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Automated LC-MS Method for the Fast Stereoselective Determination of Methadone in Plasma

  • Sandrine Souverain , Chin Eap , Jean-Luc Veuthey and Serge Rudaz
Published/Copyright: June 1, 2005
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 41 Issue 12

Abstract

Methadone (MTD) is a chiral drug widely used for the treatment of opioid dependence for which a rapid analytical method for the determination of each enantiomer would be advantageous. In order to improve method sensitivity and to automate the entire analytical process, a column-switching configuration has been developed. An online extraction system coupled to a cellulose-based chiral stationary phase (CSP), namely Chiralcel OJ-R, was used and detection was performed by mass spectrometry. Fifty μl of plasma were injected into the liquid chromatography-mass spectrometry (LC-MS) system after addition of acetonitrile (ACN) containing methadone deuterated D9 (MTD-D9) (internal standard) and centrifugation. For the rapid extraction step, a large particle size support was selected. A baseline separation of MTD enantiomers was obtained in less than 12 min. Trueness and precision were evaluated with control samples at 500 ng/ml of (R,S)-methadone. Trueness values were 106.6% and 103.0% for (R)-MTD and (S)-MTD, respectively, with a coefficient of variation inferior to 2.5% for both compounds. Finally, a good concordance was found using this method for analysis of plasma samples from patients in maintenance treatment as compared to a previously described HPLC-UV method after liquid-liquid extraction.

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Published Online: 2005-06-01
Published in Print: 2003-12-04

Copyright © 2003 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Author Index
  2. Subject Index
  3. Contents
  4. Mass Spectrometry in Laboratory Medicine
  5. Mass Spectrometry-Based Proteomics: Current Status and Potential Use in Clinical Chemistry
  6. Application of Gas Chromatography-Mass Spectrometry for Analysis of Isoprostanes: Their Role in Cardiovascular Disease
  7. The Application of Clinical Proteomics to Cancer and other Diseases
  8. Therapeutic Drug Monitoring of 13 Antidepressant and Five Neuroleptic Drugs in Serum with Liquid Chromatography-Electrospray Ionization Mass Spectrometry
  9. Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids
  10. Profiling and in vivo Quantification of Proteins by High Resolution Mass Spectrometry: The Example of Goserelin, an Analogue of Luteinizing Hormone-Releasing Hormone
  11. Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection
  12. Analysis of Nitrofuran Metabolites in Food by High-Performance Liquid Chromatography with Tandem Mass Spectrometry Detection
  13. Automated LC-MS Method for the Fast Stereoselective Determination of Methadone in Plasma
  14. Proteome Analysis of Diseased Joints from Mice Suffering from Collagen-Induced Arthritis
  15. Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method
  16. Organic Acids in the Second Morning Urine in a Healthy Swiss Paediatric Population
  17. Acknowledgement
  18. Meetings and Awards
https://doi.org/10.1515/CCLM.2003.245

Articles in the same Issue

  1. Author Index
  2. Subject Index
  3. Contents
  4. Mass Spectrometry in Laboratory Medicine
  5. Mass Spectrometry-Based Proteomics: Current Status and Potential Use in Clinical Chemistry
  6. Application of Gas Chromatography-Mass Spectrometry for Analysis of Isoprostanes: Their Role in Cardiovascular Disease
  7. The Application of Clinical Proteomics to Cancer and other Diseases
  8. Therapeutic Drug Monitoring of 13 Antidepressant and Five Neuroleptic Drugs in Serum with Liquid Chromatography-Electrospray Ionization Mass Spectrometry
  9. Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids
  10. Profiling and in vivo Quantification of Proteins by High Resolution Mass Spectrometry: The Example of Goserelin, an Analogue of Luteinizing Hormone-Releasing Hormone
  11. Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection
  12. Analysis of Nitrofuran Metabolites in Food by High-Performance Liquid Chromatography with Tandem Mass Spectrometry Detection
  13. Automated LC-MS Method for the Fast Stereoselective Determination of Methadone in Plasma
  14. Proteome Analysis of Diseased Joints from Mice Suffering from Collagen-Induced Arthritis
  15. Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method
  16. Organic Acids in the Second Morning Urine in a Healthy Swiss Paediatric Population
  17. Acknowledgement
  18. Meetings and Awards
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