Organic Acids in the Second Morning Urine in a Healthy Swiss Paediatric Population
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Olivier Boulat
Abstract
Organic acid analysis is used for the early detection/exclusion and for the follow-up of inherited disorders of amino acid and organic acid metabolism. Urinary organic acid concentrations in 417 healthy Caucasian children (1 day to 17 years of age) were determined after liquid solid extraction, as their trimethylsilyl derivatives, by gas chromatography and mass spectrometry. Concentrations of most of the organic acids adjusted for creatinine tend to decrease with age. No differences were found between gender except for the Krebs cycle intermediates in the older age groups. In neonates, the immaturity of the neonatal kidney led to a much larger variation of organic acid levels when related to creatinine. The low number of subjects (n = 36−52) per age class resulted in large 95% confidence intervals of the percentiles used for decision. This must be taken into account when using the data for exclusion or diagnosis of disorders.
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Author Index
- Subject Index
- Contents
- Mass Spectrometry in Laboratory Medicine
- Mass Spectrometry-Based Proteomics: Current Status and Potential Use in Clinical Chemistry
- Application of Gas Chromatography-Mass Spectrometry for Analysis of Isoprostanes: Their Role in Cardiovascular Disease
- The Application of Clinical Proteomics to Cancer and other Diseases
- Therapeutic Drug Monitoring of 13 Antidepressant and Five Neuroleptic Drugs in Serum with Liquid Chromatography-Electrospray Ionization Mass Spectrometry
- Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids
- Profiling and in vivo Quantification of Proteins by High Resolution Mass Spectrometry: The Example of Goserelin, an Analogue of Luteinizing Hormone-Releasing Hormone
- Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection
- Analysis of Nitrofuran Metabolites in Food by High-Performance Liquid Chromatography with Tandem Mass Spectrometry Detection
- Automated LC-MS Method for the Fast Stereoselective Determination of Methadone in Plasma
- Proteome Analysis of Diseased Joints from Mice Suffering from Collagen-Induced Arthritis
- Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method
- Organic Acids in the Second Morning Urine in a Healthy Swiss Paediatric Population
- Acknowledgement
- Meetings and Awards
Articles in the same Issue
- Author Index
- Subject Index
- Contents
- Mass Spectrometry in Laboratory Medicine
- Mass Spectrometry-Based Proteomics: Current Status and Potential Use in Clinical Chemistry
- Application of Gas Chromatography-Mass Spectrometry for Analysis of Isoprostanes: Their Role in Cardiovascular Disease
- The Application of Clinical Proteomics to Cancer and other Diseases
- Therapeutic Drug Monitoring of 13 Antidepressant and Five Neuroleptic Drugs in Serum with Liquid Chromatography-Electrospray Ionization Mass Spectrometry
- Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids
- Profiling and in vivo Quantification of Proteins by High Resolution Mass Spectrometry: The Example of Goserelin, an Analogue of Luteinizing Hormone-Releasing Hormone
- Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection
- Analysis of Nitrofuran Metabolites in Food by High-Performance Liquid Chromatography with Tandem Mass Spectrometry Detection
- Automated LC-MS Method for the Fast Stereoselective Determination of Methadone in Plasma
- Proteome Analysis of Diseased Joints from Mice Suffering from Collagen-Induced Arthritis
- Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method
- Organic Acids in the Second Morning Urine in a Healthy Swiss Paediatric Population
- Acknowledgement
- Meetings and Awards
