A Simple HPLC Method for Monitoring Mycophenolic Acid and Its Glucuronidated Metabolite in Transplant Recipients
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Gernot Seebacher
Abstract
Mycophenolic acid (MPA) is nowadays in broad clinical use as a substitute for azathioprine. An immunoassay for MPA recently received approval for clinical applications. The high performance liquid chromatography (HPLC) assay for measuring MPA and its glucuronide conjugate (MPAG) we describe here is not only rapid and simple but also extremely sensitive at plasma levels obtained during standard immunosuppressive regimens. The determination of MPAG is possible without any change of the chromatographic conditions (detection wavelength of 214 nm, mobile phase: acetonitrile and 50 mmol/l o-phosphoric acid (50:50, V/V), run time: 15 min). The required equipment is a standard HPLC system including a simple UV-detector. Sample volume of 400 μl is required for both determinations. Detection limit is 0.25 μmol/l for MPA and 5 μmol/l for MPAG. Linearity is excellent for serial dilutions (0.5–25 μmol/l for MPA, 25–500 μmol/l for MPAG) and high accuracies favour the method described.
More than 2000 plasma samples tested for MPA in patients after heart transplantation within one year and more than 500 samples for MPAG underline the clinical applicability of this assay.
Copyright (c)1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Serum Amyloid A (SAA): a Concise Review of Biology, Assay Methods and Clinical Usefulness
- Serum Cystatin C as an Endogenous Marker of the Renal Function – a Review
- Improved Fluorescent PCR-Based Assay for Sizing CGG Repeats at the FRAXA Locus
- The Influence of Radiation and Chemotherapy-Related DNA Strand Breaks on Carcinogenesis: An Evaluation
- A Simple HPLC Method for Monitoring Mycophenolic Acid and Its Glucuronidated Metabolite in Transplant Recipients
- The Activities of Some Glycosaminoglycan-Degrading Enzymes in the Wall of the Umbilical Cord Artery and Their Alteration in Edema, Proteinuria, Hypertension (EPH)-Gestosis
- Endothelin-1- and Endothelin-Receptors in Lung Biopsies of Patients with Pulmonary Hypertension due to Congenital Heart Disease
- Increased Serum Neopterin Concentrations in Patients with Alzheimer’s Disease
- Biomarkers of Bone Turnover and Bone Mineral Density in Hyperprolactinemic Amenorrheic Women
- Age Relationships and Sex Differences in Serum Levels of Pregnenolone and 17-Hydroxypregnenolone in Normal Subjects
- A Study of Hypermagnesaemia in a Hospital Population
- Multiple Regression Analysis of Interference Effects from a Hemoglobin-Based Oxygen Carrier Solution
- Serum Ionized Magnesium: Comparison of Results Obtained with Three Ion-Selective Analyzers
- Automated Prozone Effect Detection in Ferritin Homogeneous Immunoassays Using Neural Network Classifiers
- Recent Activities of EC4 in the Harmonization of Clinical Chemistry in the European Union
- Preventing Coronary Artery Disease. Cardioprotective Therapeutics in Practice. Edited by M. J. Kendall and R. C. Horton
- Will Medical Schools Remain what They Are? Medical Education in the Millennium. Edited by Brian Jolly and Lesley Rees
