Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection

Emmanuelle Cognard; Christian Staub

doi:10.1515/CCLM.2003.243

Article

Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection

Emmanuelle Cognard and Christian Staub

Published/Copyright: June 1, 2005

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Clinical Chemistry and Laboratory Medicine (CCLM)

From the journal Volume 41 Issue 12

Abstract

A specific method has been developed for the quantitative determination of nicotine and its major metabolite cotinine in plasma or serum of active and passive smokers. Deuterium-labelled nicotine and cotinine were used as internal standards. The amounts of nicotine and cotinine present in a sample of plasma or serum were extracted with a simple extraction procedure (liquid-liquid or solid-phase extraction). The extracts were analysed by gas chromatography coupled with mass spectrometry using ion-trap detection. The analysis was done in positive chemical ionisation with methanol as the liquid reagent. The method has been demonstrated to be linear up to 1000 μg/l. Limits of quantification for nicotine and cotinine are 10 and 5 μg/l, respectively with liquid-liquid extraction, and 1 μg/l for each of the compounds with solid-phase extraction. The present method has been applied to several real cases.

Published Online: 2005-06-01

Published in Print: 2003-12-04

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https://doi.org/10.1515/CCLM.2003.243