Photodynamic Effects In Vitro in Fresh GynecologicTumors Analyzed with a Bioluminescence Method
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Viola Schlosser
Abstract
Photodynamic therapy (PDT) is a promising alternative method for clinical cancer treatment. In the present study, cells from four breast carcinomas, seven ovarian carcinomas of various stages of differentiation, and ascites from a diffuse metastatic tumor were treated by PDT in vitro. Tetra(m-hydroxyphenyl)-chlorin (m-THPC) was used as the photosensitizer. Surviving cell rate was evaluated by the ATP-Cell-Viability-Assay (ATP-CVA), which measures light production as an interaction of intracellular ATP with the luciferin-luciferase complex. The most effective PDT of the tumor cells was achieved at an m-THPC concentration of 0.2 μg/ml following incubation of the cells with photosensitizer for 24 hours. PDT toxicity resulted in a cell survival rate of 1 % to 42 % compared to untreated control cells (survival rate of control = 100 %). The inhibitor concentration IC50 of m-THPC was determined both in the dark (dark toxicity) and in combination with laser irradiation. IC50 was defined as the concentration of photosensitizer which caused 50 % of cell death. The IC50 values were heterogeneous in all tumor specimens examined. IC50 values for dark toxicity were on average 0.14 μg m-THPC/ml for primary ovarian carcinoma, 2.16 μg m-THPC ml for refractory ovarian carcinoma and 0.3 μg m-THPC/ml for breast carcinoma. After PDT, average IC50 value for refractory ovarian carcinoma was 0.04 μg m-THPC/ml, for primary ovarian carcinoma 0.05 μg m-THPC/ml and for breast carcinoma 0.03 μg m-THPC/ml. These data might indicate that clinical PDT of gynecological carcinoma requires individual treatment conditions to achieve optimal results.
Copyright (c)1999 by Walter de Gruyter GmbH & Co. KG
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