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Paraoxonase and platelet-activating factor acetylhydrolase activities in lipoproteins of β-thalassemia/hemoglobin E patients
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Noppawan Phumala Morales
Published/Copyright:
July 8, 2007
Abstract
Background: Iron-induced oxidative stress may be implicated in the alteration of the lipoprotein-associated antioxidant enzymes paraoxonase 1 (PON1) and platelet-activating factor acetylhydrolase (PAF-AH), leading to atherosclerosis-related vascular complication in patients with β-thalassemia hemoglobin E (β-thal/Hb E).
Methods: Plasma and lipoprotein enzyme activities of PON1 and PAF-AH were studied in 13 mild to moderate and 15 severe cases of β-thal/Hb E in comparison with 15 normal subjects.
Results: PON1 activity was significantly reduced in association with oxidative stress in the patients. There were significant correlations between high-density lipoprotein (HDL)-PON1 activity and oxidative stress markers, including plasma levels of α-tocopherol (r=0.694 p<0.001) and the ratio of cholesteryl linoleate to cholesteryl oleate (CL/CO, r=0.662, p<0.001) in HDL. On the other hand, PAF-AH activity was markedly increased in patients by approximately two-fold and three- to four-fold in plasma and lipoproteins, respectively. Significant correlations of low-density lipoprotein (LDL) and HDL-PAF-AH activity with plasma iron, α-tocopherol and the CL/CO ratio were also demonstrated.
Conclusions: We suggest that impairment of PON1 activity may be directly caused by oxidative damage, while increased PAF-AH activity possibly results from oxidative stress-induced inflammatory response in β-thal/Hb E patients.
Clin Chem Lab Med 2007;45:884–9.
Keywords: lipoprotein; oxidative stress; paraoxonase; platelet-activating factor acetylhydrolase; β-thalassemia
Received: 2006-12-13
Accepted: 2007-2-25
Published Online: 2007-07-08
Published in Print: 2007-07-01
©2007 by Walter de Gruyter Berlin New York
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Keywords for this article
lipoprotein;
oxidative stress;
paraoxonase;
platelet-activating factor acetylhydrolase;
β-thalassemia
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- Effect of 13-cis-retinoic acid on the genetic expression profile of human umbilical vein endothelial cells (HUVECs) determined by microarray
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