Guidelines for blood smear preparation and staining procedure for setting up an external quality assessment scheme for blood smear interpretation. Part I: control material
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Juan-Lluis Vives Corrons
Abstract
Blood smear analysis is a well known technique in medical laboratories. Clinical relevance of this analysis and its interpretation are very important. Consequently, monitoring of laboratory performance by an external quality assessment scheme is strongly recommended. Most starting external quality organizers set up a scheme for clinical chemistry. Due to a lack of guidance documents, many organizers are reluctant to offer a hematology scheme. This article aims to be a very practical guidance document for external quality assessment organizers for the set up of blood smear schemes.
References
1 National Committee for Clinical Laboratory Standards. Reference leukocyte differential count (proportional) and evaluation of instrumental methods. Villanova, PA: NCCLS approved standard document H20-A, 1992.Search in Google Scholar
2 Lewis SM. Blood film evaluations as a quality control activity. Clin Lab Haematol 1990; 12(Suppl 1):119–27.Search in Google Scholar
3 Bentley SA. Quality control and the differential leukocyte count. Clin Lab Haematol 1990; 12(Suppl 1):101–9.Search in Google Scholar
4 Houwen B. Blood film preparation and staining procedures. In: Pierre RV, editor. Clinics in laboratory medicine. Philadelphia: Saunders, 2002;22:1–14.Search in Google Scholar
5 National Committee for Clinical Laboratory Standards. Evacuated tubes and additives for blood specimen collection. Villanova, PA: NCCLS approved standard document H1–A4, 1996.Search in Google Scholar
6 National Committee for Clinical Laboratory Standards. Devices for collection of skin puncture blood specimens. Villanova, PA: NCCLS approved guideline document H14–A2, 1990.Search in Google Scholar
7 National Committee for Clinical Laboratory Standards. Procedures for the collection of diagnostic blood specimens by venipuncture. Villanova, PA: NCCLS approved standard document H3–A4, 1998.Search in Google Scholar
8 National Committee for Clinical Laboratory Standards. Procedures for the collection of diagnostic blood specimens by skin puncture. Villanova, PA: NCCLS approved standard document H4–A3, 1991.Search in Google Scholar
9 Bonte HA, Hoorn RKJ. Recommendations for the procedure and terminology of the microscopic examination of peripheral blood. Society for Hematological Laboratory Diagnostics, 1990Search in Google Scholar
10 International Council for Standardization in Hematology (ICSH). Recommendations of the ICSH for EDTA anticoagulation of blood for blood cell counting and sizing. Am J Clin Pathol 1993; 100:371.10.1093/ajcp/100.4.371Search in Google Scholar PubMed
11 Dacie JV, Lewis SM. Practical haematology, 9th ed. Edinburgh, Scotland: Churchill Livingstone, 2001.Search in Google Scholar
12 Hull BP, Bakhiet N, Ryu J, Houwen B. Biohazard evaluation of laboratory hematology tests. Presented at the 7th International Symposium on Technological Innovations in Laboratory Hematology, Lake Tahoe, NV, 1994.Search in Google Scholar
13 Stiene-Martin EA. Causes for poor leukocyte distribution in manual spreader-slide blood films. Am J Med Technol 1980; 46:624–32.Search in Google Scholar
14 Born JW, Jelema G, Noordervliet R, Reymer F, Baelde HA, Paauwe J, et al. Improved performance of the automated slide preparation unit, Sysmex SP-100. Sysmex J Int 2000; 10:71–6.Search in Google Scholar
15 International Council for Standardization in Haematology (ICSH): ICSH reference method for staining of blood and bone marrow by azure B and eosin Y (Romanowsky stain). Br J Haematol 1984; 57:707–10.10.1111/j.1365-2141.1984.tb02949.xSearch in Google Scholar
16 Bennatar L, Flandrin G. Analyse morphométrique, coloration panoptique de May-Grundwald Giemsa et controle de qualité. Annales du Contrôle National de Qualite (AFSSAPS/France) 1999; 16:157–69.Search in Google Scholar
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