Capillary Electrophoresis of Hemoglobin
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Margaret Jenkins
Abstract
Capillary electrophoresis (CE) has been used in a variety of in-house capillary isoelectric focusing (CIEF) and capillary zone electrophoresis (CZE) assays for the detection of hemoglobin (Hb) variants and the quantitation of HbA2 and HbF. A commercial kit has also been produced for the analysis of hemoglobin variants and thalassemia screening. Though CE methods have been shown to be able to detect many variants, final identification of the variant needs specialized testing such as DNA technology. Over the past 2 years, many instruments that had been used for these hemoglobin variant screening and thalassemia assays have been withdrawn from sale. Although CE HbA1c analysis is available, it cannot compete in turnaround time or cost with automated HPLC commercial instruments that give accurate HbA1c results in 3 or 4 minutes. Hence we do not anticipate a bright future for the analysis of hemoglobin by CE.
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Editorial
- Biomedical Applications of Capillary Electrophoresis
- Capillary Electrophoresis – A High Performance Analytical Separation Technique
- The Analysis of Carbohydrate-Deficient Transferrin, Marker of Chronic Alcoholism, Using Capillary Electrophoresis
- Capillary Electrophoresis of Hemoglobin
- Capillary Electrophoresis for the Determination of Organic Acidurias in Body Fluids: A Review
- Separation of Serum Proteins by Automated Capillary Zone Electrophoresis
- Is Capillary Electrophoresis a Method of Choice for Systematic Toxicological Analysis?
- Capillary Zone Electrophoresis of Serum Proteins: Effects of Changed Analytical Conditions
- Type-Specific Detection of Human Papillomaviruses in a Routine Laboratory Setting – Improved Sensitivity and Specificity of PCR and Sequence Analysis Compared to Direct Hybridisation
- Identification of Lipoproteins of Intestinal Origin in Human Atherosclerotic Plaque
- Molecular Cloning, Sequencing and Functional Expression of Porcine Thyrotropin (TSH) Receptor cDNA
- Are Results of Fibrinogen Measurements Transferable?
- Comparison of Several Point-of-Care Testing (POCT) Glucometers with an Established Laboratory Procedure for the Diagnosis of Type 2 Diabetes Using the Discordance Rate. A New Statistical Approach
- Association between Increased Serum Cholesterol and Signs of Depressive Mood
- Report from the Second European Symposium on Clinical Laboratory and in vitro Diagnostic Industry. Physiological Reference Values: A Shared Business? Barcelona, 67 February 2003
- Meetings and Awards
Articles in the same Issue
- Editorial
- Biomedical Applications of Capillary Electrophoresis
- Capillary Electrophoresis – A High Performance Analytical Separation Technique
- The Analysis of Carbohydrate-Deficient Transferrin, Marker of Chronic Alcoholism, Using Capillary Electrophoresis
- Capillary Electrophoresis of Hemoglobin
- Capillary Electrophoresis for the Determination of Organic Acidurias in Body Fluids: A Review
- Separation of Serum Proteins by Automated Capillary Zone Electrophoresis
- Is Capillary Electrophoresis a Method of Choice for Systematic Toxicological Analysis?
- Capillary Zone Electrophoresis of Serum Proteins: Effects of Changed Analytical Conditions
- Type-Specific Detection of Human Papillomaviruses in a Routine Laboratory Setting – Improved Sensitivity and Specificity of PCR and Sequence Analysis Compared to Direct Hybridisation
- Identification of Lipoproteins of Intestinal Origin in Human Atherosclerotic Plaque
- Molecular Cloning, Sequencing and Functional Expression of Porcine Thyrotropin (TSH) Receptor cDNA
- Are Results of Fibrinogen Measurements Transferable?
- Comparison of Several Point-of-Care Testing (POCT) Glucometers with an Established Laboratory Procedure for the Diagnosis of Type 2 Diabetes Using the Discordance Rate. A New Statistical Approach
- Association between Increased Serum Cholesterol and Signs of Depressive Mood
- Report from the Second European Symposium on Clinical Laboratory and in vitro Diagnostic Industry. Physiological Reference Values: A Shared Business? Barcelona, 67 February 2003
- Meetings and Awards
