Evaluation of cardiovascular risk factors and related clinical markers in healthy young Japanese adults
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Chiaki Yagura
Abstract
Background: Since atherosclerosis is a slowly progressive process at a young age, effective preventive measures should be taken early in life to prevent future events associated with cardiovascular disease.
Methods: The study population comprised 132 young Japanese adults (mean age 21.4 years, range 18–31 years). We screened plasma total homocysteine and serum folate levels and evaluated mean carotid intima-media thickness and cardio-ankle vascular index.
Results: Multiple regression analysis after adjustment for age and sex revealed that only folate levels were significantly correlated with plasma total homocysteine levels (β=–0.37, p=0.028). Carotid intima-media thickness adjusted for age and sex and compared between quintiles of total homocysteine levels was significantly increased in the highest quintile compared with other quintiles. Cardio-ankle vascular index increased with age in both women and men, but no additional determinants were identified in young adults.
Conclusion: Serum folate is an independent determinant of plasma total homocysteine levels, and mild hyperhomocysteinemia may represent a risk factor for increased carotid intima-media thickness, even in young adults. Comprehensive health education from the early period of life, including the suggestion of appropriate dietary measures, is important for effective prevention of future atherosclerosis.
Clin Chem Lab Med 2007;45:220–5.
References
1. Oren A, Vos LE, Uiterwaal CS, Grobbee DE, Bots ML. Cardiovascular risk factors and increased carotid intima-media thickness in healthy young adults: the Atherosclerosis Risk in Young Adults (ARYA) Study. Arch Intern Med2003;163:1787–92.10.1001/archinte.163.15.1787Suche in Google Scholar PubMed
2. Ridker PM, Stampfer MJ, Rifai N. Novel risk factors for systemic atherosclerosis: a comparison of C-reactive protein, fibrinogen, homocysteine, lipoprotein(a), and standard cholesterol screening as predictors of peripheral arterial disease. J Am Med Assoc2001;285:2481–5.10.1001/jama.285.19.2481Suche in Google Scholar PubMed
3. de Bree A, Verschuren WM, Kromhout D, Kluijtmans LA, Blom HJ. Homocysteine determinants and the evidence to what extent homocysteine determines the risk of coronary heart disease. Pharmacol Rev2002;54:599–618.10.1124/pr.54.4.599Suche in Google Scholar PubMed
4. Voutilainen S, Morrow JD, Roberts LJ II, Alfthan G, Alho H, Nyyssonen K, et al. Enhanced in vivo lipid peroxidation at elevated plasma total homocysteine levels. Arterioscler Thromb Vasc Biol1999;19:1263–6.10.1161/01.ATV.19.5.1263Suche in Google Scholar
5. Klerk M, Verhoef P, Clarke R, Blom HJ, Kok FJ, Schouten EG; MTHFR Studies Collaboration Group. MTHFR 677C→T polymorphism and risk of coronary heart disease: a meta-analysis. J Am Med Assoc2002;288:2023–31.10.1001/jama.288.16.2023Suche in Google Scholar PubMed
6. Robinson K, Arheart K, Refsum H, Brattstrom L, Boers G, Ueland P, et al. Low circulating folate and vitamin B6 concentrations: risk factors for stroke, peripheral vascular disease, and coronary artery disease. European COMAC Group. Circulation1998;97:437–43.10.1161/01.CIR.97.5.437Suche in Google Scholar PubMed
7. Homocysteine Lowering Trialists' Collaboration. Lowering blood homocysteine with folic acid based supplements: meta-analysis of randomised trials. Br Med J1998;316:894–8.10.1136/bmj.316.7135.894Suche in Google Scholar
8. Wilmink HW, Stroes ES, Erkelens WD, Gerritsen WB, Wever R, Banga JD, et al. Influence of folic acid on postprandial endothelial dysfunction. Arterioscler Thromb Vasc Biol2000;20:185–8.10.1161/01.ATV.20.1.185Suche in Google Scholar PubMed
9. Chambless LE, Folsom AR, Clegg LX, Sharrett AR, Shahar E, Nieto FJ, et al. Carotid wall thickness is predictive of incident clinical stroke: the Atherosclerosis Risk in Communities (ARIC) study. Am J Epidemiol2000;151:478–87.10.1093/oxfordjournals.aje.a010233Suche in Google Scholar PubMed
10. Bots ML, Hoes AW, Koudstaal PJ, Hofman A, Grobbee DE. Common carotid intima-media thickness and risk of stroke and myocardial infarction: the Rotterdam Study. Circulation1997;96:1432–7.10.1161/01.CIR.96.5.1432Suche in Google Scholar
11. Raitakari OT, Adams MR, McCredie RJ, Griffiths KA, Celermajer DS. Arterial endothelial dysfunction related to passive smoking is potentially reversible in healthy young adults. Ann Intern Med1999;130:578–81.10.7326/0003-4819-130-7-199904060-00017Suche in Google Scholar
12. Sass C, Zannad F, Herbeth B, Salah D, Chapet O, Siest G, et al. Apolipoprotein E4, lipoprotein lipase C447 and angiotensin-I converting enzyme deletion alleles were not associated with increased wall thickness of carotid and femoral arteries in healthy subjects from the Stanislas cohort. Atherosclerosis1998;140:89–95.10.1016/S0021-9150(98)00120-8Suche in Google Scholar
13. Davis PH, Dawson JD, Mahoney LT, Lauer RM. Increased carotid intimal-medial thickness and coronary calcification are related in young and middle-aged adults. The Muscatine study. Circulation1999;100:838–42.10.1161/01.CIR.100.8.838Suche in Google Scholar
14. Homma S, Hirose N, Ishida H, Ishii T, Araki G. Carotid plaque and intima-media thickness assessed by B-mode ultrasonography in subjects ranging from young adults to centenarians. Stroke2001;32:830–5.10.1161/01.STR.32.4.830Suche in Google Scholar
15. Adachi H, Hirai Y, Fujiura Y, Matsuoka H, Satoh A, Imaizumi T. Plasma homocysteine levels and atherosclerosis in Japan: epidemiological study by use of carotid ultrasonography. Stroke2002;33:2177–81.10.1161/01.STR.0000026861.18199.89Suche in Google Scholar
16. van Popele NM, Grobbee DE, Bots ML, Asmar R, Topouchian J, Reneman RS, et al. Association between arterial stiffness and atherosclerosis: the Rotterdam Study. Stroke2001;32:454–60.10.1161/01.STR.32.2.454Suche in Google Scholar
17. Zureik M, Bureau JM, Temmar M, Adamopoulos C, Courbon D, Bean K, et al. Echogenic carotid plaques are associated with aortic arterial stiffness in subjects with subclinical carotid atherosclerosis. Hypertension2003;41:519–27.10.1161/01.HYP.0000054978.86286.92Suche in Google Scholar
18. Sutton-Tyrrell K, Wildman R, Newman A, Kuller LH. Extent of cardiovascular risk reduction associated with treatment of isolated systolic hypertension. Arch Intern Med2003;163:2728–31.10.1001/archinte.163.22.2728Suche in Google Scholar
19. Woodside JV, McMahon R, Gallagher AM, Cran GW, Boreham CA, Murray LJ, et al. Total homocysteine is not a determinant of arterial pulse wave velocity in young healthy adults. Atherosclerosis2004;177:337–44.10.1016/j.atherosclerosis.2004.07.001Suche in Google Scholar
20. Yambe T, Yoshizawa M, Saijo Y, Yamaguchi T, Shibata M, Konno S, et al. Brachio-ankle pulse wave velocity and cardio-ankle vascular index (CAVI). Biomed Pharmacother2004;58:S95–8.10.1016/S0753-3322(04)80015-5Suche in Google Scholar
21. Shirai K, Utino J, Otsuka K, Takata M. A novel blood pressure-independent arterial wall stiffness parameter; cardio-ankle vascular index (CAVI). J Atheroscler Thromb2006;13:101–7.10.5551/jat.13.101Suche in Google Scholar
22. Yambe T, Meng X, Hou X, Wang Q, Sekine K, Shiraishi Y, et al. Cardio-ankle vascular index (CAVI) for the monitoring of the atherosclerosis after heart transplantation. Biomed Pharmacother2005;59:S177–9.10.1016/S0753-3322(05)80028-9Suche in Google Scholar
23. Garcia AJ, Apitz-Castro R. Plasma total homocysteine quantification: an improvement of the classical high-performance liquid chromatographic method with fluorescence detection of the thiol-SBD derivatives. J Chromatogr B2002;779:359–63.10.1016/S1570-0232(02)00401-4Suche in Google Scholar
24. Hara T, Takamura N, Akashi S, Nakazato M, Maeda T, Wada M, et al. Evaluation of clinical markers of atherosclerosis in young and elderly Japanese adults. Clin Chem Lab Med2006;44:824–9.10.1515/CCLM.2006.149Suche in Google Scholar
25. Lussier-Cacan S, Xhignesse M, Piolot A, Selhub J, Davignon J, Genest J Jr. Plasma total homocysteine in healthy subjects: sex-specific relation with biological traits. Am J Clin Nutr1996;64:587–93.10.1093/ajcn/64.4.587Suche in Google Scholar
26. de Bree A, Verschuren WM, Blom HJ, de Graaf-Hess A, Trijbels FJ, Kromhout D. The homocysteine distribution: (mis)judging the burden. J Clin Epidemiol2001;54:462–9.10.1016/S0895-4356(00)00341-3Suche in Google Scholar
27. Ueland PM, Refsum H, Stabler SP, Malinow MR, Andersson A, Allen RH. Total homocysteine in plasma or serum: methods and clinical applications. Clin Chem1993;39:1764–79.10.1093/clinchem/39.9.1764Suche in Google Scholar
28. Selhub J, Jacques PF, Bostom AG, D'Agostino RB, Wilson PW, Belanger AJ, et al. Association between plasma homocysteine concentrations and extracranial carotid-artery stenosis. N Engl J Med1995;332:286–91.10.1056/NEJM199502023320502Suche in Google Scholar PubMed
29. Spence JD, Malinow MR, Barnett PA, Marian AJ, Freeman D, Hegele RA. Plasma homocyst(e)ine concentration, but not MTHFR genotype, is associated with variation in carotid plaque area. Stroke1999;30:969–73.10.1161/01.STR.30.5.969Suche in Google Scholar PubMed
30. McQuillan BM, Beilby JP, Nidorf M, Thompson PL, Hung J. Hyperhomocysteinemia but not the C677T mutation of methylenetetrahydrofolate reductase is an independent risk determinant of carotid wall thickening. The Perth Carotid Ultrasound Disease Assessment Study (CUDAS) Circulation1999;99:2383–8.10.1161/01.CIR.99.18.2383Suche in Google Scholar
31. Durga J, Verhoef P, Bots ML, Schouten E. Homocysteine and carotid intima-media thickness: a critical appraisal of the evidence. Atherosclerosis2004;176:1–19.10.1016/j.atherosclerosis.2003.11.022Suche in Google Scholar PubMed
©2007 by Walter de Gruyter Berlin New York
Artikel in diesem Heft
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- Hyperhomocysteinemia and cardiovascular risk in postmenopausal women: the role of folate supplementation
- Association of vitamin B12, folate and homocysteine with functional and pathological characteristics of the elderly in a mountainous village in Sicily
- Association of thyroid dysfunction with vitamin B12, folate and plasma homocysteine levels in the elderly: a population-based study in Sicily
- Desmond (Des) Kenny MSc MCB FICI FRCPath Eur Clin Chem, 1941–2006
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- Novel molecular defect in the platelet ADP receptor P2Y12 of a patient with haemorrhagic diathesis
- Retrospective study of monoclonal gammopathies detected in the clinical laboratory of a Spanish healthcare district: 14-year series
- Renal impairment compromises the use of total homocysteine and methylmalonic acid but not total vitamin B12 and holotranscobalamin in screening for vitamin B12 deficiency in the aged
- Diagnostic value of calculated testosterone indices in the assessment of polycystic ovary syndrome
- Amniotic fibronectin fragmentation and expression of its domains, sialyl and fucosyl glycotopes associated with pregnancy complicated by intrauterine infection
- Predicted influence of sample hematocrit on injected mass of internal standard in mass spectrometry assays utilizing simple protein precipitation for sample preparation
- Evaluation of cardiovascular risk factors and related clinical markers in healthy young Japanese adults
- Cardiac natriuretic peptides after myocardial infarction: relationship with infarct size, left ventricular function and remodelling assessed by 99mTc-sestamibi gated-single photon emission tomography
- Plasma homocysteine cutoff values for venous thrombosis
- Lipid transfer protein activities in subjects with impaired glucose tolerance
- Heat shock protein 70-1 gene expression in pediatric heart surgery using blood cardioplegia
- Biochemical reference intervals for sex hormones with a new AutoDelfia method in aged men
- The Italian External Quality Control Programme for cystic fibrosis molecular diagnosis: 4 years of activity
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- To the Editor: Author reply; Nakano et al. Clin Chem Lab Med 2006;44(5):522–532
- Accreditation of medical laboratories in the European Union
- Prevalence-dependent decision limits for the early detection of type 2 diabetes mellitus in venous blood, venous plasma and capillary blood during glucose challenge
Artikel in diesem Heft
- Associations between folate, vitamin B12, homocysteine and pathologies related to aging: the need to consider complex nutrient-nutrient and gene-nutrient interactions and the functional and socio-economic determinants in population-based studies
- Hyperhomocysteinemia and cardiovascular risk in postmenopausal women: the role of folate supplementation
- Association of vitamin B12, folate and homocysteine with functional and pathological characteristics of the elderly in a mountainous village in Sicily
- Association of thyroid dysfunction with vitamin B12, folate and plasma homocysteine levels in the elderly: a population-based study in Sicily
- Desmond (Des) Kenny MSc MCB FICI FRCPath Eur Clin Chem, 1941–2006
- Antibodies to citrullinated peptides: a significant step forward in the early diagnosis of rheumatoid arthritis
- Patient hydration: a major source of laboratory uncertainty
- Determination of the hepatitis C virus subtype: comparison of sequencing and reverse hybridization assays
- Quantification of relative changes in specific mRNAs from frozen whole blood – methodological considerations and clinical implications
- HER-2 protein concentrations in breast cancer cells increase before immunohistochemical and fluorescence in situ hybridization analysis turn positive
- Distinct alleles of mannose-binding lectin (MBL) and surfactant proteins A (SP-A) in patients with chronic cavitary pulmonary aspergillosis and allergic bronchopulmonary aspergillosis
- Novel molecular defect in the platelet ADP receptor P2Y12 of a patient with haemorrhagic diathesis
- Retrospective study of monoclonal gammopathies detected in the clinical laboratory of a Spanish healthcare district: 14-year series
- Renal impairment compromises the use of total homocysteine and methylmalonic acid but not total vitamin B12 and holotranscobalamin in screening for vitamin B12 deficiency in the aged
- Diagnostic value of calculated testosterone indices in the assessment of polycystic ovary syndrome
- Amniotic fibronectin fragmentation and expression of its domains, sialyl and fucosyl glycotopes associated with pregnancy complicated by intrauterine infection
- Predicted influence of sample hematocrit on injected mass of internal standard in mass spectrometry assays utilizing simple protein precipitation for sample preparation
- Evaluation of cardiovascular risk factors and related clinical markers in healthy young Japanese adults
- Cardiac natriuretic peptides after myocardial infarction: relationship with infarct size, left ventricular function and remodelling assessed by 99mTc-sestamibi gated-single photon emission tomography
- Plasma homocysteine cutoff values for venous thrombosis
- Lipid transfer protein activities in subjects with impaired glucose tolerance
- Heat shock protein 70-1 gene expression in pediatric heart surgery using blood cardioplegia
- Biochemical reference intervals for sex hormones with a new AutoDelfia method in aged men
- The Italian External Quality Control Programme for cystic fibrosis molecular diagnosis: 4 years of activity
- Standardization of ischemia-modified albumin testing: adjustment for serum albumin
- Is simultaneous measurement of anti-thyroid peroxidase and anti-thyroglobulin antibodies clinically useful in patients with thyroid dysfunction?
- To the Editor: In reply to Nakano et al. Clin Chem Lab Med 2006;44(5):522–532
- To the Editor: Author reply; Nakano et al. Clin Chem Lab Med 2006;44(5):522–532
- Accreditation of medical laboratories in the European Union
- Prevalence-dependent decision limits for the early detection of type 2 diabetes mellitus in venous blood, venous plasma and capillary blood during glucose challenge
