Article
Licensed
Unlicensed
Requires Authentication
On detecting interference from endogenous antibodies in immunoassays by doubling dilutions test
-
Adel A.A. Ismail
Published/Copyright:
July 8, 2007
Abstract
The clinical laboratory may perform additional test or tests to ascertain the integrity of immunoassay analyses when the results are in doubt. Doubling serial dilutions is one of these confirmatory tests. Doubling dilutions is a useful and informative test for detecting inaccuracy and potential interference from endogenous antibodies in immunoassays. Despite its technical simplicity, the science underpinning this test is highly complex. Appreciating such complexities could help in understanding the various outcomes of such tests from misleadingly inaccurate but virtually linear/parallel results, despite the presence of interfering antibodies in some immunoassays, to an array of non-linearity/non-parallelism synonymous with interference in others. A lack of parallelism/linearity could identify some 60% of samples with inaccurate and potentially misleading results caused by endogenous interfering antibodies. Assessing real-time binding kinetics using techniques such as non-linear regression analysis or linearised transformations such as the Scatchard plot could help in identifying more samples in which the doubling dilutions test remains linear and parallel.
Clin Chem Lab Med 2007;45:851–4.
Received: 2007-2-15
Accepted: 2007-3-22
Published Online: 2007-07-08
Published in Print: 2007-07-01
©2007 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Pharmacogenomics and Personalized Medicine – a global reality check
- Pharmacogenomics and adverse drug reactions in diagnostic and clinical practice
- Impact of genetic polymorphisms on the risk of lipid disorders in patients on anti-HIV therapy
- Polymorphisms in genes involved in DNA repair and metabolism of xenobiotics in individual susceptibility to sporadic diffuse gastric cancer
- Effect of 13-cis-retinoic acid on the genetic expression profile of human umbilical vein endothelial cells (HUVECs) determined by microarray
- Association between T102C and A–1438G polymorphisms in the serotonin receptor 2A (5-HT2A) gene and schizophrenia: relevance for treatment with antipsychotic drugs
- A1166C polymorphism of the angiotensin AT1 receptor (AT1R) gene alters endothelial response to statin treatment
- Third Santorini conference pharmacogenomics workshop report: “Pharmacogenomics at the crossroads: what else than good science will be needed for the field to become part of Personalized Medicine?”
- On detecting interference from endogenous antibodies in immunoassays by doubling dilutions test
- High-sensitivity C-reactive protein concentration in patients with myocardial infarction-environmental factors, and polymorphisms in interleukin-10 and CD14 genes
- Tankyrase-1 mRNA expression in bladder cancer and paired urine sediment: preliminary experience
- A novel application of melting curves: utility of peak area calculation for relative methylation quantification
- Paraoxonase 1 gene polymorphisms in angiographically assessed coronary artery disease: evidence for gender interaction among Brazilians
- Tumor necrosis factor-α and oxidant status are essential participating factors in unexplained recurrent spontaneous abortions
- Paraoxonase and platelet-activating factor acetylhydrolase activities in lipoproteins of β-thalassemia/hemoglobin E patients
- Analysis of glomerular filtration rate, serum cystatin C levels, and renal resistive index values in cirrhosis patients
- Direct determination of selenium in human serum by inductively coupled plasma-collision cell-mass spectrometry
- Within-subject variation of plasma resistin levels over a 1-year period
- Global DNA methylation measured by liquid chromatography-tandem mass spectrometry: analytical technique, reference values and determinants in healthy subjects
- Values for free/total prostate-specific antigen ratio as a function of age: necessity of reference validation in a Turkish population
- Comparison of the Roche COBAS Amplicor™ Monitor, Roche COBAS Ampliprep™/COBAS Taqman™ and Abbott RealTime™ Test assays for quantification of hepatitis C virus and HIV RNA
- Genetic cystic fibrosis transmembrane regulator 4016insT D1152H compound heterozygosity and male infertility: an Italian case report
- Reference intervals for serum cystatin C in healthy Mexican adults
- Could standard ISO 15189 be useful for the conundrum of CA 19-9/GI Monitor measurement?
- Quality control strategy to verify day-to-day imprecision of length of blood sedimentation reaction measurements with the TEST-1 analyzer
- Applicability of α-fetoprotein-concanavalin A (AFP-ConA) binding to discriminate between germinal or hepatic origin of AFP in germ cell tumour patients during chemotherapy or follow-up
- Proposal of a candidate international conventional reference measurement procedure for free thyroxine in serum
- Balkan Clinical Laboratory Federation − past, present and future trends
Articles in the same Issue
- Pharmacogenomics and Personalized Medicine – a global reality check
- Pharmacogenomics and adverse drug reactions in diagnostic and clinical practice
- Impact of genetic polymorphisms on the risk of lipid disorders in patients on anti-HIV therapy
- Polymorphisms in genes involved in DNA repair and metabolism of xenobiotics in individual susceptibility to sporadic diffuse gastric cancer
- Effect of 13-cis-retinoic acid on the genetic expression profile of human umbilical vein endothelial cells (HUVECs) determined by microarray
- Association between T102C and A–1438G polymorphisms in the serotonin receptor 2A (5-HT2A) gene and schizophrenia: relevance for treatment with antipsychotic drugs
- A1166C polymorphism of the angiotensin AT1 receptor (AT1R) gene alters endothelial response to statin treatment
- Third Santorini conference pharmacogenomics workshop report: “Pharmacogenomics at the crossroads: what else than good science will be needed for the field to become part of Personalized Medicine?”
- On detecting interference from endogenous antibodies in immunoassays by doubling dilutions test
- High-sensitivity C-reactive protein concentration in patients with myocardial infarction-environmental factors, and polymorphisms in interleukin-10 and CD14 genes
- Tankyrase-1 mRNA expression in bladder cancer and paired urine sediment: preliminary experience
- A novel application of melting curves: utility of peak area calculation for relative methylation quantification
- Paraoxonase 1 gene polymorphisms in angiographically assessed coronary artery disease: evidence for gender interaction among Brazilians
- Tumor necrosis factor-α and oxidant status are essential participating factors in unexplained recurrent spontaneous abortions
- Paraoxonase and platelet-activating factor acetylhydrolase activities in lipoproteins of β-thalassemia/hemoglobin E patients
- Analysis of glomerular filtration rate, serum cystatin C levels, and renal resistive index values in cirrhosis patients
- Direct determination of selenium in human serum by inductively coupled plasma-collision cell-mass spectrometry
- Within-subject variation of plasma resistin levels over a 1-year period
- Global DNA methylation measured by liquid chromatography-tandem mass spectrometry: analytical technique, reference values and determinants in healthy subjects
- Values for free/total prostate-specific antigen ratio as a function of age: necessity of reference validation in a Turkish population
- Comparison of the Roche COBAS Amplicor™ Monitor, Roche COBAS Ampliprep™/COBAS Taqman™ and Abbott RealTime™ Test assays for quantification of hepatitis C virus and HIV RNA
- Genetic cystic fibrosis transmembrane regulator 4016insT D1152H compound heterozygosity and male infertility: an Italian case report
- Reference intervals for serum cystatin C in healthy Mexican adults
- Could standard ISO 15189 be useful for the conundrum of CA 19-9/GI Monitor measurement?
- Quality control strategy to verify day-to-day imprecision of length of blood sedimentation reaction measurements with the TEST-1 analyzer
- Applicability of α-fetoprotein-concanavalin A (AFP-ConA) binding to discriminate between germinal or hepatic origin of AFP in germ cell tumour patients during chemotherapy or follow-up
- Proposal of a candidate international conventional reference measurement procedure for free thyroxine in serum
- Balkan Clinical Laboratory Federation − past, present and future trends
