Intermethod Variation in Serum Carcinoembryonic Antigen (CEA) Measurement. Fresh Serum Pools and Control Materials Compared
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Roberto Dominici
Abstract
This study was undertaken to evaluate the feasibility of using commercial control materials in a regional external quality assessment scheme (EQAS) for serum carcinoembryonic antigen (CEA) measurement. We have assessed the commutability of 12 commercial control materials using five automated immunochemical systems. We compared the intermethod behavior of the materials with that of 12–14 patient serum pools. In a total of 48 comparisons (12 materials × 4 pairs of analytical systems), seven instances of noncommutability were apparent, as shown by normalized residuals falling outside the ±3 interval. The use of non-commutable materials generates two negative effects. In EQAS, the interlaboratory variation recorded is not representative of the variation expected in the assay of patient sera; in interlaboratory harmonization programs, recalibration with non-commutable materials increases, instead of decreasing, the interlaboratory variation. Both these effects were shown to occur in CEA measurement with the tested materials and systems. The materials planned to be used in our EQAS turned out to be commutable: this gave us the guarantee of measuring realistic interlaboratory variation values, although the check for commutability should be extended to all the analytical systems used by the participants in the scheme.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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- Recommendations for the Routine Use of Pancreatic Amylase Measurement instead of Total Amylase for the Diagnosis and Monitoring of Pancreatic Pathology
- Bovine Spongiform Encephalopathy: Are the Cows Mad or Full of Carbohydrates?
- Plasma Total Antioxidant Capacity in Hemodialyzed Patients and Its Relationships to other Biomarkers of Oxidative Stress and Lipid Peroxidation
- Effects of Propofol and Taurine on Intracellular Free Amino Acid Profiles and Immune Function Markers in Neutrophils In Vitro
- Genotyping of Hemochromatosis-Associated Mutations in the HFE Gene by PCR-RFLP and a Novel Revers Hybridization Method
- Comparison of Different CD71 Monoclonal Antibodies for Enrichment of Fetal Cells from Maternal Blood
- The Effects of Marathon Swimming on Serum Leptin and Plasma Neuropeptide Y Levels
- Renal Function Exerts only a Minor Influence on High Plasma Homocysteine Concentrations in Patients with Acute Coronary Syndromes
- Changes of Biochemical Bone Markers during the Menopausal Transition
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- Experience with External Quality Assessment of Drugs of Abuse Testing in the Lombardy Region in Italy
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- Intermethod Variation in Serum Carcinoembryonic Antigen (CEA) Measurement. Fresh Serum Pools and Control Materials Compared
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- A Two-Center Evaluation of the Blood Gas Immediate Response Mobile Analyzer (IRMA)
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