Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method
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Debora Tagliacozzi
Abstract
Bile acids play a pivotal role in the metabolism of cholesterol and lipids. Their blood concentrations are important prognostic and diagnostic indicators of hepatobiliary and intestinal dysfunction. This class of molecules comprises a heterogeneous group of compounds with a common cholesterol scaffold. Recently, the introduction of liquid chromatography coupled to tandem mass spectrometry methods has revealed an innovative path in the quantisation of specific bile acids in biological specimens. A robust and sensitive method has been developed based on high performance liquid chromatography separation coupled to an electrospray triple-quadrupole mass spectrometer. Human plasma samples were analysed on a C18 reverse-phase column. The elution profiles were monitored in multiple reaction-monitoring mode, quantifying and identifying each analyte by its own unique precursor to product patterns. A linear correlation over a broad range of bile acid concentrations (0.1–100 μM) was observed. The average recovery period for all of the analysed bile acids was 98±3%. Intra-day and inter-day precision averages were 2% and 5.4%, respectively. The determination was achieved within a single chromatographic run for all unconjugated, glycine-and taurine-conjugated isomeric forms of bile acids. As a proof of principle this method has been validated on a small subset of cholestatic patients (n = 7) and compared to appropriate clinical controls (n = 10).
Based upon our encouraging experimental results, the described HPLC separation coupled to tandem mass spectrometry method for the analysis of bile acids in biological samples is deemed a robust and accurate procedure. Consequently, we propose this technique as a suitable candidate method for the identification and quantitation of bile acids in routine analysis.
Copyright (c) 2003 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Author Index
- Subject Index
- Contents
- Mass Spectrometry in Laboratory Medicine
- Mass Spectrometry-Based Proteomics: Current Status and Potential Use in Clinical Chemistry
- Application of Gas Chromatography-Mass Spectrometry for Analysis of Isoprostanes: Their Role in Cardiovascular Disease
- The Application of Clinical Proteomics to Cancer and other Diseases
- Therapeutic Drug Monitoring of 13 Antidepressant and Five Neuroleptic Drugs in Serum with Liquid Chromatography-Electrospray Ionization Mass Spectrometry
- Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids
- Profiling and in vivo Quantification of Proteins by High Resolution Mass Spectrometry: The Example of Goserelin, an Analogue of Luteinizing Hormone-Releasing Hormone
- Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection
- Analysis of Nitrofuran Metabolites in Food by High-Performance Liquid Chromatography with Tandem Mass Spectrometry Detection
- Automated LC-MS Method for the Fast Stereoselective Determination of Methadone in Plasma
- Proteome Analysis of Diseased Joints from Mice Suffering from Collagen-Induced Arthritis
- Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method
- Organic Acids in the Second Morning Urine in a Healthy Swiss Paediatric Population
- Acknowledgement
- Meetings and Awards
Articles in the same Issue
- Author Index
- Subject Index
- Contents
- Mass Spectrometry in Laboratory Medicine
- Mass Spectrometry-Based Proteomics: Current Status and Potential Use in Clinical Chemistry
- Application of Gas Chromatography-Mass Spectrometry for Analysis of Isoprostanes: Their Role in Cardiovascular Disease
- The Application of Clinical Proteomics to Cancer and other Diseases
- Therapeutic Drug Monitoring of 13 Antidepressant and Five Neuroleptic Drugs in Serum with Liquid Chromatography-Electrospray Ionization Mass Spectrometry
- Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids
- Profiling and in vivo Quantification of Proteins by High Resolution Mass Spectrometry: The Example of Goserelin, an Analogue of Luteinizing Hormone-Releasing Hormone
- Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection
- Analysis of Nitrofuran Metabolites in Food by High-Performance Liquid Chromatography with Tandem Mass Spectrometry Detection
- Automated LC-MS Method for the Fast Stereoselective Determination of Methadone in Plasma
- Proteome Analysis of Diseased Joints from Mice Suffering from Collagen-Induced Arthritis
- Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method
- Organic Acids in the Second Morning Urine in a Healthy Swiss Paediatric Population
- Acknowledgement
- Meetings and Awards
