The Automation of Sediment Urinalysis Using a New Urine Flow Cytometer (UF-100™)
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Domenico Fenili
Abstract
The UF-100™ analyser is a fully automated instrument that stains the DNA and the membranes of the formed elements in native urine. The sample then passes as a laminar flow through a laser beam and light scattering, fluorescence and impedance are measured. The main purpose of the present work was to assess the analytical performance and the accuracy of the measurements of the UF-100™ analyser.
No carryover was observed, while the linearity was higher then the upper limit (40000 total particles μl−1) suggested by the manufacturer. The within-run imprecision was low, ranging from 17.7 % to 2.4 % and was up to threefold better than manual microscopy.
Comparison of results obtained by sediment microscopy (performed according to National Committee for Clinical Laboratory Standards (NCCLS) recommendations) and by the UF-100™ analyser showed a linear correlation with r = 0.833 for erythrocytes, r = 0.934 for leukocytes, r = 0.880 for epithelial cells and r = 0.40 for casts.
To evaluate the reliability of the UF-100™ analyser in detecting bacteria we compared the results with the microbial culture (n = 608). Using a cut-off value of bacterial count above 1800 μl−1 and at leukocyte count above 45 μl−1, the analyser detected positive cultures with a sensitivity of 87 % and a specificity of 80 %.
In conclusion, the UF-100™ analyser can improve the work flow, increasing the output of urinalysis by reducing the number of specimens submitted for microscopy. Also the method provides reliable information for the identification of urinary tract inflammation and bacterial infection.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Author Index
- Contents
- Subject Index
- Polymorphisms of Coagulation Factor Genes a Review
- Urinalysis-Challenges by New Medical Needs and Advanced Technologies
- The Automation of Sediment Urinalysis Using a New Urine Flow Cytometer (UF-100™)
- Urinary Microscopy as Seen by Nephrologists
- Measurement of Urine Relative Density Using Refractometer and Reagent Strips
- Dry Chemistry Urinalysis of Pathological Proteinuria
- Physiopathology of Proteinuria and Laboratory Diagnostic Strategy Based on Single Protein Analysis
- Microalbuminuria in Diabetes
- European Multicentre Evaluation of the Super Aution SA-4220 Urinalysis Analyser
- Optimized Detection of DNA Point Mutations by Double Gradient Denaturing Gradient Gel
- Is the Association of Serum Lipase with β2-Microglobulin or C-Reactive Protein Useful for Establishing the Diagnosis and Prognosis of Patients with Acute Pancreatitis?
- Analytical and Clinical Performance of an Automated Immunoassay System (Immulite®) for Estradiol in Serum
- Evaluation of the Activated Partial Thrombo-plastin Time (APTT) Sensitivity to Heparin Using Five Commercial Reagents: Implications for Therapeutic Monitoring
- An Alternative Analysis for Crossover Studies that Accounts for Between-Group Disparities in Drug Response
Articles in the same Issue
- Author Index
- Contents
- Subject Index
- Polymorphisms of Coagulation Factor Genes a Review
- Urinalysis-Challenges by New Medical Needs and Advanced Technologies
- The Automation of Sediment Urinalysis Using a New Urine Flow Cytometer (UF-100™)
- Urinary Microscopy as Seen by Nephrologists
- Measurement of Urine Relative Density Using Refractometer and Reagent Strips
- Dry Chemistry Urinalysis of Pathological Proteinuria
- Physiopathology of Proteinuria and Laboratory Diagnostic Strategy Based on Single Protein Analysis
- Microalbuminuria in Diabetes
- European Multicentre Evaluation of the Super Aution SA-4220 Urinalysis Analyser
- Optimized Detection of DNA Point Mutations by Double Gradient Denaturing Gradient Gel
- Is the Association of Serum Lipase with β2-Microglobulin or C-Reactive Protein Useful for Establishing the Diagnosis and Prognosis of Patients with Acute Pancreatitis?
- Analytical and Clinical Performance of an Automated Immunoassay System (Immulite®) for Estradiol in Serum
- Evaluation of the Activated Partial Thrombo-plastin Time (APTT) Sensitivity to Heparin Using Five Commercial Reagents: Implications for Therapeutic Monitoring
- An Alternative Analysis for Crossover Studies that Accounts for Between-Group Disparities in Drug Response
