Amniotic fibronectin fragmentation and expression of its domains, sialyl and fucosyl glycotopes associated with pregnancy complicated by intrauterine infection
-
Lidia Hirnle
Abstract
Background: The presence of fibronectin fragments has been observed in some inflammatory diseases and is believed to reflect tissue breakdown. In this study, possible fibronectin fragmentation and alterations in its domain and sialyl and fucosyl glycotope expressions were analyzed in amniotic fluids in relation to intrauterine infection.
Methods: Samples of amniotic fluid were taken from normal pregnancies and pregnancies (28 and 42 weeks) complicated by intrauterine infection. Fibronectin fragmentation was analyzed by immunoblotting. The expression of cellular, fibrin, C-terminal and EDA fibronectin domains, as well as α2,3- and α2,6-linked sialic acids, and α1,6-, α1,3- and α1,2-linked fucoses, was determined by ELISA, using domain-specific monoclonal antibodies and specific lectins, respectively.
Results: Amniotic fibronectin immunoblots from pregnancies with intrauterine infection revealed three groups of results. In group 1, with the native fibronectin band, and in group 2 with bands of native fibronectin and several fibronectin fragments, only higher α1,6-linked fucose expression was observed. In the infection group 3, characterized by profound fragmentation of fibronectin, lower expression of all fibronectin domains analyzed and of α1,6-linked sialic acid and α1,2-linked fucose was found.
Conclusions: Amniotic fibronectin status was found to be associated with pregnancy complicated by intrauterine infection. Such alterations could have a potential diagnostic value in the prevention of or intervention in fetal intrauterine infection.
Clin Chem Lab Med 2007;45:208–14.
References
1. Ffrench-Constant Ch. Alternative splicing of fibronectin - many different proteins but few different functions. Exp Cell Res1995;21:261–71.10.1006/excr.1995.1374Search in Google Scholar
2. Wierzbicka-Patynowski I, Schwarzbauer JE. The ins and outs of fibronectin matrix assembly. J Cell Sci2003;116:3269–76.10.1242/jcs.00670Search in Google Scholar
3. Ingham K. http://home.comcast.net/∼kennethingham/newsite/index.htm 2006.Search in Google Scholar
4. Yamada K. Fibronectin peptides in cell migration and wound repair. J Clin Invest2000;105:1507–9.10.1172/JCI10119Search in Google Scholar
5. Abe Y, Bui-Thanh NA, Ballantyne CM, Burns AR. Extra domain A and type III connecting segment of fibronectin in assembly and cleavage. Biochim Biophys Res Commun2005;338:1640–7.10.1016/j.bbrc.2005.10.134Search in Google Scholar
6. Cukierman E, Pankov R, Yamada KM. Cell interactions with three-dimensional matrices. Curr Opin Cell Biol2002;14:633–9.10.1016/S0955-0674(02)00364-2Search in Google Scholar
7. Hynes RO. The dynamic dialogue between wells and matrices: implications of fibronectin's elasticity. Proc Natl Acad Sci USA1999;96:2588–90.10.1073/pnas.96.6.2588Search in Google Scholar
8. Tajiri M, Yoshida S, Wada Y. Differential analysis of site-specific glycans on plasma and cellular fibronectins: application of a hydrophilic affinity method for glycopeptide enrichment. Glycobiology2005;15:1332–40.10.1093/glycob/cwj019Search in Google Scholar
9. Zhu BC, Fisher SF, Pande H, Calaycay J, Shively JE, Laine RA: Human placental (fetal) fibronectin: increased glycosylation and higher protease resistance than plasma fibronectin. Presence of polylactoamine glycopeptides and properties of a 44-kilodalton chymotryptic collagen-binding domain: difference from human plasma fibronectin. J Biol Chem1984;259:3962–70.10.1016/S0021-9258(17)43190-5Search in Google Scholar
10. Krusius T, Fukuda M, Dell A, Ruoslahti E. Structure of the carbohydrate units of human amniotic fluid fibronectin. J Biol Chem1985;260:4110–6.10.1016/S0021-9258(18)89238-9Search in Google Scholar
11. Hampel DJ, Köttgen B, Dudenhausen JW, Köttgen E. Fetal fibronectin as a marker for an imminent (preterm) delivery. A new technique using the glycoprotein lectin immunosorbent assay. J Immunol Methods1999;224:31–42.10.1016/S0022-1759(99)00006-XSearch in Google Scholar
12. Fukuda M, Levery SB, Hakomori S. Carbohydrate structure of hamster plasma fibronectin. Evidence for chemical diversity between cellular and plasma fibronectins. J Biol Chem1982;257:6856–60.10.1016/S0021-9258(18)34508-3Search in Google Scholar
13. Takamoto M, Endo T, Isemura M, Kochibe N, Kobata A. Structure of asparagine-linked oligosaccharides of human placental fibronectin. J Biochem1989;105:742–50.10.1093/oxfordjournals.jbchem.a122738Search in Google Scholar
14. Köttgen E, Hell B, Müller C, Kainer F, Tauber R. Developmental changes in the glycosylation and binding properties of human fibronectins. Characterization of glycan structures and ligand binding of human fibronectins from adult plasma, cord blood and amniotic fluid. Biol Chem Hoppe-Seyler1989;370:1285–94.10.1515/bchm3.1989.370.2.1285Search in Google Scholar
15. Yi M, Ruoslahti E. Fibronectin fragment inhibits tumor growth, angiogenesis, and metastasis. Proc Natl Acad Sci USA2001;98:620–4.10.1073/pnas.98.2.620Search in Google Scholar
16. Barilla ML, Carsons SE. Fibronectin fragments and their role in inflammatory arthritis; Semin Arthritis Rheum2000;29:252–65.10.1016/S0049-0172(00)80012-8Search in Google Scholar
17. Huynh QN, Wang S, Tafolla E, Gansky SA, Kapila S, Armitage GC, et al. Specific fibronectin fragments of periodontal disease status. J Peridontol2002;73:1101–10.10.1902/jop.2002.73.10.1101Search in Google Scholar
18. Magnusson MK, Mosher DF. Fibronectin, structure, assembly and cardiovascular implications. Arterioscler Thromb Vasc Biol1998;18:1363–70.10.1161/01.ATV.18.9.1363Search in Google Scholar
19. Kątnik-Prastowska I, Przybysz M, Chełmońska-Soyta A. Fibronectin fragments in human seminal plasma. Acta Biochem Polon2005;52:557–60.10.18388/abp.2005_3473Search in Google Scholar
20. Kątnik-Prastowska I, Kratz ME, Faundez R, Chełmońska-Soyta A. Lower expression of α2,3- sialylated fibronectin glycoform and appearance of asialo-fibronectin glycoform are associated with high concentrations of fibronectin in human seminal plasma with abnormal semen parameters. Clin Chem Lab Med2006;44:1119–25.10.1515/CCLM.2006.193Search in Google Scholar
21. Linnala A, Von Koskull H, Virtanen I. Isoforms of cellular fibronectin and tenascin in amniotic fluid. FEBS Lett1994;337:167–70.10.1016/0014-5793(94)80266-1Search in Google Scholar
22. Hirnle L, Kątnik-Prastowska I. EDA fibronectin isoform of amniotic fluid in relation to normal pregnancy stages and to pregnancies complicated by fetal postmaturity syndrome. Acta Obstet Gyn Scand. Submitted for publication.Search in Google Scholar
23. Faron G, Boulvain M, Irion O, Bernard P-M, Fraser WD. Prediction of preterm delivery by fetal fibronectin: a meta-analysis. Obstet Gynecol1998;92:153–8.10.1097/00006250-199807000-00030Search in Google Scholar
24. Leitich H, Egarter C, Kaider A, Hohlagschwandtner M, Berghammer P, Husslein P. Cervicovaginal fetal fibronectin as a marker for preterm delivery. A meta-analysis. Am J Obstet Gynecol1998;180:1169–76.10.1016/S0002-9378(99)70612-5Search in Google Scholar
25. Yoon BH, Romero R, Moon JB, Oh S-Y, Han S-Y, Kim JC, et al. The frequency and clinical significance of intra-amniotic inflammation in patients with a positive cervical fetal fibronectin. Am J Obstet Gynecol2001;185:1137–42.10.1067/mob.2001.118162Search in Google Scholar PubMed
26. Goffeng AR, Milsom I, Lindstedt G, ArneLundberg P, Andersch B. Fetal fibronectin in vaginal fluid of women in prolonged pregnancy. Gynecol Obstet Invest1997;44:224–8.10.1159/000291533Search in Google Scholar PubMed
27. Andrews WW, Goldenberg RL, Hauth JC. Preterm labour: emerging role of genital tract infections. Infect Agent Dis1995;4:196–211.Search in Google Scholar
28. Jacobsson B. Intra-amniotic infection and inflammation in preterm birth – is bacteria always the connection? Pediatr Res2005;57:473–4.10.1203/01.PDR.0000156475.50488.B0Search in Google Scholar PubMed
29. Maxwell NC, Davies PL, Kotecha S. Antenatal infection and inflammation: what's new? Curr Opin Infect Inflamm2006;19:253–8.10.1097/01.qco.0000224819.42729.2eSearch in Google Scholar PubMed
30. Romero R, Chaiworapongsa T, Espinoza J. Micronutrients and intrauterine infection, preterm birth and fetal inflammatory response syndrome. J Nutr2003;133:1668S–73.10.1093/jn/133.5.1668SSearch in Google Scholar PubMed
31. Ghezzi F, Franchi M, Raio L, Di Naro E, Bossi G, D'Eril GV, et al. Elevated amniotic fluid C-reactive protein at the time of genitic amniocentesis is a marker for preterm delivery. Am J Obstet Gynecol2002;186:268–73.10.1067/mob.2002.119628Search in Google Scholar PubMed
32. Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of amniotic fluid, intra-amniotic inflammation, and preterm delivery. Acta Obstet Gynecol Scand2005;84:551–7.10.1111/j.0001-6349.2005.00708.xSearch in Google Scholar PubMed
33. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature1970;227:680–5.10.1038/227680a0Search in Google Scholar PubMed
34. Knibbs R, Goldstein IJ, Ratcliff RM, Shibuya N. Characterization of the carbohydrate binding specificity of the leukoagglutinating lectin from Maackia amurensis. Comparison with other sialic acid-specific lectins. J Biol Chem1991;266:83–8.10.1016/S0021-9258(18)52405-4Search in Google Scholar
35. Shibuya N, Goldstein IJ, Broekaert WF, Nsimba-Lubaki M, Peeters B, Peumans WJ. The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac(α2,6)Gal/GalNAc sequence. J Biol Chem1987;262:1596–601.10.1016/S0021-9258(19)75677-4Search in Google Scholar
36. Yamashita K, Kochibe N, Ohkura T, Ueda I, Kobata A. Fractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin. J Biol Chem1985;260:4688–93.10.1016/S0021-9258(18)89125-6Search in Google Scholar
37. Yan L, Wilkins PP, Alvarez-Manilla G, Do SI, Smith DF, Cummings RD. Immobilized Lotus tetragonolobus agglutinin binds oligosaccharides containing the Le(x) determinant. Glycoconjugate J1997;14:45–55.10.1023/A:1018508914551Search in Google Scholar
38. Audette GF, Vandonselaar M, Delbaere LT. The 2.2 Å resolution structure of the O(H) blood-group-specific lectin I from Ulex europaeus. J Mol Biol2000;304:423–33.10.1006/jmbi.2000.4214Search in Google Scholar
39. Kątnik I, Jadach J, Krotkiewski H, Gerber J. Investigating the glycosylation of normal and ovarian cancer haptoglobins using digoxigenin-labeled lectins. Glycosyl Dis1994;1:97–104.10.1007/BF00919295Search in Google Scholar
40. Athayde N, Romero R, Maymon E, Gomez R, Pacora P, Yoon BH, et al. Interleukin 16 in pregnancy, parturition, rupture of fetal membranes, and microbial invasion of the amniotic cavity. Am J Obstet Gynecol2000;182:135–41.10.1016/S0002-9378(00)70502-3Search in Google Scholar
41. Orczyk-Pawiłowicz M, Floriański J, Zalewski J, Kątnik-Prastowska I. Relative amounts of sialic acid and fucose of amniotic fluid glycoconjugates in relation to pregnancy age. Glycoconjugate J2005;22:433–42.10.1007/s10719-005-4437-5Search in Google Scholar PubMed
©2007 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Associations between folate, vitamin B12, homocysteine and pathologies related to aging: the need to consider complex nutrient-nutrient and gene-nutrient interactions and the functional and socio-economic determinants in population-based studies
- Hyperhomocysteinemia and cardiovascular risk in postmenopausal women: the role of folate supplementation
- Association of vitamin B12, folate and homocysteine with functional and pathological characteristics of the elderly in a mountainous village in Sicily
- Association of thyroid dysfunction with vitamin B12, folate and plasma homocysteine levels in the elderly: a population-based study in Sicily
- Desmond (Des) Kenny MSc MCB FICI FRCPath Eur Clin Chem, 1941–2006
- Antibodies to citrullinated peptides: a significant step forward in the early diagnosis of rheumatoid arthritis
- Patient hydration: a major source of laboratory uncertainty
- Determination of the hepatitis C virus subtype: comparison of sequencing and reverse hybridization assays
- Quantification of relative changes in specific mRNAs from frozen whole blood – methodological considerations and clinical implications
- HER-2 protein concentrations in breast cancer cells increase before immunohistochemical and fluorescence in situ hybridization analysis turn positive
- Distinct alleles of mannose-binding lectin (MBL) and surfactant proteins A (SP-A) in patients with chronic cavitary pulmonary aspergillosis and allergic bronchopulmonary aspergillosis
- Novel molecular defect in the platelet ADP receptor P2Y12 of a patient with haemorrhagic diathesis
- Retrospective study of monoclonal gammopathies detected in the clinical laboratory of a Spanish healthcare district: 14-year series
- Renal impairment compromises the use of total homocysteine and methylmalonic acid but not total vitamin B12 and holotranscobalamin in screening for vitamin B12 deficiency in the aged
- Diagnostic value of calculated testosterone indices in the assessment of polycystic ovary syndrome
- Amniotic fibronectin fragmentation and expression of its domains, sialyl and fucosyl glycotopes associated with pregnancy complicated by intrauterine infection
- Predicted influence of sample hematocrit on injected mass of internal standard in mass spectrometry assays utilizing simple protein precipitation for sample preparation
- Evaluation of cardiovascular risk factors and related clinical markers in healthy young Japanese adults
- Cardiac natriuretic peptides after myocardial infarction: relationship with infarct size, left ventricular function and remodelling assessed by 99mTc-sestamibi gated-single photon emission tomography
- Plasma homocysteine cutoff values for venous thrombosis
- Lipid transfer protein activities in subjects with impaired glucose tolerance
- Heat shock protein 70-1 gene expression in pediatric heart surgery using blood cardioplegia
- Biochemical reference intervals for sex hormones with a new AutoDelfia method in aged men
- The Italian External Quality Control Programme for cystic fibrosis molecular diagnosis: 4 years of activity
- Standardization of ischemia-modified albumin testing: adjustment for serum albumin
- Is simultaneous measurement of anti-thyroid peroxidase and anti-thyroglobulin antibodies clinically useful in patients with thyroid dysfunction?
- To the Editor: In reply to Nakano et al. Clin Chem Lab Med 2006;44(5):522–532
- To the Editor: Author reply; Nakano et al. Clin Chem Lab Med 2006;44(5):522–532
- Accreditation of medical laboratories in the European Union
- Prevalence-dependent decision limits for the early detection of type 2 diabetes mellitus in venous blood, venous plasma and capillary blood during glucose challenge
Articles in the same Issue
- Associations between folate, vitamin B12, homocysteine and pathologies related to aging: the need to consider complex nutrient-nutrient and gene-nutrient interactions and the functional and socio-economic determinants in population-based studies
- Hyperhomocysteinemia and cardiovascular risk in postmenopausal women: the role of folate supplementation
- Association of vitamin B12, folate and homocysteine with functional and pathological characteristics of the elderly in a mountainous village in Sicily
- Association of thyroid dysfunction with vitamin B12, folate and plasma homocysteine levels in the elderly: a population-based study in Sicily
- Desmond (Des) Kenny MSc MCB FICI FRCPath Eur Clin Chem, 1941–2006
- Antibodies to citrullinated peptides: a significant step forward in the early diagnosis of rheumatoid arthritis
- Patient hydration: a major source of laboratory uncertainty
- Determination of the hepatitis C virus subtype: comparison of sequencing and reverse hybridization assays
- Quantification of relative changes in specific mRNAs from frozen whole blood – methodological considerations and clinical implications
- HER-2 protein concentrations in breast cancer cells increase before immunohistochemical and fluorescence in situ hybridization analysis turn positive
- Distinct alleles of mannose-binding lectin (MBL) and surfactant proteins A (SP-A) in patients with chronic cavitary pulmonary aspergillosis and allergic bronchopulmonary aspergillosis
- Novel molecular defect in the platelet ADP receptor P2Y12 of a patient with haemorrhagic diathesis
- Retrospective study of monoclonal gammopathies detected in the clinical laboratory of a Spanish healthcare district: 14-year series
- Renal impairment compromises the use of total homocysteine and methylmalonic acid but not total vitamin B12 and holotranscobalamin in screening for vitamin B12 deficiency in the aged
- Diagnostic value of calculated testosterone indices in the assessment of polycystic ovary syndrome
- Amniotic fibronectin fragmentation and expression of its domains, sialyl and fucosyl glycotopes associated with pregnancy complicated by intrauterine infection
- Predicted influence of sample hematocrit on injected mass of internal standard in mass spectrometry assays utilizing simple protein precipitation for sample preparation
- Evaluation of cardiovascular risk factors and related clinical markers in healthy young Japanese adults
- Cardiac natriuretic peptides after myocardial infarction: relationship with infarct size, left ventricular function and remodelling assessed by 99mTc-sestamibi gated-single photon emission tomography
- Plasma homocysteine cutoff values for venous thrombosis
- Lipid transfer protein activities in subjects with impaired glucose tolerance
- Heat shock protein 70-1 gene expression in pediatric heart surgery using blood cardioplegia
- Biochemical reference intervals for sex hormones with a new AutoDelfia method in aged men
- The Italian External Quality Control Programme for cystic fibrosis molecular diagnosis: 4 years of activity
- Standardization of ischemia-modified albumin testing: adjustment for serum albumin
- Is simultaneous measurement of anti-thyroid peroxidase and anti-thyroglobulin antibodies clinically useful in patients with thyroid dysfunction?
- To the Editor: In reply to Nakano et al. Clin Chem Lab Med 2006;44(5):522–532
- To the Editor: Author reply; Nakano et al. Clin Chem Lab Med 2006;44(5):522–532
- Accreditation of medical laboratories in the European Union
- Prevalence-dependent decision limits for the early detection of type 2 diabetes mellitus in venous blood, venous plasma and capillary blood during glucose challenge
