The Effects of Affinity-Purified Anti-DNA Antibodies Derived from Patients with Systemic Lupus Erythematosus on the Fluorescent Antinuclear Antibody Assay Using HEp-2 Cells
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Kimihiro Suzuki
Abstract
The aim of this study was to clarify the effects of anti-dsDNA antibodies on the titer and the nuclear staining pattern(s) in a fluorescent antinuclear antibody (FANA) assay using HEp-2 cells. Anti-dsDNA derived from 14 patients with systemic lupus erythematosus (SLE) was individually affinity-purified. The anti-dsDNA titer of the purified anti-dsDNA solution was measured by radioimmunoassay (RIA) or by enzyme-linked immunosorbent assay (ELISA). In the FANA assay, the anti-dsDNA solution was diluted in a stepwise manner and its titer was expressed by the endpoint dilution. The nuclear staining pattern in the anti-dsDNA solution was examined at the 1:5 and 1:20 dilutions and at the endpoint dilution. The anti-dsDNA titers of the affinity-purified anti-dsDNA solution were high enough (13 to 126 IU/ml) to be measured by RIA. However, the antinuclear antibody (ANA) titers of this solution were relatively low: 1:20 to 1:320. In the study of nuclear staining the peripheral pattern was observed in nine of the 14 cases at a 1:5 dilution. However, at the endpoint dilution, all cases exhibited the homogeneous pattern. These findings indicate that in the FANA assay using HEp-2 cells, 1) although serum samples show high anti-dsDNA titers by RIA or by ELISA, the antibodies' direct contribution to ANA titers is limited, and 2) when samples reveal a homogeneous staining pattern at the endpoint dilution, this suggests the presence of anti-dsDNA.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Editorial
- Growing Significance of Myeloperoxidase in Non-infectious Diseases
- Serum Paraoxonase Activity and the Extent of Lipid Peroxidation Are not Affected by Increased Levels of Human Apolipoprotein A-I: Studies in Transgenic Mice
- Relationship between the Sialic Acid Content of Low-Density Lipoprotein (LDL) and Autoantibodies to Oxidized LDL in the Plasma of Healthy Subjects and Patients with Atherosclerosis
- The Presence of Heparin-Platelet Factor 4 Antibodies as a Marker of Hypercoagulability during Hemodialysis
- Clinical Evaluation of Changes in the Morphology of Eosinophils
- The n-3 and n-6 Polyunsaturated Fatty Acid Composition of Plasma Phospholipids in Pregnant Women and Their Infants. Relationship with Maternal Linoleic Acid Intake
- Quantification of Sirolimus by Liquid Chromatography-Tandem Mass Spectrometry Using On-Line Solid-Phase Extraction
- The Effects of Affinity-Purified Anti-DNA Antibodies Derived from Patients with Systemic Lupus Erythematosus on the Fluorescent Antinuclear Antibody Assay Using HEp-2 Cells
- Relationships between Serum Markers of Monocyte/Macrophage Activation in Type 1 Gaucher's Disease
- Gender Differences in C-Reactive Protein Concentrations - Confirmation with Two Sensitive Methods
- Serum Cholinesterase Activity in Patients with Burns
- Serum Lipids and Apolipoproteins in Patients with Psoriasis
- Reference Intervals for a Complete Blood Count Determined on different Automated Haematology Analysers: Abx Pentra 120 Retic, Coulter Gen-S, Sysmex SE 9500, Abbott Cell Dyn 4000 and Bayer Advia 120
- Science, Alchemy and Light: Paintings by Joseph Wright of Derby
- Approved IFCC Reference Method for the Measurement of HbA1c in Human Blood
- Meetings and Awards
