective observational study was carried out in a tertiary multidisciplinary neonatal and paediatric intensive care unit. Ten children under 2 years of age received methylprednisolone succinate (30 mg/kg body weight) in CPB priming solutions before the CPB system was connected to the patient during surgery. Before and immediately after and at 24 and 96 h after the operation, T-lymphocytes and HLA-DR+ monocytes were measured by flow cytometry, and methylprednisolone, methylprednisolone succinate and cortisol in blood plasma were assayed by liquid chromatography-mass spectrometry.
Results: The children were divided into groups with normal cardiac index (CI) and low CI. No significant differences in methylprednisolone and cortisol concentrations before and after surgery were found between the two groups. The normal CI group exhibited more than a three-fold decrease in T-lymphocytes 24 h after surgery and a two-fold decrease in HLA-DR+ monocyte fluorescence immediately after surgery.
Conclusions: Children with normal and low CI were differentiated by T-lymphocytes and HLA-DR+ monocytes. Since no differences in methylprednisolone exposure and cortisol plasma levels between the low-CI and normal-CI groups were found, it can be concluded that factors other than methylprednisolone must contribute to differences in the cell-mediated response.
Clin Chem Lab Med 2007;45:1366–72.
Keywords: cell-mediated immune response; children; methylprednisolone; NONMEM software; open-heart surgery
Received: 2007-3-14
Accepted: 2007-5-24
Published Online: 2007-10-10
Published in Print: 2007-10-01
©2007 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Atrial natriuretic peptide and related peptides
- The −374A allele of the receptor for advanced glycation end products (RAGE) gene promoter is a protective factor against cardiovascular lesions in type 2 diabetes mellitus patients
- Biochemical properties of endogenous presenilin 1 and presenilin 2 in cultured human B-lymphocytes
- CD36 polymorphism and its relationship with body mass index and coronary artery disease in a Korean population
- Preservation of RNA for functional analysis of separated alleles in yeast: comparison of snap-frozen and RNALater® solid tissue storage methods
- Mother-child double incompatibility at vWA and D5S818 loci in paternity testing
- Matrix metalloprotease-2 and -9 concentration and activity in serum and culture medium samples: a methodological reappraisal
- Are changes in blood-ethanol concentration during storage analytically significant? Importance of method imprecision
- Measurement of arginine derivatives in pediatric patients with chronic kidney disease using high-performance liquid chromatography-tandem mass spectrometry
- The soluble transferrin receptor reflects tumor load in chronic lymphocytic leukemia
- Calculated low-density lipoprotein cholesterol remains a viable and important test for screening and targeting therapy
- Angiotensin-converting enzyme (ACE) I/D corrected serum ACE activity and severity assessment of community-acquired pneumonia
- Differentiating transudative from exudative pleural effusion: should we measure effusion cholesterol dehydrogenase?
- Association of classical and related inflammatory markers with high-sensitivity C-reactive protein in healthy individuals: results from the Stanislas cohort
- Cytokines and growth factors in mostly atherosclerotic patients on hemodialysis determined by biochip array technology
- Use of a solid-phase extraction with radioimmunoassay to identify the proportional bias of clinical B-type natriuretic peptide immunoassay: the impact of plasma matrix and antibody multispecificity
- High plasma levels of tissue inhibitor of metalloproteinase-1 (TIMP-1) and interleukin-8 (IL-8) characterize patients prone to ventricular fibrillation complicating myocardial infarction
- Methylprednisolone, cortisol and the cell-mediated immune response in children after ventricular septal defect repair
- Standardization of γ-glutamyltransferase assays by intermethod calibration. Effect on determining common reference limits
- The effect of endurance exercise-induced lactacidosis on biochemical markers of bone turnover
- Serum biobank certification and the establishment of quality controls for biological fluids: examples of serum biomarker stability after temperature variation
- Diagnostic usefulness of a third-generation anti-cyclic citrulline antibody test in patients with recent-onset polyarthritis
- Clinical significance of the laboratory determination of low serum copper in adults
- National survey on critical values reporting in a cohort of Italian laboratories
- Influence of haemodialysis on the NT-proBNP plasma concentration
- C–588T polymorphism of the human glutamate-cysteine ligase modifier subunit gene is not associated with the risk and extent of ischemic heart disease in a German cohort
- Has homocysteine shrunk?
Keywords for this article
cell-mediated immune response;
children;
methylprednisolone;
NONMEM software;
open-heart surgery
Articles in the same Issue
- Atrial natriuretic peptide and related peptides
- The −374A allele of the receptor for advanced glycation end products (RAGE) gene promoter is a protective factor against cardiovascular lesions in type 2 diabetes mellitus patients
- Biochemical properties of endogenous presenilin 1 and presenilin 2 in cultured human B-lymphocytes
- CD36 polymorphism and its relationship with body mass index and coronary artery disease in a Korean population
- Preservation of RNA for functional analysis of separated alleles in yeast: comparison of snap-frozen and RNALater® solid tissue storage methods
- Mother-child double incompatibility at vWA and D5S818 loci in paternity testing
- Matrix metalloprotease-2 and -9 concentration and activity in serum and culture medium samples: a methodological reappraisal
- Are changes in blood-ethanol concentration during storage analytically significant? Importance of method imprecision
- Measurement of arginine derivatives in pediatric patients with chronic kidney disease using high-performance liquid chromatography-tandem mass spectrometry
- The soluble transferrin receptor reflects tumor load in chronic lymphocytic leukemia
- Calculated low-density lipoprotein cholesterol remains a viable and important test for screening and targeting therapy
- Angiotensin-converting enzyme (ACE) I/D corrected serum ACE activity and severity assessment of community-acquired pneumonia
- Differentiating transudative from exudative pleural effusion: should we measure effusion cholesterol dehydrogenase?
- Association of classical and related inflammatory markers with high-sensitivity C-reactive protein in healthy individuals: results from the Stanislas cohort
- Cytokines and growth factors in mostly atherosclerotic patients on hemodialysis determined by biochip array technology
- Use of a solid-phase extraction with radioimmunoassay to identify the proportional bias of clinical B-type natriuretic peptide immunoassay: the impact of plasma matrix and antibody multispecificity
- High plasma levels of tissue inhibitor of metalloproteinase-1 (TIMP-1) and interleukin-8 (IL-8) characterize patients prone to ventricular fibrillation complicating myocardial infarction
- Methylprednisolone, cortisol and the cell-mediated immune response in children after ventricular septal defect repair
- Standardization of γ-glutamyltransferase assays by intermethod calibration. Effect on determining common reference limits
- The effect of endurance exercise-induced lactacidosis on biochemical markers of bone turnover
- Serum biobank certification and the establishment of quality controls for biological fluids: examples of serum biomarker stability after temperature variation
- Diagnostic usefulness of a third-generation anti-cyclic citrulline antibody test in patients with recent-onset polyarthritis
- Clinical significance of the laboratory determination of low serum copper in adults
- National survey on critical values reporting in a cohort of Italian laboratories
- Influence of haemodialysis on the NT-proBNP plasma concentration
- C–588T polymorphism of the human glutamate-cysteine ligase modifier subunit gene is not associated with the risk and extent of ischemic heart disease in a German cohort
- Has homocysteine shrunk?
