The Analysis of Carbohydrate-Deficient Transferrin, Marker of Chronic Alcoholism, Using Capillary Electrophoresis
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Birgitte Wuyts
Abstract
Carbohydrate-deficient transferrin (CDT) is currently considered to be the best available marker for the diagnosis of chronic alcoholism. A large variety of methods have been developed, demonstrating the need for standardisation. Commercially available anion-exchange chromatographic-based assays are easy to use and require no specialised, expensive instruments. However, these methods cannot identify genetic transferrin variants or the carbohydrate-deficient glycoprotein syndrome. In 1989, a capillary isoelectric focusing method was developed for quantitative measurement of CDT. Despite the optimal resolution, this method is not easily applied in a clinical routine environment due to the complexity of analysis. Capillary electrophoresis in a polymer network using coated capillaries allowed full resolution of the sialoforms of human transferrin. The drawbacks due to an expensive and time-consuming sample preparation were eliminated when a method in neat serum was developed. Capillary zone electrophoresis allowed full resolution of the transferrin isoforms with a high analytical performance in a short analysis time thanks to a strong electroosmotic flow. Genetic transferrin variants were easily detected, avoiding false-positive results. Also, using capillary zone electrophoresis, it was shown that CDT is a suitable marker of chronic alcohol abuse detection in transferrin CD (common/cathodal) variants.
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Editorial
- Biomedical Applications of Capillary Electrophoresis
- Capillary Electrophoresis – A High Performance Analytical Separation Technique
- The Analysis of Carbohydrate-Deficient Transferrin, Marker of Chronic Alcoholism, Using Capillary Electrophoresis
- Capillary Electrophoresis of Hemoglobin
- Capillary Electrophoresis for the Determination of Organic Acidurias in Body Fluids: A Review
- Separation of Serum Proteins by Automated Capillary Zone Electrophoresis
- Is Capillary Electrophoresis a Method of Choice for Systematic Toxicological Analysis?
- Capillary Zone Electrophoresis of Serum Proteins: Effects of Changed Analytical Conditions
- Type-Specific Detection of Human Papillomaviruses in a Routine Laboratory Setting – Improved Sensitivity and Specificity of PCR and Sequence Analysis Compared to Direct Hybridisation
- Identification of Lipoproteins of Intestinal Origin in Human Atherosclerotic Plaque
- Molecular Cloning, Sequencing and Functional Expression of Porcine Thyrotropin (TSH) Receptor cDNA
- Are Results of Fibrinogen Measurements Transferable?
- Comparison of Several Point-of-Care Testing (POCT) Glucometers with an Established Laboratory Procedure for the Diagnosis of Type 2 Diabetes Using the Discordance Rate. A New Statistical Approach
- Association between Increased Serum Cholesterol and Signs of Depressive Mood
- Report from the Second European Symposium on Clinical Laboratory and in vitro Diagnostic Industry. Physiological Reference Values: A Shared Business? Barcelona, 67 February 2003
- Meetings and Awards
Articles in the same Issue
- Editorial
- Biomedical Applications of Capillary Electrophoresis
- Capillary Electrophoresis – A High Performance Analytical Separation Technique
- The Analysis of Carbohydrate-Deficient Transferrin, Marker of Chronic Alcoholism, Using Capillary Electrophoresis
- Capillary Electrophoresis of Hemoglobin
- Capillary Electrophoresis for the Determination of Organic Acidurias in Body Fluids: A Review
- Separation of Serum Proteins by Automated Capillary Zone Electrophoresis
- Is Capillary Electrophoresis a Method of Choice for Systematic Toxicological Analysis?
- Capillary Zone Electrophoresis of Serum Proteins: Effects of Changed Analytical Conditions
- Type-Specific Detection of Human Papillomaviruses in a Routine Laboratory Setting – Improved Sensitivity and Specificity of PCR and Sequence Analysis Compared to Direct Hybridisation
- Identification of Lipoproteins of Intestinal Origin in Human Atherosclerotic Plaque
- Molecular Cloning, Sequencing and Functional Expression of Porcine Thyrotropin (TSH) Receptor cDNA
- Are Results of Fibrinogen Measurements Transferable?
- Comparison of Several Point-of-Care Testing (POCT) Glucometers with an Established Laboratory Procedure for the Diagnosis of Type 2 Diabetes Using the Discordance Rate. A New Statistical Approach
- Association between Increased Serum Cholesterol and Signs of Depressive Mood
- Report from the Second European Symposium on Clinical Laboratory and in vitro Diagnostic Industry. Physiological Reference Values: A Shared Business? Barcelona, 67 February 2003
- Meetings and Awards
