Oxidative stress: potential of distinct peroxide determination systems
-
Meinrad Lindschinger
, Karl Nadlinger , Norbert Adelwöhrer , Karin Holweg , Markus Wögerbauer , Jörg Birkmayer , Karl-Heinz Smolle and Willibald Wonisch
Abstract
When reactive oxygen species attack biological structures, peroxides, which are short-lived oxidative intermediates, are generated. We evaluated the potential of two different, commercially available peroxide activity assays (Pox-Act and d-ROMS) to see whether the results were associated with the clinical condition of subjects who were participating in a routine health care program. Furthermore, we determined the total antioxidant status (TAS) and the titer of autoantibodies against oxidized low-density lipoprotein (oLAb) to verify the hydroperoxide measurements.
Subjects with medical conditions (hereafter referred to as patients) had significantly increased serum peroxide levels compared to healthy subjects. The d-ROMS kit indicated that 86% of subjects had an increased level of total peroxides. Although the assays had a significant correlation (p < 0.001), 34% of the subjects had an increased total peroxide concentration in the Pox-Act assay that was clearly associated with clinical symptoms. Furthermore, the sensitivity of the Pox-Act assay was 35 times higher than that of the d-ROMS kit. In subjects with medical conditions, there was a trend toward a decreased TAS and a slightly increased oLAb titer in comparison to healthy subjects, but this was not statistically significant. The Pox-Act assay seems to be a valuable tool for the determination of total peroxides, while the results from the d-ROMS kit should be considered with caution.
References
1 Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 1991; 11:81–128.10.1016/0891-5849(91)90192-6Search in Google Scholar
2 Schaur RJ. Basic aspects of the biochemical reactivity of 4-hydroxynonenal. Mol Aspects Med 2003; 24:149–59.10.1016/S0098-2997(03)00009-8Search in Google Scholar
3 Siems W, Grune T. Intracellular metabolism of 4-hydroxynonenal. Mol Aspects Med 2003; 24:167–75.10.1016/S0098-2997(03)00011-6Search in Google Scholar
4 Zarkovic N. 4-Hydroxynonenal as a bioactive marker of pathophysiological processes. Mol Aspects Med 2003; 24:281–91.10.1016/S0098-2997(03)00023-2Search in Google Scholar
5 Esterbauer H. Estimation of peroxidative damage. A critical review. Pathol Biol (Paris) 1996; 44:25–8.Search in Google Scholar
6 Cao G, Prior RL. Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem 1998; 44:1309–15.10.1093/clinchem/44.6.1309Search in Google Scholar
7 Rice-Evans C, Miller NJ. Total antioxidant status in plasma and body fluids. Methods Enzymol 1994; 234:279–93.10.1016/0076-6879(94)34095-1Search in Google Scholar
8 Jialal I, Devaraj S. Low-density lipoprotein oxidation, antioxidants, and atherosclerosis: a clinical biochemistry perspective. Clin Chem 1996; 42:498–506.10.1093/clinchem/42.4.498Search in Google Scholar
9 Lands LC, Grey VL, Grenier C. Total plasma antioxidant capacity in cystic fibrosis. Pediatr Pulmonol 2000; 29:81–7.10.1002/(SICI)1099-0496(200002)29:2<81::AID-PPUL1>3.0.CO;2-NSearch in Google Scholar
10 Lantos J, Roth E, Czopf L, Nemes J, Gal I. Monitoring of plasma total antioxidant status in different diseases. Acta Chir Hung 1997; 36:188–9.Search in Google Scholar
11 Child RB, Wilkinson DM, Fallowfield JL., Donnelly AE. Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to a simulated half-marathon run. Med Sci Sports Exerc 1998; 30:1603–7.10.1097/00005768-199811000-00008Search in Google Scholar
12 MacKinnon KL, Molnar Z, Lowe D, Watson ID, Shearer E. Measures of total free radical activity in critically ill patients. Clin Biochem 1999; 32:263–8.10.1016/S0009-9120(98)00109-XSearch in Google Scholar
13 Winkler R, Griebenow S, Wonisch W. Effect of iodide on total antioxidant status of human serum. Cell Biochem Funct 2000; 18:143–6.10.1002/(SICI)1099-0844(200006)18:2<143::AID-CBF857>3.0.CO;2-#Search in Google Scholar
14 Krstevska M, Dzhekova-Stojkova S, Bosilkova G. Menopause, coronary artery disease and antioxidants. Clin Chem Lab Med 2001; 39:641–4.10.1515/CCLM.2001.104Search in Google Scholar
15 Opara EC, Abdel-Rahman E, Soliman S, Kamel WA, Souka S, Lowe JE, et al. Depletion of total antioxidant capacity in type 2 diabetes. Metabolism 1999; 48:1414–7.10.1016/S0026-0495(99)90152-XSearch in Google Scholar
16 Napoli C. Low density lipoprotein oxidation and atherogenesis: from experimental models to clinical studies. G Ital Cardiol 1997; 27:1302–14.Search in Google Scholar
17 Vaarala O. Antibodies to oxidised LDL. Lupus 2000; 9:202–5.10.1191/096120300678828280Search in Google Scholar
18 Inoue T, Uchida T, Kamishirado H, Takayanagi K, Hayashi T, Morooka S. Clinical significance of antibody against oxidized low density lipoprotein in patients with atherosclerotic coronary artery disease. J Am Coll Cardiol 2001; 37:775–9.10.1016/S0735-1097(00)01199-2Search in Google Scholar
19 Buonocore G, Perrone S, Longini M, Terzuoli L, Bracci R. Total hydroperoxide and advanced oxidation protein products in preterm hypoxic babies. Pediatr Res 2000; 47:221–4.10.1203/00006450-200002000-00012Search in Google Scholar
20 Poli G, Schaur RJ. 4-Hydroxynonenal in the pathomechanisms of oxidative stress. Life 2000; 50:315–21.Search in Google Scholar
21 Felley CP, Pignatelli B, Van Melle GD, Crabtree JE, Stolte M, Diezi J, et al. Oxidative stress in gastric mucosa of asymptomatic humans infected with Helicobacter pylori: effect of bacterial eradication. Helicobacter 2002; 7:342–8.10.1046/j.1523-5378.2002.00107.xSearch in Google Scholar
22 Bostan M, Brasoveanu LI, Livescu A, Manda G, Neagu M, Iordachescu D. Effects of synovial fluid on respiratory burst of granulocytes in rheumatoid arthritis. J Cell Mol Med 2001; 5:188–94.10.1111/j.1582-4934.2001.tb00152.xSearch in Google Scholar
23 Niess AM, Dickhuth HH, Northoff H, Fehrenbach E. Free radicals and oxidative stress in exercise – immunological aspects. Exerc Immunol Rev 1999; 5:22–56.Search in Google Scholar
24 Riserus U, Basu S, Jovinge S, Fredrikson GN, Arnlov J, Vessby B. Supplementation with conjugated linoleic acid causes isomer-dependent oxidative stress and elevated C-reactive protein: a potential link to fatty acid-induced insulin resistance. Circulation 2002; 106:1925–9.10.1161/01.CIR.0000033589.15413.48Search in Google Scholar
25 Festa A, Kopp HP, Menzel EJ. Autoantibodies to oxidised low density lipoproteins in IDDM are inversely related to metabolic control and microvascular complications. Diabetologia 1998; 41:350–60.10.1007/s001250050914Search in Google Scholar
26 Cesarone MR, Belcaro G, Caratelli M, Cornelli U, De Sanctis MT, Incandela L, et al. A simple assay to monitor oxidative stress. Int Angiol 1999; 18:127–30.Search in Google Scholar
27 Elstner EF. Der Sauerstoff: Die biologische OH•-Bildung. Mannheim-Wien-Zürich: Wissenschaftsverlag, 1990.Search in Google Scholar
28 Minotti G, Aust SD. The role of iron in the initiation of lipid peroxidation. Chem Phys Lipids 1987; 44:191–208.10.1016/0009-3084(87)90050-8Search in Google Scholar
29 Parmigiani S, Gambini L, Massari A, Pezzani I, Payer C, Bevilacqua G. Evaluation of reactive oxygen metabolites with micromethod in neonates: determination of standards of normality in full-term babies. Acta Biomed Ateneo Parmense 1997; 68Suppl 1:103–6.Search in Google Scholar
30 Digiesi V, Oliviero C, Gianno V, Rossetti M, Fiorillo C, Oradei A, et al. Reactive metabolites of oxygen, lipid peroxidation, total antioxidant capacity and vitamin E in essential arterial hypertension. Clin Ter 1997; 148:515–9.Search in Google Scholar
31 Tatzber F, Griebenow S, Wonisch W, Winkler R. Dual method for the determination of peroxidase-activity and total peroxides – iodide leads to a significant increase of peroxidase activity in human sera. Anal Biochem 2003; 316:147–53.10.1016/S0003-2697(02)00652-8Search in Google Scholar
32 Schippinger G, Wonisch W, Abuja PM, Fankhauser F, Winklhofer-Roob BM, Halwachs G. Lipid peroxidation and antioxidant status in professional American football players during competition. Eur J Clin Invest 2002; 32:686–92.10.1046/j.1365-2362.2002.01021.xSearch in Google Scholar
33 Roob JM, Khoschsorur G, Tiran A, Horina JH, Holzer H, Winklhofer-Roob BM. Vitamin E attenuates oxidative stress induced by intravenous iron in patients on hemodialysis. J Am Soc Nephrol 2000; 11:539–49.10.1681/ASN.V113539Search in Google Scholar
34 Tatzber F, Esterbauer H. Autoantibodies to oxidized low density lipoprotein. In: Bellomo G, Finardi G, Maggi E, Rice-Evans C, editors. Free radicals IX. London: Richelieu Press, 1995:245–62.Search in Google Scholar
35 Thomas L, Abdelhamid S. Diagnostische Sensitivität und Spezifität. In: Thomas L, editor. Labor und Diagnose, 4th ed. Frankfurt: Medizinische Verlagsgesellschaft Marburg, 1995:1863–4.Search in Google Scholar
36 O’Connell MJ, Peters TJ. Ferritin and haemosiderin in free radical generation, lipid peroxidation and protein damage. Chem Phys Lipids 1987; 45:241–9.10.1016/0009-3084(87)90067-3Search in Google Scholar
37 Hostmark AT, Lystad E, Vellar OD, Hovi K, Berg JE. Reduced plasma fibrinogen, serum peroxides, lipids, and apolipoproteins after a 3-week vegetarian diet. Plant Foods Hum Nutr 1993; 43:55–61.10.1007/BF01088096Search in Google Scholar
38 McLemore JL, Beeley P, Thorton K, Morrisroe K, Blackwell W, Dasgupta A. Rapid automated determination of lipid hydroperoxide concentrations and total antioxidant status of serum samples from patients infected with HIV: elevated lipid hydroperoxide concentrations and depleted total antioxidant capacity of serum samples. Am J Clin Pathol 1998; 109:268–73.10.1093/ajcp/109.3.268Search in Google Scholar
39 Szymczyk G, Beltowski J, Marciniak A, Kotarski J. Assessment of serum lipid peroxide levels and antioxidant status in females who had undergone total abdominal hysterectomy without closing of the peritoneum. Ginekol Pol 2003; 74:1397–403.Search in Google Scholar
40 Agil A, Fuller CJ, Jialal I. Susceptibility of plasma to ferrous iron/hydrogen peroxide-mediated oxidation: demonstration of a possible Fenton reaction. Clin Chem 1995; 41:220–5.10.1093/clinchem/41.2.220Search in Google Scholar
41 Esterbauer H, Benedetti A, Lang J, Fulceri R, Fauler G, Comporti M. Studies on the mechanism of formation of 4-hydroxynonenal during microsomal lipid peroxidation. Biochim Biophys Acta 1986; 876:154–66.10.1016/0005-2760(86)90329-2Search in Google Scholar
42 Herrero A, Portero-Otin M, Bellmunt MJ, Pamplona R, Barja G. Effect of the degree of fatty acid unsaturation of rat heart mitochondria on their rates of H2O2 production and lipid and protein oxidative damage. Mech Ageing Dev 2001; 122:427–43.10.1016/S0047-6374(01)00214-7Search in Google Scholar
43 Gieseg S, Duggan S, Gebicki JM. Peroxidation of proteins before lipids in U937 cells exposed to peroxyl radicals. Biochem J 2000; 350:215–8.10.1042/bj3500215Search in Google Scholar
44 Garcia-Ruiz C, Colell A, Morales A, Kaplowitz N, Fernandez-Checa JC. Role of oxidative stress generated from the mitochondrial electron transport chain and mitochondrial glutathione status in loss of mitochondrial function and activation of transcription factor nuclear factor-kappa B: studies with isolated mitochondria and rat hepatocytes. Mol Pharmacol 1995; 48:825–34.Search in Google Scholar
45 Wonisch W, Hayn M, Schaur RJ, Tatzber F, Kranner I, Grill D, et al. Increased stress parameter synthesis in the yeast Saccharomyces cerevisiae after treatment with 4-hydroxy-2-nonenal. FEBS Lett 1997; 405:11–5.10.1016/S0014-5793(97)00123-3Search in Google Scholar
46 Prior RL, Cao G. In vivo total antioxidant capacity: comparison of different analytical methods. Free Radic Biol Med 1999; 27:1173–81.10.1016/S0891-5849(99)00203-8Search in Google Scholar
47 Brown S, Georgatos M, Reifel C, Song JH, Shin SH, Hong M. Recycling processes of cellular ascorbate generate oxidative stress in pancreatic tissues in in vitro system. Endocrine 2002; 18:91–6.10.1385/ENDO:18:1:91Search in Google Scholar
48 Stocker R. The ambivalence of vitamin E in atherogenesis. Trends Biochem Sci 1999; 24:219–23.10.1016/S0968-0004(99)01404-8Search in Google Scholar
49 Bowen HT, Omaye ST. Oxidative changes associated with beta-carotene and alpha-tocopherol enrichment of human low-density lipoproteins. J Am Coll Nutr 1998; 17:171–9.10.1080/07315724.1998.10718743Search in Google Scholar
50 Stanger O, Semmelrock HJ, Wonisch W, Bös U, Pabst E, Wascher TC. Effects of folate treatment and homocysteine lowering on resistance vessel reactivity in atherosclerotic subjects. J Pharmacol Exp Ther 2002; 303:158–62.10.1124/jpet.102.036715Search in Google Scholar
51 Wildburger R, Borovic S, Zarkovic N, Tatzber F. Post-traumatic dynamic changes in the antibody titer against oxidized low density lipoproteins. Wien Klin Wochenschr 2000; 112:798–803.Search in Google Scholar
52 Luc G, Fruchart JC. Oxidation of lipoproteins and atherosclerosis. Am J Clin Nutr 1991; 53(1 Suppl):206S–9S.10.1093/ajcn/53.1.206SSearch in Google Scholar
53 Inoue T, Inoue K, Maeda H, Takayanagi K, Morooka S. Immunological response to oxidized LDL occurs in association with oxidative DNA damage independently of serum LDL concentrations in dyslipidemic patients. Clin Chim Acta 2001; 305:115–21.10.1016/S0009-8981(00)00426-5Search in Google Scholar
54 Pincemail J, Lecomte J, Castiau JP, Collard E, Vasankari T, Cheramy-Bien JP, et al. Evaluation of autoantibodies against oxidized LDL and antioxidant status in top soccer and basketball players after 4 months of competition. Free Radic Biol Med 2000; 28:559–65.10.1016/S0891-5849(99)00259-2Search in Google Scholar
55 Steinerova A, Stozicky F, Racek J, Tatzber F, Zima T, Stetina R. Antibodies against oxidized LDL in infants. Clin Chem 2001; 47:1137–8.10.1093/clinchem/47.6.1137Search in Google Scholar
56 Shoji T, Nishizawa Y, Fukumoto M, Shimamura K, Kimura J, Kanda H, et al. Inverse relationship between circulating oxidized low density lipoprotein (oxLDL) and anti-oxLDL antibody levels in healthy subjects. Atherosclerosis 2000; 148:171–7.10.1016/S0021-9150(99)00218-XSearch in Google Scholar
57 Strohmaier W, Reiger J, Tatzber F, Wonisch W, Schlag G, Esterbauer H. Septicemic patients show a decrease in neopterin levels and an increase in autoantibodies to oxidized LDL (oLAb) after successful therapeutic intervention. Biofactors 1997; 6:279–80.Search in Google Scholar
58 Schumacher M, Eber B, Tatzber F, Kaufmann P, Halwachs G, Fruhwald FM, et al. Transient reduction of autoantibodies against oxidized LDL in patients with acute myocardial infarction. Free Radic Biol Med 1995; 18:1087–91.10.1016/0891-5849(94)00216-7Search in Google Scholar
59 Winklhofer-Roob BM, Van’t Hof MA, Shmerling DH. Reference values for plasma concentrations of vitamin E and A and carotenoids in a Swiss population from infancy to adulthood, adjusted for seasonal influences. Clin Chem 1997; 43:146–53.10.1093/clinchem/43.1.146Search in Google Scholar
© Walter de Gruyter
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Articles in the same Issue
- Rapid detection of the factor XIII Val34Leu (163 G→T) polymorphism by real-time PCR using fluorescence resonance energy transfer detection and melting curve analysis
- Changes of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in the serum of patients with autoimmune diseases: association with age and disease activity
- A recombinant cell bioassay for measurement of overall estrogenic activity of serum: preliminary results in women with breast cancer
- N-terminal pro-atrial natriuretic peptide as a biochemical marker of long-term interventional success after radiofrequency catheter ablation of paroxysmal supraventricular tachyarrhythmias
- Preventing in vitro lipoperoxidation in the malondialdehyde-thiobarbituric assay
- Oxidative stress: potential of distinct peroxide determination systems
- Quality assessment in cytogenetic and molecular genetic testing: the experience of the Italian Project on Standardisation and Quality Assurance
- Guidelines for blood smear preparation and staining procedure for setting up an external quality assessment scheme for blood smear interpretation. Part I: control material
- Measurement of serum and plasma osmolality in healthy young humans – influence of time and storage conditions
- High in-hospital mortality of intensive care patients with nucleated red blood cells in blood
- Frequency of –163 C > A and 63 C > G single nucleotide polymorphism of cytochrome P450 1A2 in two African populations
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- Plasma or serum samples: measurements of cardiac troponin T and of other analytes compared
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- Comparison of serum digoxin concentration monitoring by fluorescence polarization immunoassay on the TDxFLx® and dry chemistry enzyme immunoassay on the Vitros 950
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- Analytical evaluation and reference values of serum amyloid-A on the BN ProSpec
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- Analysis of γ-globulins consisting of hepatitis C-associated cryoglobulins in the blood
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- Santorini Biologie Prospective Conference 2004 “From Human Genetic Variations to Prediction of Risks and Responses to the Environment”, Santorini, Greece, September 30–October 4, 2004