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Molecular Cloning, Sequencing and Functional Expression of Porcine Thyrotropin (TSH) Receptor cDNA
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Makoto Igarashi
Published/Copyright:
June 1, 2005
Abstract
We directly sequenced PCR fragments amplified from a porcine thyroid cDNA library with various synthetic primers based on the nucleotide sequence of bovinethyrotropin receptor (bTSHR) cDNA. A nucleotide sequence of 2410 bp was determined and its deduced amino acid sequence contained an open reading frame coding 764 amino acids, which showed 91.8% and 89.5% similarity to bTSHR and human TSH receptor, respectively. A hydrophobicity plot of the porcine protein suggested that it had a putative signal peptide and seven transmembrane domains. Chinese hamster ovary cells stably transfected with the cDNA could be specifically bound to bovine TSH (bTSH) and increased the intracellular cAMP level in response to bTSH and Graves' disease patients' sera stimulation.
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Published Online: 2005-06-01
Published in Print: 2003-06-17
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
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- Meetings and Awards
Articles in the same Issue
- Editorial
- Biomedical Applications of Capillary Electrophoresis
- Capillary Electrophoresis – A High Performance Analytical Separation Technique
- The Analysis of Carbohydrate-Deficient Transferrin, Marker of Chronic Alcoholism, Using Capillary Electrophoresis
- Capillary Electrophoresis of Hemoglobin
- Capillary Electrophoresis for the Determination of Organic Acidurias in Body Fluids: A Review
- Separation of Serum Proteins by Automated Capillary Zone Electrophoresis
- Is Capillary Electrophoresis a Method of Choice for Systematic Toxicological Analysis?
- Capillary Zone Electrophoresis of Serum Proteins: Effects of Changed Analytical Conditions
- Type-Specific Detection of Human Papillomaviruses in a Routine Laboratory Setting – Improved Sensitivity and Specificity of PCR and Sequence Analysis Compared to Direct Hybridisation
- Identification of Lipoproteins of Intestinal Origin in Human Atherosclerotic Plaque
- Molecular Cloning, Sequencing and Functional Expression of Porcine Thyrotropin (TSH) Receptor cDNA
- Are Results of Fibrinogen Measurements Transferable?
- Comparison of Several Point-of-Care Testing (POCT) Glucometers with an Established Laboratory Procedure for the Diagnosis of Type 2 Diabetes Using the Discordance Rate. A New Statistical Approach
- Association between Increased Serum Cholesterol and Signs of Depressive Mood
- Report from the Second European Symposium on Clinical Laboratory and in vitro Diagnostic Industry. Physiological Reference Values: A Shared Business? Barcelona, 67 February 2003
- Meetings and Awards
