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Use of a solid-phase extraction with radioimmunoassay to identify the proportional bias of clinical B-type natriuretic peptide immunoassay: the impact of plasma matrix and antibody multispecificity
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George O.A. Naik
Published/Copyright:
October 10, 2007
Abstract
Background: Circulating immunoreactive B-type natriuretic peptide-32 (ir-BNP-32) has diagnostic and prognostic values in heart failure. We compared, in parallel, a point-of-care (POC) test (Triage® BNP Test) of whole plasma and radioimmunoassay (RIA) of solid-phase extracted (SPE) plasma (SPE/RIA) utilizing a novel copolymer column, in the measurement of patient ir-BNP-32 concentrations.
Methods: Approximately 0.25 mL thawed plasma was transferred to a BNP test device and inserted in a Triage® Meter Plus, which gave ir-BNP-32 concentration in pg/mL. Concurrently, for the SPE/RIA measurement, 1.0 mL plasma was acidified and extracted with an OASIS® column; eluate dried, reconstituted and quantified by RIA.
Results: Inter-day coefficient of variation for both methods were <15%. Plasma SPE recovery was 75.2%. POC correlated with recovery corrected SPE/RIA for ir-BNP-32, r=0.843 (p<0.0001) and the Passing-Bablok model was POC ir-BNP-32=1.43× (recovery corrected SPE/RIA ir-BNP-32)+9.75 ng/L (n=81). A proportional bias was also evident from the Bland-Altman plot, r=0.716 (p<0.0001).
Conclusions: A proportional bias is responsible for plasma ir-BNP-32 concentration differences between whole plasma POC test and recovery corrected SPE/RIA measurements. Ir-BNP-32 assays are influenced by plasma matrix and antibody multispecificity. Consequently, consistent analytical accuracy between immunoassays is necessary to attain a single ir-BNP-32 concentration threshold for diagnosis.
Clin Chem Lab Med 2007;45:1353–9.
Keywords: antibody multispecificity; heart failure; plasma immunoreactive BNP-32; point-of-care test; radioimmunoassay; solid-phase extraction
Received: 2007-1-18
Accepted: 2007-6-1
Published Online: 2007-10-10
Published in Print: 2007-10-01
©2007 by Walter de Gruyter Berlin New York
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Keywords for this article
antibody multispecificity;
heart failure;
plasma immunoreactive BNP-32;
point-of-care test;
radioimmunoassay;
solid-phase extraction
Articles in the same Issue
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- CD36 polymorphism and its relationship with body mass index and coronary artery disease in a Korean population
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