Fifteen polymorphisms in endothelin-1, endothelin-2 and endothelin-receptor-A genotyped by four duplex assays and seven simple assays on a LightCycler using hybridization probes
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Farhad Arjomand-Nahad
Abstract
Endothelins (EDNs) are peptides, produced by various tissues, with potent vasoactive and mitogenic properties. Endothelin actions are mediated via specific G protein-coupled receptors of two subtypes, endothelin-receptor-A (EDNRA) and endothelin-receptor-B (EDNRB). Some polymorphisms of the EDN1, EDN2 and EDNRA genes may influence susceptibility to vascular diseases. Thus, genotyping for polymorphisms of these genes may represent a tool for predicting individual susceptibility to vascular diseases. Here, we report 11 fluorescence resonance energy transfer (FRET) assays for the detection of 15 polymorphisms, because the assays used in previous studies (allele-specific PCR and restriction fragment-length polymorphism assays) for some of these polymorphisms are laborious and time-consuming. The newly developed assays are fast and work without expensive ready-to-use mixtures.
Clin Chem Lab Med 2006;44:929–32.
References
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©2006 by Walter de Gruyter Berlin New York
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- Laboratory medicine in the 2000s: programmed death or rebirth?
- Polymorphisms of the inflammatory system and risk of ischemic cerebrovascular events
- Haptoglobin polymorphism in patients with preeclampsia
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- A robust liquid chromatography tandem mass spectrometry method for total plasma homocysteine determination in clinical practice
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