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Autoantibodies against oxidized low-density lipoprotein (ox-LDL) and LDL oxidation status

  • Patrizia Brizzi , Giancarlo Tonolo , Graziano Bertrand , Francesca Carusillo , Cristiana Severino , Mario Maioli , Lucia Malaguarnera and Salvatore Musumeci
Published/Copyright: June 1, 2005
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 42 Issue 2

Abstract

Oxidized low-density lipoproteins (ox-LDLs) and their autoantibodies (OLAB) are involved in the development of atherosclerosis in animal models, but their role in humans is still not clear. For this reason we studied 54 patients with β-thalassemia major (TM), as a model of chronically low circulating LDLs with a high level of oxidation; 44 patients with primary hypercholesterolemia, as model of chronically high circulating LDLs; 24 type 2 diabetic mellitus patients (T2DM) before and after 3 months of atorvastatin treatment (20 mg/day), as a model of acute changes in circulating LDLs; and 41 normolipidemic subjects as a control group. ox-LDLs were measured by the determination of baseline diene concentration in the plasma LDL lipidic fraction after 12 hours fasting and were expressed as the amount of conjugated dienes/liter (BDC/l) or BDC/LDL-cholesterol (LDL-C), which indicate respectively LDL oxidation degree and status. OLABwere determined using an enzyme immunoassay and related to LDL oxidation degree (BDC/l). In TM, BDC/l was lower, while BDC/LDL-C was significantly higher, compared to both hypercholesterolemia and normolipidemic subjects. Patients with hypercholesterolemia had higher BDC/l, but lower BDC/LDL-C and OLAB/BDC-l, than normolipidemic subjects. In T2DM patients at diet, BDC/LDL-C and OLAB/BDC-l were lower than in normolipidemic subjects. After 3 months of atorvastatin treatment, BDC/LDL-C and OLAB/BDC-l ratios increased. When all patients were evaluated together, a significant inverse correlation was evident between OLABand either LDL or BDC/l. Our findings suggest that a relationship between OLABtiter and oxidation indices (BDC/l and BDC/LDL-C) does exist and we may speculate that an increase in OLAB/BDC-l ratio might be protective against the risk of atherosclerosis.

Published Online: 2005-6-1
Published in Print: 2004-2-18

Copyright © 2004 by Walter de Gruyter GmbH & Co. KG

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https://doi.org/10.1515/CCLM.2004.030

Articles in the same Issue

  1. Matrix metalloproteinases, inflammation and atherosclerosis: therapeutic perspectives
  2. Regulation of inflammatory responses by activated protein C: the molecular mechanism(s) and therapeutic implications
  3. Increased serum maternal levels of the HER2 oncoprotein p105 ectodomain in preeclampsia
  4. Determination of urinary glyoxal and methylglyoxal by high-performance liquid chromatography
  5. Antioxidants in patients with hyperthyroidism
  6. Biochemical diagnosis of impaired left ventricular ejection fraction - comparison of the diagnostic accuracy of brain natriuretic peptide (BNP) and amino terminal proBNP (NT-proBNP)
  7. Autoantibodies against oxidized low-density lipoprotein (ox-LDL) and LDL oxidation status
  8. Concomitant isolation of protein C inhibitor and unnicked β2-glycoprotein I
  9. Decreased expression of the insulin-like growth factor-I-binding protein-1 (IGFBP-1) phosphoisoform in pre-eclamptic Wharton's jelly and its role in the regulation of collagen biosynthesis
  10. Urine albumin excretion in acute ischaemic stroke is related to serum interleukin-6
  11. Diagnostic use of cerebral and extracerebral oxysterols
  12. Immunoglobulin G Fcγ receptor expression on polymorphonuclear cells in bronchoalveolar lavage fluid of HIV-infected and HIV-seronegative patients with bacterial pneumonia
  13. The discordance rate, a new concept for combining diagnostic decisions with analytical performance characteristics. 2. Defining analytical goals applied to the diagnosis of type 2 diabetes by blood glucose concentrations
  14. Effects of some pre-analytical conditions on the measurement of homocysteine and cysteine in plasma
  15. Testing and validating a homogeneous immunometric assay for interference
  16. Linearity evaluation of analytical methods that count particles
  17. Environmental dichlorodiphenyltrichlorethane or hexachlorobenzene exposure and breast cancer: is there a risk?
  18. Analytical performance and method comparison study of the total homocysteine fluorescence polarization immunoassay (FPIA) on the AxSYM analyzer
  19. Distribution of low-density lipoprotein particle size in healthy Korean adult males
  20. Management of invalid internal controls in the Cobas Amplicor HCV-RNA test using the high-speed centrifugation method
  21. The phenomenon of regression-toward-the-mean
  22. Error messages when calculating chi-square statistics with Microsoft EXCEL®
  23. Meetings and Awards
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