Home Anti-thyroid-stimulating hormone receptor antibodies determined by second-generation assay
Article
Licensed
Unlicensed Requires Authentication

Anti-thyroid-stimulating hormone receptor antibodies determined by second-generation assay

  • Amani Mankaï , Dorsaf Toumi , Molka Chadli-Chaieb , Leila Ghedira-Besbes , Fathia Saad , Mohamed Ouertani , Habib Sfar , Moncef Jeddi , Larbi Chaieb and Ibtissem Ghedira
Published/Copyright: January 1, 2007
Become an author with De Gruyter Brill
Submit Manuscript Author Information
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 45 Issue 1

Abstract

Background: The aim of our study was to determine the frequency of anti-thyroid-stimulating hormone (TSH) receptor antibodies (TRAb) in Tunisian patients with Graves' disease (GD) and to compare the validity of TRAb to that of thyroperoxidase (TPO-Ab) and thyroglobulin antibodies (TG-Ab).

Methods: ELISA was used to determine the frequency of TRAb, TPO-Ab and TG-Ab in sera of 190 patients with GD. Patients were divided into four groups: those with untreated active GD (group A, n=71), those receiving treatment with anti-thyroid drugs (group B, n=85), those in relapse (group C, n=15) and those in remission (group D, n=19). Sera of 100 healthy blood donors served as controls.

Results: The sensitivity of TRAb for the diagnosis of GD (95.8%) was significantly higher than that of TPO-Ab (73.2%) and TG-Ab (42.2%) (p=0.0005 and p<10–7, respectively). The positive rate for TRAb was lower in group B than in group A (70.6% and 95.8%, respectively; p=0.0001). The levels of TRAb were significantly higher in group A than in group B (mean 30.1 and 14.2 IU/L, respectively; p=0.006).

Conclusions: TRAb, but neither TPO-Ab nor TG-Ab, is valuable in the diagnosis and management of patients with GD.

Clin Chem Lab Med 2007;45:26–9.

Keywords: ELISA; Graves' disease; thyroglobulin antibodies; thyroperoxydase antibodies; TSH receptor antibodies
:
Corresponding author: Ibtissem Ghedira, Laboratory of Immunology, Farhat Hached Hospital, Avenue Ibn El Jazzar, 4000 Sousse, Tunisia Phone: +216-73221411, Fax: +216-73226702

References

1. Schott M, Scherbaum WA, Morgenthaler NG. Thyrotropin receptor autoantibodies in Graves' disease. Trends Endocrinol Metab2005;16:243–8.10.1016/j.tem.2005.05.009Search in Google Scholar

2. Ando T, Latif R, Davies TF. Thyrotropin receptor antibodies: new insights into their actions and clinical relevance. Best Pract Res Clin Endocrinol Metab2005;19:33–52.10.1016/j.beem.2004.11.005Search in Google Scholar

3. Smith BR. Thyroid autoantibodies. Scand J Clin Lab Invest2001;61:45–52.10.1080/003655101753352040Search in Google Scholar

4. Orgiazzi J. Anti-TSH receptor antibodies in clinical practice. Endocrinol Metab Clin North Am2000;29:339–55.10.1016/S0889-8529(05)70135-3Search in Google Scholar

5. Orgiazzi J, Madec AM, Ducottet X. The role of stimulating, function-blocking and growth-blocking anti-TSH receptor antibodies (TRAbs) in GD, Hashimoto's disease and in atrophic thyroiditis. Ann Endocrinol2003;64:31–6.Search in Google Scholar

6. Costagliola S, Morgenthaler NG, Hoermann R, Badenhoop K, Struck J, Freitag D, et al. Second generation assay for thyrotropin receptor antibodies has superior diagnostic sensitivity for Graves' disease. J Clin Endocrinol Metab1999;84:90–7.10.1210/jcem.84.1.5415Search in Google Scholar PubMed

7. Smith BR, Bolton J, Young S, Collyer A, Weeden A, Bradbury J, et al. A new assay for thyrotropin receptor autoantibodies. Thyroid2004;14:830–5.10.1089/1050725042451248Search in Google Scholar

8. Kamijo K. TSH-receptor antibody measurement in patients with various thyrotoxicosis and Hashimoto's thyroiditis: a comparison of two two-step assays, coated plate ELISA using porcine TSH-receptor and coated tube radioassay using human recombinant TSH-receptor. Endocr J2003;50:113–6.10.1507/endocrj.50.113Search in Google Scholar PubMed

9. Kamijo K, Ishikawa K, Tanaka M. Clinical evaluation of 3rd generation assay for thyrotropin receptor antibodies: the M22-biotin-based ELISA initiated by Smith. Endocr J2005;52:525–9.10.1507/endocrj.52.525Search in Google Scholar PubMed

10. Maugendre D, Massart C. Clinical value of a new TSH binding inihibitory activity assay using human TSH receptors in the follow-up of antithyroid drug treated Graves' disease. Comparison with thyroid stimulating antibody bioassay. Clin Endocrinol2001;54:89–96.10.1046/j.1365-2265.2001.01197.xSearch in Google Scholar PubMed

11. Shibayama K, Ohyama Y, Yokota Y, Ohtsu S, Takubo N, Matsuura N. Assays for thyroid-stimulating antibodies and thyrotropin-binding inhibitory immunoglobulins in children with Graves' disease. Endocr J2005;52:505–10.10.1507/endocrj.52.505Search in Google Scholar PubMed

12. Alnaqdy A, Al-Maskari M. Determination of the levels of anti-thyroid-stimulating hormone receptor antibody with thyroid peroxidase antibody in Omani patients with Graves' disease. Med Princ Pract2005;14:209–12.10.1159/000085736Search in Google Scholar PubMed

13. Schott M, Morgenthaler NG, Fritzen R, Feldkamp J, Willenberg HS, Scherbaum WA, et al. Levels of autoantibodies against human TSH receptor predict relapse of hyperthyroidism in Graves' disease. Horm Metab Res2004;36:92–6.10.1055/s-2004-814217Search in Google Scholar PubMed

14. Quadbeck B, Hoermann R, Hahn S, Roggenbuck U, Mann K, Janssen OE. Binding, stimulating and blocking TSH receptor antibodies to the thyrotropin receptor as predictors of relapse of Graves' disease after withdrawal of antithyroid treatment. Horm Metab Res2005;37:745–50.10.1055/s-2005-921102Search in Google Scholar PubMed

15. Quadbeck B, Hoermann R, Roggenbuck U, Hahn S, Mann K, Janssen OE; Basedow Study Group. Sensitive thyrotropin and thyrotropin-receptor antibody determinations one month after discontinuation of antithyroid drug treatment as predictors of relapse in Graves' disease. Thyroid2005;15:1047–54.10.1089/thy.2005.15.1047Search in Google Scholar PubMed

16. Hu YM, Liu W, Lu GH. Significance of combining tests of thyroid autoantibodies in differential diagnosis between Graves' disease and Hashimoto's hyperthyroidism. Zhonghua Yi Xue Za Zhi2003;83:940–2.Search in Google Scholar

17. Giovanella L, Ceriani L, Garancini S. Evaluation of the 2nd generation radio-receptional assay for anti-TSH receptor antibodies (TRAb) in autoimmune thyroid diseases. Comparison with 1st generation and anti-thyroperoxidase antibodies (AbTPO). Q J Nucl Med2001;45:115–9.Search in Google Scholar

18. Lian XL, Bai Y, Sun ML, Guo ZS, Dai WX. Clinical validity of anti-thyroperoxidase antibody and anti-thyroglobulin antibody. Zhongguo Yi Xue Ke Xue Yuan Xue Bao2004;26:677–81.Search in Google Scholar

Published Online: 2007-01-01
Published in Print: 2007-01-01

©2007 by Walter de Gruyter Berlin New York

Articles in the same Issue

  1. CCLM: Bringing advances in laboratory medicine to the “New World”
  2. Diagnostic approach to inherited bleeding disorders
  3. Linking laboratory and medication data: new opportunities for pharmacoepidemiological research
  4. Association between polymorphisms of ACE, B2AR, ANP and ENOS and cardiovascular diseases: a community-based study in the Matsu area
  5. Anti-thyroid-stimulating hormone receptor antibodies determined by second-generation assay
  6. Serum levels of granulocyte colony-stimulating factor (G-CSF) and macrophage colony-stimulating factor (M-CSF) in pancreatic cancer patients
  7. Surrogate markers of insulin resistance in assessing individuals with new categories “prehypertension” and “prediabetes”
  8. Evaluation of pre-analytical, demographic, behavioural and metabolic variables on fibrinolysis and haemostasis activation markers utilised to assess hypercoagulability
  9. Hyperhomocysteinaemia and immune activation in patients with cancer
  10. The effect of homocysteine reduction by B-vitamin supplementation on inflammatory markers
  11. Lipid peroxidation in Down syndrome caused by regular trisomy 21, trisomy 21 by Robertsonian translocation and mosaic trisomy 21
  12. Pregnancy-associated plasma protein A in dialysis patients
  13. L-Cysteine supplementation prevents exercise-induced alterations in human erythrocyte membrane acetylcholinesterase and Na+,K+-ATPase activities
  14. Assessment of homocysteine, neopterin and nitric oxide levels in Behcet's disease
  15. Increased adenosine deaminase in hydatidiform mole
  16. Classification tree analysis for the discrimination of pleural exudates and transudates
  17. External quality assessment of hemoglobin A2 measurement: data from an Italian pilot study with fresh whole blood samples and commercial HPLC systems
  18. Comparison of four commercial quantitative HIV-1 assays for viral load monitoring in clinical daily routine
  19. Interchangeability of measurements of CA 19-9 in serum with four frequently used assays: an update
  20. Analytical validation of the new version of the Liaison N-Tact PTH assay
  21. Validation of an automated sensitive immunoassay for quantitation of cytokines in the sputum of cystic fibrosis patients
  22. Strong interference of hemoglobin concentration on CSF total protein measurement using the trichloroacetic acid precipitation method
  23. Correction of patient results for Beckman Coulter LX-20 assays affected by interference due to hemoglobin, bilirubin or lipids: a practical approach
  24. Acquiring a measurement system within the framework of standard ISO 15189
  25. Six Sigma and laboratory consultation
  26. ESR or LSRB, that is the question!
  27. Reply to the Letter written by Hardeman
  28. Natriuretic peptides. The hormones of the heart
https://doi.org/10.1515/CCLM.2007.016
Keywords for this article
ELISA; Graves' disease; thyroglobulin antibodies; thyroperoxydase antibodies; TSH receptor antibodies

Articles in the same Issue

  1. CCLM: Bringing advances in laboratory medicine to the “New World”
  2. Diagnostic approach to inherited bleeding disorders
  3. Linking laboratory and medication data: new opportunities for pharmacoepidemiological research
  4. Association between polymorphisms of ACE, B2AR, ANP and ENOS and cardiovascular diseases: a community-based study in the Matsu area
  5. Anti-thyroid-stimulating hormone receptor antibodies determined by second-generation assay
  6. Serum levels of granulocyte colony-stimulating factor (G-CSF) and macrophage colony-stimulating factor (M-CSF) in pancreatic cancer patients
  7. Surrogate markers of insulin resistance in assessing individuals with new categories “prehypertension” and “prediabetes”
  8. Evaluation of pre-analytical, demographic, behavioural and metabolic variables on fibrinolysis and haemostasis activation markers utilised to assess hypercoagulability
  9. Hyperhomocysteinaemia and immune activation in patients with cancer
  10. The effect of homocysteine reduction by B-vitamin supplementation on inflammatory markers
  11. Lipid peroxidation in Down syndrome caused by regular trisomy 21, trisomy 21 by Robertsonian translocation and mosaic trisomy 21
  12. Pregnancy-associated plasma protein A in dialysis patients
  13. L-Cysteine supplementation prevents exercise-induced alterations in human erythrocyte membrane acetylcholinesterase and Na+,K+-ATPase activities
  14. Assessment of homocysteine, neopterin and nitric oxide levels in Behcet's disease
  15. Increased adenosine deaminase in hydatidiform mole
  16. Classification tree analysis for the discrimination of pleural exudates and transudates
  17. External quality assessment of hemoglobin A2 measurement: data from an Italian pilot study with fresh whole blood samples and commercial HPLC systems
  18. Comparison of four commercial quantitative HIV-1 assays for viral load monitoring in clinical daily routine
  19. Interchangeability of measurements of CA 19-9 in serum with four frequently used assays: an update
  20. Analytical validation of the new version of the Liaison N-Tact PTH assay
  21. Validation of an automated sensitive immunoassay for quantitation of cytokines in the sputum of cystic fibrosis patients
  22. Strong interference of hemoglobin concentration on CSF total protein measurement using the trichloroacetic acid precipitation method
  23. Correction of patient results for Beckman Coulter LX-20 assays affected by interference due to hemoglobin, bilirubin or lipids: a practical approach
  24. Acquiring a measurement system within the framework of standard ISO 15189
  25. Six Sigma and laboratory consultation
  26. ESR or LSRB, that is the question!
  27. Reply to the Letter written by Hardeman
  28. Natriuretic peptides. The hormones of the heart
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 13.9.2025 from https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2007.016/html
Scroll to top button