Validation of an Automated Enzyme Immunoassay for Interleukin-6 for Routine Clinical Use
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Abstract
Serum levels of Interleukin-6 (IL-6), a proinflammatory cytokine, are increased in early stages of inflammatory diseases such as infection and sepsis. Assay systems which permit its measurement within a few hours and as a single measurement have not been reported so far. We therefore evaluated a now commercially available automated method for IL-6 measurement on the Cobas Core® immunological analyzer (Roche Diagnostic Systems) which enables single IL-6 measurement within about 1 hour. The automated assay correlates well with an established, manual microtiter plate assay (Biosource GmbH) which uses the same antibodies and reagents (r=0.98). Accuracy of the automated method was established by adding known amounts of IL-6 international reference preparation. Recovery of the international standard was in the range of 92–104 %. The automated assay had a precision of singletons below 6% and was linear up to 2800 pg/ml. This automated assay provides a suitable, convenient and time saving method for measurement of IL-6 serum levels in the routine clinical laboratory.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Kinetic Study of Apo B100 Containing Lipoprotein Metabolism Using Amino Acids Labeled with Stable Isotopes: Methodological Aspects
- Activated Immune System in Patients with Huntington's Disease
- Elevated Group 11 Phospholipase A2 Mass Concentration in Serum and Colonic Mucosa in Crohn's Disease
- Determination of pH in Human Erythrocytes. Sources of Systematic Error
- Rapid Molecular Diagnosis of Erythropoietic Protoporphyria among Swiss Patients
- Biochemical Evaluation of Oxidative Stress in Propylthiouracil Treated Hyperthyroid Patients. Effects of Vitamin C Supplementation
- Effect of Aminoguanidine on Erythrocyte Lipid Peroxiclation and Activities of Antioxidant Enzymes in Experimental Diabetes
- Normalizing Intermethod Free Triiodothyronine Patient Results: Need for Commutable Materials
- Evaluation of a Predictive Kinetic Procedure for the Enzymatic Measurement of Creatinine in Serum
- Pre-Evaluation and System Optimization of the Elecsys® Thyroid Electrochemiluminescence Immunoassays
- Validation of an Automated Enzyme Immunoassay for Interleukin-6 for Routine Clinical Use
- First Experience with the REMEDi HS Urine Benzodiazepine Assay
- Rapid Variations of the Free Prostate-Specific Antigen Fraction in Serum
- Are the Preanalytical Factors Underestimated in Clinical Studies?
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